Detection of bacteremia by Difco ESP blood culture system

Morello, Josephine A.; Leitch, Cindy; Nitz, S; Dyke, J.W. Van; Andruszewski, M. T.; Maier, G; Landau, William M.; Beard, Mary A.

doi:10.1128/jcm.32.3.811-818.1994

Cited by 39 publications

(21 citation statements)

References 10 publications

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“…Three continuously monitored noninvasive blood culture systems, the BacT/Alert (Organon Teknika, Durham, N.C.) (13), the ESP (Difco Laboratories, Detroit, Mich.) (5), and the BACTEC 9240 (Becton Dickinson Instruments, Sparks, Md.) (6) systems, are in widespread use.…”

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Comparison of the BacT/Alert FAN aerobic and the Difco ESP 80A aerobic bottles for pediatric blood cultures

et al. 1997

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We compared the BacT/Alert system using the aerobic FAN bottle with the ESP system using the 80A aerobic bottle for the detection of pediatric bloodstream pathogens at a children's hospital. From 6,636 blood culture sets complying with the inclusion criteria, 308 pathogens were detected, including 177 that were detected by both systems, 69 that were detected by BacT/Alert FAN only, and 62 that were detected by ESP 80A only (P ‫؍‬ 0.6; not significant). BacT/Alert FAN detected more isolates of Staphylococcus aureus (47 versus 34; P ‫؍‬ 0.02), while ESP 80A detected more episodes of streptococcal and enterococcal infection. BacT/Alert FAN detected more pathogens from patients receiving antibiotic therapy (107 versus 93; P ‫؍‬ 0.04). Of 248 separate episodes of bacteremia or fungemia, 146 were detected by both systems, 56 were detected by ESP 80A only, and 46 were detected by BacT/Alert FAN only (P ‫؍‬ 0.37; not significant). The median times to detection were 13.6 h for ESP 80A and 15.7 h for BacT/Alert FAN (P < 0.001). Both systems were considered easy to operate and were free from significant mechanical difficulties. False-positive or false-negative signals were rare or nonexistent with both systems. We conclude that both systems rapidly detect a broad range of pediatric bloodstream pathogens. BacT/Alert FAN provides better detection of Staphylococcus aureus, especially from patients receiving antibiotics. ESP 80A provides better detection of streptococci and enterococci.

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Comparison of the BacT/Alert FAN aerobic and the Difco ESP 80A aerobic bottles for pediatric blood cultures

et al. 1997

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“…One of the most important functions of the microbiology laboratory is the rapid and accurate detection of microorganisms in the blood. In the last several years, new, automated, continuous-monitoring blood culture systems have been developed, evaluated, and reported in the literature (3)(4)(5)(6)(7)(8). One such system is the BACTEC 9240 (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.…”

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Evaluation of BACTEC 9240 blood culture system by using high-volume aerobic resin media

et al. 1995

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The BACTEC 9240 blood culture system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.) is one of three automated, continuous-monitoring systems that is widely used in clinical laboratories. The BACTEC 9240 was compared with the BACTEC NR 660 for the detection of organisms and bacteremic episodes; time to detection of positive cultures; number of false-positive and false-negative cultures; and time needed to load, process, and perform quality control functions by using high-volume aerobic media. Blood specimens (5,282) were inoculated in equal volumes (5 to 10 ml per bottle) into BACTEC Plus Aerobic/F (9240 system) and BACTEC Plus NR26 (660 system) bottles. Clinically significant isolates were detected in 6.6% of cultures, representing 348 microorganisms and 216 bacteremic episodes. Two hundred forty-eight microorganisms were detected by both systems, 48 by the 9240 only and 52 by the 660 only (P ‫؍‬ not significant). Of the bacteremic episodes, 158 were detected by both systems, 27 by the 9240 only and 31 by the 660 only (P ‫؍‬ not significant). Analysis of data by month revealed equivalent recovery rates for both systems, with the exception of a 30-day period at one study site during which the 660 system detected significantly more microorganisms. Following a proprietary hardware design retrofit of the 9240 instrument, detection rates were again equivalent for the remaining three months at this study site. Positive cultures detected by both systems were detected an average of 4.3 h faster by the 9240 system (21 versus 25.3 h). The numbers of false-positive cultures for the 9240 and 660 systems were 40 (1.0%) and 9 (<1.0%), respectively. The numbers of false-negative cultures were five for the 9240 system and three for the 660 system. The 9240 system required 23 s less technologist time per bottle to operate during the 5-day protocol. In conclusion, the BACTEC 9240 used with high-volume Aerobic/F medium is equivalent to the BACTEC 660 used with high-volume NR26 medium for the detection of microorganisms and bacteremic episodes. In addition, the 9240 system detects positive cultures more rapidly than the 660 system but requires further evaluation to ensure reliability of instrument components.

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“…the first clinical evaluation of ESP, Morello et al(114) compared ESP with the BACTEC NR660 blood culture system. ESP aerobic bottles recovered significantly more Streptococcus pneumoniae and all microorganisms combined, whereas ESP anaerobic bottles recovered significantly more aerobic gram-positive bacteria, Candida spp., and all microorganisms combined.…”

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confidence: 99%