Detection of bacterial cells by impedance spectra via fluidic electrodes in a microfluidic device

Zhu, Tao; Pei, Zhenhua; Huang, Jianyong; Xiong, Chunyang; Shi, Shenggen; Fang, Jing

doi:10.1039/b925968f

Cited by 29 publications

(11 citation statements)

References 31 publications

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“…The detection limit was also shown comparable with many label-free immunosensors for detection of pathogenic bacteria [5]. Zhu and co-workers [18] recently reported a linear relationship between the impedance and the logarithmic value of the bacterial concentration in certain cell concentration ranges for Porphyromonas gingivalis and Escherichia coli. Different from these studies, another study has demonstrated that use of bacterial impedance together with a dielectrophoretic manipulation can differentiate between different Bacillus species through monitoring current change caused by different species [19].…”

supporting

confidence: 64%

Development of a novel conductance-based technology for environmental bacterial sensing

Shen

Tan

et al. 2013

Chin. Sci. Bull.

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In this study, a simple impedance based technology for measuring bacterial concentrations was developed. The measurement system includes the signal amplification, copper probes and a sample loader. During the experiments, the conductance of Bacillus subtilis var niger, Pseudomonas fluorescens, and Escherichia coli were measured using the combination of a pre-amplifier and a lock-in amplifier. The conductance data were modeled verses the bacterial concentrations. Results indicated that the relationship between the conductance of bacterial suspensions and their concentrations follows a generic model: Y=C 1 + C 2 × e (−X/C 3 ) , where Y is the conductance (S), X is the bacterial concentration (Number/mL: abbreviated to N/mL) for all species tested, and C 1−3 are constants. Gram negative P. fluorescens and E. coli assumed similar conductance curves, which were flatter than that of gram positive B. subtilis var niger. For P. fluorescens and E. coli the culturing technique resulted in higher concentration levels (statistically significant) from 2 to 4 times that measured by the impedance based technology. For B. subtilis var niger, both methods resulted in similar concentration levels. These differences might be due to membrane types, initial culturability and the obtained conductance curves. The impedance based technology here was shown to obtain the bacterial concentration instantly, holding broad promise in realtime monitoring biological agents.

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supporting

confidence: 64%

Development of a novel conductance-based technology for environmental bacterial sensing

Shen

Tan

et al. 2013

Chin. Sci. Bull.

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“…Gottschamel et al [88] developed a disposable microfluidics biochip for multiparameter Candida albicans population measurements, which can monitor Candida albicans growth rates and metabolic activities by simultaneous bioimpedance spectroscopy and amperometric measurements. Zhu et al [92] used fluidic electrodes to fabricate a microfluidics device for detecting bacterial cells in deionized water suspensions with a detection limit of 10 3 cfu·mL −1 . KCl solution was utilized as both sheath flow and fluidic electrodes, and the bacterial suspension was squeezed to form three flowing layers with different conductivities on a microfluidics chip.…”

Section: Microfluidics Techniquesmentioning

confidence: 99%

New Trends in Impedimetric Biosensors for the Detection of Foodborne Pathogenic Bacteria

Wang

Ying

2012

Sensors

228

122

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The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security. In recent years impedimetric biosensors which integrate biological recognition technology and impedance have gained widespread application in the field of bacteria detection. This paper presents an overview on the progress and application of impedimetric biosensors for detection of foodborne pathogenic bacteria, particularly the new trends in the past few years, including the new specific bio-recognition elements such as bacteriophage and lectin, the use of nanomaterials and microfluidics techniques. The applications of these new materials or techniques have provided unprecedented opportunities for the development of high-performance impedance bacteria biosensors. The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element. In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.

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“…Coupled with the physics-based models, in practice, one needs analytical techniques to assess the electrical response of the sample. Particularly, for biological cells, the electrical impedance spectroscopy (EIS) [6] is widely accepted as a label-free, non-invasive and quantitative analytical method that can thoroughly assess the electrical properties of biological cells [7].…”

Section: Introductionmentioning

confidence: 99%

Time-Constant-Domain Spectroscopy: An Impedance-Based Method for Sensing Biological Cells in Suspension

et al. 2021

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Impedance measurement is a common technique to characterize and detect the electrical properties of biological cells. However, to decode the underlying physical processes, it requires complex electrical models alongside prior knowledge of the sample under study. In this work, we introduce an attractive label-free method for sensing biological cells in suspension based on the measurement of electrical impedance and the distribution of relaxation times (DRT) model. The DRT maps impedance data from the frequency-domain to a time-constant-domain spectrum (TCDS) being a useful and robust method for data analysis. We perform impedance measurements in the range from 1 kHz to 1 MHz to obtain the TCDS for sensing mimic samples as well as HeLa cells in suspension. Results show that the TCDS can be seen as an electrical fingerprint for the sample, as it can decode useful information about the composition and structure with high sensitivity and resolution.

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Detection of bacterial cells by impedance spectra via fluidic electrodes in a microfluidic device

Cited by 29 publications

References 31 publications

Development of a novel conductance-based technology for environmental bacterial sensing

Development of a novel conductance-based technology for environmental bacterial sensing

New Trends in Impedimetric Biosensors for the Detection of Foodborne Pathogenic Bacteria

Time-Constant-Domain Spectroscopy: An Impedance-Based Method for Sensing Biological Cells in Suspension

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