Detection of Autochthonous Zika Virus Transmission in Sincelejo, Colombia

Camacho, Erwin; Paternina-Gómez, Margaret; Blanco, Pedro; Osorio, Jorge E.; Aliota, Matthew T.

doi:10.3201/eid2205.160023

Cited by 39 publications

(21 citation statements)

References 10 publications

(9 reference statements)

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“…Since its introduction into the Americas, ZIKV has spread essentially uncontrolled with at least 54 countries and territories experiencing autochthonous transmission, including the continental US and multiple US territories [12]. In humans, ZIKV infection typically causes a mild and self-limiting illness known as Zika fever, which often is accompanied by maculopapular rash, headache, and myalgia [13,14]. During the current outbreak, a causal relationship between prenatal ZIKV infection and fetal microcephaly, as well as other serious brain anomalies, has been established [15–17].…”

Section: Introductionmentioning

confidence: 99%

Heterologous Protection against Asian Zika Virus Challenge in Rhesus Macaques

et al. 2016

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BackgroundZika virus (ZIKV; Flaviviridae, Flavivirus) was declared a public health emergency of international concern by the World Health Organization (WHO) in February 2016, because of the evidence linking infection with ZIKV to neurological complications, such as Guillain-Barre Syndrome in adults and congenital birth defects including microcephaly in the developing fetus. Because development of a ZIKV vaccine is a top research priority and because the genetic and antigenic variability of many RNA viruses limits the effectiveness of vaccines, assessing whether immunity elicited against one ZIKV strain is sufficient to confer broad protection against all ZIKV strains is critical. Recently, in vitro studies demonstrated that ZIKV likely circulates as a single serotype. Here, we demonstrate that immunity elicited by African lineage ZIKV protects rhesus macaques against subsequent infection with Asian lineage ZIKV.Methodology/Principal FindingsUsing our recently developed rhesus macaque model of ZIKV infection, we report that the prototypical ZIKV strain MR766 productively infects macaques, and that immunity elicited by MR766 protects macaques against heterologous Asian ZIKV. Furthermore, using next generation deep sequencing, we found in vivo restoration of a putative N-linked glycosylation site upon replication in macaques that is absent in numerous MR766 strains that are widely being used by the research community. This reversion highlights the importance of carefully examining the sequence composition of all viral stocks as well as understanding how passage history may alter a virus from its original form.Conclusions/SignificanceAn effective ZIKV vaccine is needed to prevent infection-associated fetal abnormalities. Macaques whose immune responses were primed by infection with East African ZIKV were completely protected from detectable viremia when subsequently rechallenged with heterologous Asian ZIKV. Therefore, these data suggest that immunogen selection is unlikely to adversely affect the breadth of vaccine protection, i.e., any Asian ZIKV immunogen that protects against homologous challenge will likely confer protection against all other Asian ZIKV strains.

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Section: Introductionmentioning

confidence: 99%

Heterologous Protection against Asian Zika Virus Challenge in Rhesus Macaques

et al. 2016

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“…In October 2015, ZIKV infections were confirmed in Colombia in the state of Bolivar (179,195,196) and subsequently spread to other states (30,(197)(198)(199)(200)(201)(202)(203)(204), with an estimate of about 14,000 cases as of early January 2016 (31). In late 2015, autochthonous ZIKV circulation was reported in 12 other countries and territories of the Americas and the Caribbean: Suriname (29,33), Venezuela (26), Guatemala (205), Honduras (206), Mexico (205), Paraguay (207), Panama (206,208) French Guiana (206), El Salvador (37,209), Haiti (210), Puerto Rico (210), and the French Caribbean (Martinique) (206).…”

Section: Emergence Of Zikv In the Americasmentioning

confidence: 99%

“…According to partial sequencing of the M/E-encoding gene, the (196), Puerto Rico (269), and Guatemala (269). All of them showed more than 99% nucleotide identity with the French Polynesian strains (269).…”

Section: Classification and Phylogeny Of Zikvmentioning

confidence: 99%

Zika Virus

2016

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SUMMARYZika virus (ZIKV) is an arthropod-borne virus (arbovirus) in the genusFlavivirusand the familyFlaviviridae. ZIKV was first isolated from a nonhuman primate in 1947 and from mosquitoes in 1948 in Africa, and ZIKV infections in humans were sporadic for half a century before emerging in the Pacific and the Americas. ZIKV is usually transmitted by the bite of infected mosquitoes. The clinical presentation of Zika fever is nonspecific and can be misdiagnosed as other infectious diseases, especially those due to arboviruses such as dengue and chikungunya. ZIKV infection was associated with only mild illness prior to the large French Polynesian outbreak in 2013 and 2014, when severe neurological complications were reported, and the emergence in Brazil of a dramatic increase in severe congenital malformations (microcephaly) suspected to be associated with ZIKV. Laboratory diagnosis of Zika fever relies on virus isolation or detection of ZIKV-specific RNA. Serological diagnosis is complicated by cross-reactivity among members of theFlavivirusgenus. The adaptation of ZIKV to an urban cycle involving humans and domestic mosquito vectors in tropical areas where dengue is endemic suggests that the incidence of ZIKV infections may be underestimated. There is a high potential for ZIKV emergence in urban centers in the tropics that are infested with competent mosquito vectors such asAedes aegyptiandAedes albopictus.

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“…The transmission mode consists of a mosquito biting an infected host, taking in infected blood containing the virus, and further injecting the infectious saliva into the healthy host system. This process ends in a serious infection, resulting in life-threatening pathogenesis and disease progression [1–6]. ZIKV infection has various transmission modes [4, 7–9] and mechanisms including from mother to child; through blood transfusion, bone marrow transplants, or organ transplants; and sexual transmission.…”

Section: Public Health Implications Of Zika Virus and The Need For Zimentioning

confidence: 99%

Electrochemical Biosensors for Early Stage Zika Diagnostics

Kaushik

Tiwari

Jayant

et al. 2017

Trends in Biotechnology

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Health agencies have declared the recent Zika-virus (ZIKV) infection an epidemic and a public health emergency of global concern due to its association with microcephaly and serious neurological disorders. The unavailability of effective drugs, vaccines, and diagnostic tools increases the demand for efficient analytical devices to detect ZIKV infection. However, high costs, longer diagnostic times, and stringent expertise requirements limit the utility of reverse transcriptase-polymerase chain reaction (RT-PCR) methods for rapid diagnostics. Therefore, developing portable, sensitive, selective, and cost-effective sensing systems to detect ZIKV at pM concentrations in biofluids would be a breakthrough in diagnostics and therapeutics. This review highlights the advancements in developing smart sensing strategies to monitor ZIKV progression, with rapid point-of-care (POC) diagnostics as the ultimate aim.

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Detection of Autochthonous Zika Virus Transmission in Sincelejo, Colombia

Cited by 39 publications

References 10 publications

Heterologous Protection against Asian Zika Virus Challenge in Rhesus Macaques

Heterologous Protection against Asian Zika Virus Challenge in Rhesus Macaques

Zika Virus

Electrochemical Biosensors for Early Stage Zika Diagnostics

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