Detection of Babesia and Theileria species infection in cattle from Portugal using a reverse line blotting method

Silva, M.G.; Marques, Patrícia X.; Oliva, Abel

doi:10.1016/j.vetpar.2010.08.038

Cited by 32 publications

(23 citation statements)

References 12 publications

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“…These data are similar to those found by other authors in Italy, Spain, Portugal and Tunisia [13,14,15, 22, 27] and constitute further evidence of the high prevalence of T. buffeli, indicating that it may be the most common piroplasm in the Mediterranean basin. T. buffeli is considered a non-pathogenic or scarcely pathogenic species, although some cases of disease have been reported [9, 29].…”

Section: Discussionsupporting

confidence: 91%

“…B. bovis was found in 1.6% (24/1,500) of the samples, which is in line with reports from Portugal and Spain [13, 27], while slightly higher percentages were observed in Sicily and Tunisia [14, 22]. The abundant presence, in KR5, of Boophilus annulatus ticks, a vector of B. bovis , might explain the higher positive rate of B. bovis in the same area.…”

Section: Discussionsupporting

confidence: 87%

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Retrospective Study of Hemoparasites in Cattle in Southern Italy by Reverse Line Blot Hybridization

Ceci

Iarussi

Greco

et al. 2014

The Journal of Veterinary Medical Science

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Tick-borne diseases are widespread in tropical and temperate regions and are responsible for important economic losses in those areas. In order to assess the presence and prevalence of various pathogens in southern Italy, we retrospectively analyzed cattle blood samples collected for a previous study in 2000 using reverse line blot (RLB) hybridization. The study had been carried out in three regions of southern Italy on 1,500 randomly selected and apparently healthy adult cattle. RLB showed that 43.7% of the cattle were positive for nine different species of hemoparasites with either a single infection or a mixed infection. Theileria buffeli was the most common species found, being present in 27.3% of the animals, followed by Anaplasma marginale in 18.1%, Anaplasma centrale in 13.8%, Babesia bigemina and Anaplasma bovis in 4.2%, Anaplasma phagocytophilum in 1.7%, Babesia bovis in 1.6%, Babesia major in 0.2% and Babesia divergens in 0.1%. Complete blood counts showed different degrees of anemia in 363 animals (24.2%) and of these, 169 were RLB-positive for at least one pathogen. Among the ticks that were collected from the cattle, the following species were identified: Rhipicephalus bursa, Ixodes ricinus, Hyalomma marginatum, Boophilus annulatus, Dermacentor marginatus and Haemaphysalis (sulcata, parva, inermis and punctata). The results obtained confirmed the spread of endemic tick-borne pathogens in the regions studied.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 87%

Retrospective Study of Hemoparasites in Cattle in Southern Italy by Reverse Line Blot Hybridization

Ceci

Iarussi

Greco

et al. 2014

The Journal of Veterinary Medical Science

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“…Nucleic acid-based methods offer a variety of tools for the diagnosis of parasites. Generally, the detection and diagnosis of piroplasmosis consist of molecular methods, which are as following: (1) PCR (Almeria et al 2001;Yin et al 2008); (2) reverse line blotting (RLB) (Almeria et al 2002;Niu et al 2009;Silva et al 2010); (3) real time PCR (Jaffer et al 2010); (4) LAMP Salih et al 2008). None of Sun et al (2008) these methods could be considered better than another.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous detection of piroplasma infections in field Haemaphysalis qinghaiensis ticks by reverse line blotting

et al. 2011

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Piroplasmosis is caused by tick-borne haemoprotozoan parasites in the genera of Theileria and Babesia, in which numbers of agents are highly pathogenic for cattle, sheep and goats. We developed a reverse line blot (RLB) assay for detection and differentiation of four different parasites, in which 18S ribosomal RNA gene sequence was amplified with a set of universal primers specific for all members in the genera of Theileria and Babesia; and the probes were designed on the basis of hypervariable region 4 (V4 region) of 18S rRNA gene. Three Theileria and one Babesia can be detected simultaneously on this system and it was sensitive to detect a parasitemia level between 10(-5) and 10(-8)%. A total of 149 Haemaphysalis qinghaiensis ticks collected from Lintan County of Gannan Tibetan Autonomous Region was tested by RLB. Among these, 136 tick samples were also tested by a nested PCR assay developed previously. After comparison of these results, it showed that more T. luwenshuni was detected in RLB assay, while more T. uilenbergi was detected in H. qinghaiensis ticks by nested PCR. The RLB has shown capability for simultaneous detection of four species of piroplasm in H. qinghaiensis ticks, indicating its usefulness for epidemiological studies of piroplasmosis.

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“…In Portugal, the importance of theileriosis is becoming increasingly recognised (Branco et al, 2010;Silva et al, 2010) and a recent epidemiological study determined an increased prevalence in the south of the country. In a crosssectional study in Southern Portugal, around 30% of cattle were found to be infected within an area that represents around 50% of this country's cattle population .…”

Section: Introductionmentioning

confidence: 99%

Population diversity of Theileria annulata in Portugal

Gomes

Salgueiro

Inácio

et al. 2016

Infection, Genetics and Evolution

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The tick-borne protozoan parasite Theileria annulata causes tropical theileriosis, a severe disease of cattle that occurs across the Mediterranean littoral, the Middle East and Southern Asia. In the Mediterranean region, the disease has long been perceived as being a constraint to livestock production in North Africa and Turkey but was believed to have minimal impact in Southern European countries. It has recently been demonstrated that in Southern Portugal the prevalence of T. annulata is approximately 30%. While the population genetics of the parasite and the multiplicity of infection in the bovine host have been studied in a number of countries, no information is currently available on the composition of the parasite population in Southern Europe or its relationship to populations in bordering regions. A parasite genotyping system, based on micro-and mini-satellite amplification, was used to perform genetic analysis of T. annulata populations from T. annulata infected cattle in twelve farms in Southern Portugal. A diversity of genotypes and a high multiplicity of infection were found, suggesting that the parasite possesses a panmictic population in this region. In comparison with genotypes found in Tunisia and Turkey, parasites from Portugal form a genetically distinct group and show lower genetic diversity.

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Detection of Babesia and Theileria species infection in cattle from Portugal using a reverse line blotting method

Cited by 32 publications

References 12 publications

Retrospective Study of Hemoparasites in Cattle in Southern Italy by Reverse Line Blot Hybridization

Retrospective Study of Hemoparasites in Cattle in Southern Italy by Reverse Line Blot Hybridization

Simultaneous detection of piroplasma infections in field Haemaphysalis qinghaiensis ticks by reverse line blotting

Population diversity of Theileria annulata in Portugal

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