Detection of Babesia divergens using molecular methods in anemic patients in Shandong Province, China

Qi, Chenze; Zhou, Dong; Liu, Jianzhu; Cheng, Ziqiang; Zhang, Li; Wang, Lin; Wang, Zhenyong; Yang, Du-Bao; Wang, Shujing; Chai, Tongjie

doi:10.1007/s00436-011-2382-8

Cited by 52 publications

(29 citation statements)

References 17 publications

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“…Babesiosis, the causative pathogens including B. microti , B. venatorum , and B. divergens in humans, is considered an emerging threat in China, where there are approximately 1.3 billion people at risk of infection (Qi et al, 2011; Jiang et al, 2015; Vannier and Krause, 2015). B. microti was detected in I. persulcatus , H. longicornis , and H. concinna in Fujian, Zhejiang, Henan, and Heilongjiang provinces (Saito-Ito et al, 2008; Sun et al, 2008; Zhou et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

et al. 2016

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Tick-borne diseases are considered as emerging infectious diseases in humans and animals in China. In this study, Ixodes persulcatus (n = 1699), Haemaphysalis concinna (n = 412), Haemaphysalis longicornis (n = 390), Dermacentor nuttalli (n = 253), and Dermacentor silvarum (n = 204) ticks were collected by flagging from northeastern China, and detected for infection with Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. by using nested polymerase chain reaction assays and sequencing analysis. Anaplasma phagocytophilum was detected in all tick species, i.e., I. persulcatus (9.4%), H. longicornis (1.9%), H. concinna (6.5%), D. nuttalli (1.7%), and D. silvarum (2.3%); Anaplasma bovis was detected in H. longicornis (0.3%) and H. concinna (0.2%); Ehrlichia muris was detected in I. persulcatus (2.5%) and H. concinna (0.2%); Candidatus Neoehrlichia mikurensis was only detected in I. persulcatus (0.4%). The Ehrlichia variant (GenBank access number KU921424), closely related to Ehrlichia ewingii, was found in H. longicornis (0.8%) and H. concinna (0.2%). I. persulcatus was infected with Babesia venatorum (1.2%), Babesia microti (0.6%), and Babesia divergens (0.6%). Additionally, four Babesia sequence variants (GenBank access numbers 862303–862306) were detected in I. persulcatus, H. longicornis, and H. concinna, which belonged to the clusters formed by the parasites of dogs, sheep, and cattle (B. gibsoni, B. motasi, and B. crassa). Two Hepatozoon spp. (GenBank access numbers KX016028 and KX016029) associated with hepatozoonosis in Japanese martens were found in the collected ticks (0.1–3.1%). These findings showed the genetic variability of Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. circulating in ticks in northeastern China, highlighting the necessity for further research of these tick-associated pathogens and their role in human and animal diseases.

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Section: Discussionmentioning

confidence: 99%

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

et al. 2016

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“…In addition, some Babesia species can infect both humans and animals, one of which is B. divergens , the primary agent of human babesiosis in Europe (Malandrin et al, 2010). In China, two patients from Shandong Province were confirmed to be infected with B. divergens (Qi et al, 2011), but B. divergens infections in cattle have not been reported in China until now.…”

Section: Introductionmentioning

confidence: 99%

A Molecular Survey of Babesia Species and Detection of a New Babesia Species by DNA Related to B. venatorum from White Yaks in Tianzhu, China

Liu

Guan

et al. 2017

Front. Microbiol.

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Bovine babesiosis is a tick-transmitted disease caused by different species of Babesia. The white yak is a unique yak breed that lives only in Tianzhu in the Tibetan Autonomous County, Gansu Province, in northwestern China. Previous research using the ELISA method has confirmed that the white yak could become infected with B. bigemina. The objective of this study was the molecular detection and identification of Babesia species in white yaks. A total of 409 white yak blood samples were collected from 11 areas of the Tianzhu Tibetan Autonomous County in Northwest China from April to August, 2015. The V4 hypervariable region of Babesia 18S rRNA was amplified from extracted genomic DNA using nested PCR and sequenced. The nearly full-length sequence of 18S rRNA including the V4 region from the newly discovered Babesia was amplified and sequenced with Sanger method. PCR detection and sequencing indicated that 4/409 samples were positive for B. bigemina, 3/409 samples were positive for B. bovis, and 5/409 samples were positive for B. ovata. Additionally, a new Babesia species was found in 4/409 white yaks. A unique sequence of 1,627 bp was obtained from two of the four samples. The sequence was similar to Babesia species Akita (98.5%) found in Ixodes ovatus and B. venatorum (98%) and shared a 98% identity with B. divergens and a 98.1% identity with B. odocoilei. This study provides new data about Babesia infections in white yaks in northwestern China, and a new Babesia species similar to B. venatorum was identified in white yaks for the first time.

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“…Recently, white-tailed deer were indicated as being responsible for reintroducing the tick vector of Cattle Tick Fever in Central Texas and hence the piroplasms responsible for bovine babesiosis ( Babesia bovis and Babesia bigemina ) [11]. Different Theileria and Babesia species were reported in wildlife with high prevalence of infection [5,9,12], some of which are recognized zoonotic pathogens, such as Babesia divergens [13], Babesia divergens -like [14,15], Babesia venatorum (formerly Babesia sp. EU1) [16-18], Babesia microti [2,19,20] and B. microti -like [21,22].…”

Section: Introductionmentioning

confidence: 99%

Piroplasmosis in wildlife: Babesia and Theileria affecting free-ranging ungulates and carnivores in the Italian Alps

et al. 2014

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BackgroundPiroplasmosis are among the most relevant diseases of domestic animals. Babesia is emerging as cause of tick-borne zoonosis worldwide and free-living animals are reservoir hosts of several zoonotic Babesia species. We investigated the epidemiology of Babesia spp. and Theileria spp. in wild ungulates and carnivores from Northern Italy to determine which of these apicomplexan species circulate in wildlife and their prevalence of infection.MethodsPCR amplification of the V4 hyper-variable region of the 18S rDNA of Babesia sp./Theileria sp was carried out on spleen samples of 1036 wild animals: Roe deer Capreolus capreolus (n = 462), Red deer Cervus elaphus (n = 52), Alpine Chamois Rupicapra rupicapra (n = 36), Fallow deer Dama dama (n = 17), Wild boar Sus scrofa (n = 257), Red fox Vulpes vulpes (n = 205) and Wolf Canis lupus (n = 7). Selected positive samples were sequenced to determine the species of amplified Babesia/Theileria DNA.ResultsBabesia/Theileria DNA was found with a mean prevalence of 9.94% (IC95% 8.27-11.91). The only piroplasms found in carnivores was Theileria annae, which was detected in two foxes (0.98%; IC95% 0.27-3.49). Red deer showed the highest prevalence of infection (44.23%; IC95% 31.6-57.66), followed by Alpine chamois (22.22%; IC95% 11.71-38.08), Roe deer (12.55%; IC95% 9.84-15.89), and Wild boar (4.67%; IC95% 2.69-7.98). Genetic analysis identified Babesia capreoli as the most prevalent piroplasmid found in Alpine chamois, Roe deer and Red deer, followed by Babesia bigemina (found in Roe deer, Red deer and Wild boar), and the zoonotic Babesia venatorum (formerly Babesia sp. EU1) isolated from 2 Roe deer. Piroplasmids of the genus Theileria were identified in Wild boar and Red deer.ConclusionsThe present study offers novel insights into the role of wildlife in Babesia/Theileria epidemiology, as well as relevant information on genetic variability of piroplasmids infecting wild ungulates and carnivores.

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Detection of Babesia divergens using molecular methods in anemic patients in Shandong Province, China

Cited by 52 publications

References 17 publications

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

A Molecular Survey of Babesia Species and Detection of a New Babesia Species by DNA Related to B. venatorum from White Yaks in Tianzhu, China

Piroplasmosis in wildlife: Babesia and Theileria affecting free-ranging ungulates and carnivores in the Italian Alps

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