Detection of bacterial 16S ribosomal RNA genes for forensic identification of vaginal fluid

Akutsu, Tomoko; Motani, Hisako; Watanabe, Ken; Iwase, Hirotaro; Sakurada, Koichi

doi:10.1016/j.legalmed.2012.01.005

Cited by 59 publications

(40 citation statements)

References 20 publications

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“…However, most abundant genera present in Hou et al's study were also identified abundant in our data, such as Lactobacillus (19.9%), Prevotella (8.51%), Finegoldia (2.73%), Corynebacterium (2.04%), Staphylococcus (1.46%) and Veillonella (1.04%). In addition, some of our relative abundant bacteria had been identified in previous studies which used PCR-based or culture methods to detect bacteria in semen or the male genital tract, such as Lactobacillus [18] , [22] , [29] , Pseudomonas [22] , [29] , Gardnerella [18] , [20] , [30] , [31] , Prevotella [29] , [32] and Haemophilus [22] . It shows the comparability and reliability of our sequencing data.…”

Section: Discussionmentioning

confidence: 99%

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Bacterial Communities in Semen from Men of Infertile Couples: Metagenomic Sequencing Reveals Relationships of Seminal Microbiota to Semen Quality

et al. 2014

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Some previous studies have identified bacteria in semen as being a potential factor in male infertility. However, only few types of bacteria were taken into consideration while using PCR-based or culturing methods. Here we present an analysis approach using next-generation sequencing technology and bioinformatics analysis to investigate the associations between bacterial communities and semen quality. Ninety-six semen samples collected were examined for bacterial communities, measuring seven clinical criteria for semen quality (semen volume, sperm concentration, motility, Kruger's strict morphology, antisperm antibody (IgA), Atypical, and leukocytes). Computer-assisted semen analysis (CASA) was also performed. Results showed that the most abundant genera among all samples were Lactobacillus (19.9%), Pseudomonas (9.85%), Prevotella (8.51%) and Gardnerella (4.21%). The proportion of Lactobacillus and Gardnerella was significantly higher in the normal samples, while that of Prevotella was significantly higher in the low quality samples. Unsupervised clustering analysis demonstrated that the seminal bacterial communities were clustered into three main groups: Lactobacillus, Pseudomonas, and Prevotella predominant group. Remarkably, most normal samples (80.6%) were clustered in Lactobacillus predominant group. The analysis results showed seminal bacteria community types were highly associated with semen health. Lactobacillus might not only be a potential probiotic for semen quality maintenance, but also might be helpful in countering the negative influence of Prevotella and Pseudomonas. In this study, we investigated whole seminal bacterial communities and provided the most comprehensive analysis of the association between bacterial community and semen quality. The study significantly contributes to the current understanding of the etiology of male fertility.

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Section: Discussionmentioning

confidence: 99%

“…Akutsu et al performed PCR-based detection of 16S ribosomal RNA genes for six species and detected Lactobacillus inner and. Gardnerella vaginalis in 28% and 14% of semen samples, respectively [18] . Domes et al determined the incidence of bacteriospermia and found elevated seminal leukocytes (ESL) in a subfertile male population.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Communities in Semen from Men of Infertile Couples: Metagenomic Sequencing Reveals Relationships of Seminal Microbiota to Semen Quality

et al. 2014

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“…65 The microflora of the vagina has been exploited by several authors as an alternative tool to identify vaginal material and is described in the following text. [85][86][87][88][89] Menstrual blood is also a complex fluid composed of varying amounts of circulatory blood, vaginal secretions, microbial communities, and cells associated with the menstrual cycle. It is the remodeling of the endometrium during menstruation that offers potential candidates for identification.…”

Section: Rna-and Dna-based Technologiesmentioning

confidence: 99%

“…[128][129][130][131] The microflora of the vagina has been exploited by several authors as a tool to identify vaginal material. [85][86][87][88] A healthy human vagina is dominated by lactobacilli, and typically Lactobacillus crispatus, L. gasseri, L. jensenii, and/or L. iners are found in women. Usually one species predominates; for example, L. crispatus is prevalent among women in North America, Europe, and Asia.…”

Section: Microbial Community Profilingmentioning

confidence: 99%

Forensic body fluid identification: state of the art

Harbison¹,

Fleming²

2016

RRFMS

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Body fluid identification is a key component in the forensic scientists' tool box and has been carried out both at the crime scene and in the laboratory for many years. Historically, methods relied on (bio) chemical-based tests, many of which lacked specificity. In this review, current technologies for identifying body fluids are described including the use of RNA (mRNA and miRNA), epigenetics, spectroscopic techniques such as Raman spectroscopy and microspectrophotometry, biosensors, and immunochromatographic methods which are outlined alongside their strengths and weaknesses. The potential for new insights into the identification of cells from new technologies such as massively parallel sequencing is explored.

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“…For example, Lactobacillus crispatus, Lactobacillus jensenii, and Atopobium vaginae have been associated with vaginal secretions, while Lactobacillus iners, Lactobacillus gasseri, and Gardnerella vaginalis have been found in other body fluids as well (56)(57)(58). Tissue specific to the vagina could be informative in rape cases.…”

Section: Microbial Forensics and Human Identity Testingmentioning

confidence: 99%

Expansion of Microbial Forensics

2016

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cMicrobial forensics has been defined as the discipline of applying scientific methods to the analysis of evidence related to bioterrorism, biocrimes, hoaxes, or the accidental release of a biological agent or toxin for attribution purposes. Over the past 15 years, technology, particularly massively parallel sequencing, and bioinformatics advances now allow the characterization of microorganisms for a variety of human forensic applications, such as human identification, body fluid characterization, postmortem interval estimation, and biocrimes involving tracking of infectious agents. Thus, microbial forensics should be more broadly described as the discipline of applying scientific methods to the analysis of microbial evidence in criminal and civil cases for investigative purposes.

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Detection of bacterial 16S ribosomal RNA genes for forensic identification of vaginal fluid

Cited by 59 publications

References 20 publications

Bacterial Communities in Semen from Men of Infertile Couples: Metagenomic Sequencing Reveals Relationships of Seminal Microbiota to Semen Quality

Bacterial Communities in Semen from Men of Infertile Couples: Metagenomic Sequencing Reveals Relationships of Seminal Microbiota to Semen Quality

Forensic body fluid identification: state of the art

Expansion of Microbial Forensics

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