Detection of Bacterial Causes of Conjunctivitis Among Neonates and Under five Children and Their Antibiotic Sensitivity Pattern

Shams, Farhana; Shamsuzzaman, SM; Paul, SK; Khan, Tarek Mahbub; Rabin, Nadia; Sarkar, Dipika

doi:10.3329/ssmcj.v30i2.61927

Cited by 1 publication

(1 citation statement)

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“…P. aeruginosa and S. aureus are also implicated in the aetiology of ophthalmia neonatorum [152][153][154][155][156][157], as well as microbial keratitis in adults including in cases of contact lens wear [7,[158][159][160][161][162][163][164][165][166]. Fluoroquinolones have also been used for treatment of ophthalmia neonatorum [143] and microbial keratitis [166][167][168][169].…”

Section: Log Reductionmentioning

confidence: 99%

A novel ex vivo bovine corneal infection and clearance model for Neisseria gonorrhoeae, Staphylococcus aureus, and Pseudomonas aeruginosa

Ukachukwu

Alany

Snyder

2023

Preprint

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Ocular infections caused by bacterial pathogens may damage the cornea and rapidly progress to permanent blindness. Topical application of an ophthalmic formulation is often used to treat corneal infections. The animal models used in many preclinical studies frequently involve expensive in vivo experiments that compromises the corneal epithelium to mimic real life conditions such as during contact lens wear, but these do not consider other instances where infection occurs in intact corneas such as in ophthalmia neonatorum. To develop an ex vivo model of infection, bovine eyes from human food chain waste were processed and the corneas inoculated with Neisseria gonorrhoeae strain NCCP 11945, Staphylococcus aureus strain 6571, and Pseudomonas aeruginosa strain ATCC 15442 for 1 hour, 4 hours, and 6 hours, respectively. Inoculation included intact bovine corneas and those compromised with scalpel, needle, and blot methods. Recovery of N. gonorrhoeae, S. aureus, and P. aeruginosa colonies demonstrated that infection of bovine corneas was achieved with intact and compromised corneas using this model. In addition, corneas inoculated with the bacteria were treated with a suitable antibiotic, demonstrating clearance of the bacterial infections with at least 5 log10 reduction. This model is appropriate for both establishing infection and testing the ability of antimicrobial agents to clear bacterial eye infections. The bovine ex vivo model is reliable, cost-effective, suitable for different bacteria species, and reduces the need for further animal exploitation in laboratory research.

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