Detection of Antiviral Antibodies with Predetermined Specificity Using Synthetic Peptide-beta-Lactamase Conjugates: Application to Antibodies Specific for the preS Region of the Hepatitis B Virus Envelope Proteins

Neurath, A. R.; Kent, Stephen B. H.; Strick, N.

doi:10.1099/0022-1317-67-3-453

Cited by 25 publications

(2 citation statements)

References 24 publications

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“…It has recently been shown by us that this interac tion occurs not only in vitro [Machida et al, 1984] with glutaraldehyde-treated HSA [Yu et al, 1985] but also in vivo with a not yet identified serum factor , The reported formation of antibodies against HSA during acute and chronic hepa titis suggests that this phenomenon may in duce autoimmune reactions of pathogenetic relevance [Hellstrom et al, 1986], The data on the natural anti-pre-S2 response after hepati tis B infection are contradictory [Hellstrom et al, 1986, Budkowska et al, 1986. The finding of anti-pre-S2 by immunoblot [unpublished data] in late convalescence and in persons who received a pre-S2-containing vaccine [Thomssen et al, 1983] is in agreement with reports [Neurath et al, 1986a] that the major pre-S2 epitopes are also immunogenic in hu mans. In our opinion, the clinical testing of pre-S2-containing vaccines is presently rea sonable if potential autoimmune reactions are carefully monitored.…”

Section: Pre-s2 Antigen As Vaccine Componentsupporting

confidence: 79%

Immunogenicity of the Gene S and Pre-S Domains in Hepatitis B Virions and HBsAg Filaments

Heermann¹,

Kruse²,

Seifer³

et al. 1987

Intervirology

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The three morphological forms (20-nm particles, filaments, virions) of hepatitis B surface antigen (HBsAg) were isolated from serum of chronic virus carriers or from transfected cell lines. BALB/c mice and guinea pigs were immunized with the antigens and the antibody responses against the three antigenic domains of the viral envelope were assayed. The proportion of pre-S1, pre-S2 and gene S antibodies was similar to the molar proportion of the domains in the immunogens. The major gene S and pre-S1 epitopes were conformational, and the major epitopes of the pre-S2 domain were sequential. The immunogenicity of natural and recombinant antigens was identical. The proportion of subtype-specific antibodies was high. The results suggest that recombinant HBsAg filaments containing both subtypes ad and ay may be optimal hepatitis B vaccines.

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Section: Pre-s2 Antigen As Vaccine Componentsupporting

confidence: 79%

Immunogenicity of the Gene S and Pre-S Domains in Hepatitis B Virions and HBsAg Filaments

Heermann¹,

Kruse²,

Seifer³

et al. 1987

Intervirology

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“…In this short review, only peptides as viral antigens will be considered. Although the review focuses on human immunodefi ciency virus (HIV) serology, it should be mentioned that the technique also is effective for the identification of postinfection anti bodies with specificity for, e.g., Epstein-Barr [3], hepatitis B [4] papilloma [5], and respira tory syncytial virus [2], Synthetic peptide antigens offer a number of advantages over other more complex anti gens. There is no need for the handling of infectious virus.…”

mentioning

confidence: 99%

Human Immunodeficiency Virus Antibody Responses Determined by Site-Directed Serology

Norrby¹

1990

Intervirology

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In this brief review, the use of synthetic peptides representing linear antigenic sites in human immunodeficiency virus (HIV) structural proteins for detection of antibodies in sera from HIV-infected individuals is discussed. It has been demonstrated that peptide antigens offer unique advantages for determination of HIV-specific antibodies. In particular, various peptides representing the region of amino acids 580–620 in the transmembranous glycoprotein have been effectively used. Of primary importance is the fact that properly designed site-specific serological tests allow a distinction between type-specific antibodies, a quality not provided by any other currently available test. Furthermore, synthetic peptide antigens allow the design of highly simplified and effectively standardized assays. Hereby, they lend themselves to use for screening purposes or confirmatory testings not only in industrialized countries, but also in developing countries. It is also possible that tests with selected peptides may measure antibodies which have value in predicting the risk for infection in children born to seropositive mothers and for progression of disease in infected individuals.

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Antibodies recognizing human serum albumin are not elicited by immunization with preS2 sequences of the hepatitis B virus envelope protein

Neurath

Strick

Parker

et al. 1988

Journal of Medical Virology

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Antibodies to the preS2 region of the hepatitis B virus (HBV) envelope protein and to human serum albumin (HSA) were allegedly detected at about the same level in sera of humans with acute or chronic hepatitis B [Hellström et al., 1986]. It was claimed that anti-HSA arises as a result of an immune response to the preS2 sequence and that it was involved in hepatocellular damage. Over 100 sera from animals and humans immunized with HBsAg containing preS2 sequences, or with synthetic peptides from the preS1, preS2, and S regions of the HBV env protein were assayed for anti-HSA. The results revealed the following: 1) Immunization with the native preS2 sequence or with unconjugated synthetic peptides derived from that sequence does not result in elicitation of anti-HSA. Therefore the alleged appearance of anti-HSA during hepatitis B cannot be directly related to an anti-preS2-specific immune response. 2) Some synthetic peptides, whether or not they were derived from the preS2 sequence, when linked to certain carriers, but not to others, elicited in rabbits an anti-HSA response, which was markedly lower than the response to the homologous peptide. These anti-HSA antibodies could be separated from anti-preS2-specific antibodies by affinity chromatography and did not recognize the synthetic peptide used for immunization. The use in active immunoprophylaxis of hepatitis B of unconjugated peptides from the preS2 sequence with proven high immunogenicity will avoid carrier/linker-mediated induction of antibodies not relevant to protection against HBV.

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Detection of Antiviral Antibodies with Predetermined Specificity Using Synthetic Peptide-beta-Lactamase Conjugates: Application to Antibodies Specific for the preS Region of the Hepatitis B Virus Envelope Proteins

Cited by 25 publications

References 24 publications

Immunogenicity of the Gene S and Pre-S Domains in Hepatitis B Virions and HBsAg Filaments

Immunogenicity of the Gene S and Pre-S Domains in Hepatitis B Virions and HBsAg Filaments

Human Immunodeficiency Virus Antibody Responses Determined by Site-Directed Serology

Antibodies recognizing human serum albumin are not elicited by immunization with preS2 sequences of the hepatitis B virus envelope protein

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