Detection of antinuclear antibodies by the Inno‐Lia ANA update test in canine systemic rheumatic disease

Hansson-Hamlin, Helene; Rönnelid, Johan

doi:10.1111/j.1939-165x.2009.00193.x

Cited by 10 publications

(11 citation statements)

References 23 publications

(31 reference statements)

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“…It may also be due to presence of antibodies other than those being tested resulting in negative ANA blot in spite of positive ANA screen. [26][27][28] Our study has certain limitations. Not all antibodies were tested which may cause autoimmune diseases as also that many non AIDs may also cause ANA positivity.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of antinuclear antibodies in central Madhya Pradesh- A prospective study

Hawaldar¹,

Sodani²

2020

IJMR

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Introduction: Autoimmune diseases (AID) are a manifestation of a self damaging immune response of the body to its own antigen. In any suspected AID, the first test to be done is ANA detection by indirect immunofluoresence using HeP 2 cells due to its sensitivity. A positive ANA test is further evaluated by performing immunoblot test to detect specific antigens causing autoimmunity. The present study was undertaken to study the prevalence of antinuclear antibodies in patient population of Central Madhya Pradesh as well as to study the prevalence of disease specific antigens. Materials and Methods: A total of 650 patients of all ages and both sexes coming to our diagnostic centre between January to June 2018 for ANA testing were included in the study. Serum ANA was determined by indirect immunofluorescence (IIFA) using Hep 2 cell lines (Euroimmun, Germany). A positive result by IIFA was further tested by immunoblot method (Euroimmun, Germany) when requested by the clinician. Result: Out of 650 patients ANA screen was positive in 280 (43.08%) patients and negative in 209(32.15%) patients. ANA blot was requested in 161 patients (57.5%) of the total 280 ANA screen positive patients. Out of the 161 patients, 69 had a positive Immunoblot test for one or more antigens. Total 53.69% patients were positive by both ANA screen and immunoblot test. The total coincidence rate was 44.5% between ANA screen and ANA blot test with a positive coincidence rate of 81.6% and negative coincidence rate of 79.1%. Conclusion: ANA IIF is considered to be the gold standard screening test for detecting autoantibodies and ANA blot is done as a confirmatory test to detect specific autoantibodies against antigens causing autoimmunity. The combination of ANA IIF and ANA blot is an effective method to diagnose autoimmune diseases. If used alone, a possibility of missing a diagnosis is high and so a combinbation of these two tests is of great benefit to both the clinicians as well as laboratorians.

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Section: Discussionmentioning

confidence: 99%

Prevalence of antinuclear antibodies in central Madhya Pradesh- A prospective study

Hawaldar¹,

Sodani²

2020

IJMR

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“…Several autoantigens have been previously identified in dogs by different approaches 9 , 21 , 22 , 38 , 46 – 50 . Here, we report the use of human protein arrays for the screening of canine autoantigens.…”

Section: Discussionmentioning

confidence: 99%

ILF2 and ILF3 are autoantigens in canine systemic autoimmune disease

Bremer

Landegren

Sjöberg

et al. 2018

Sci Rep

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Dogs can spontaneously develop complex systemic autoimmune disorders, with similarities to human autoimmune disease. Autoantibodies directed at self-antigens are a key feature of these autoimmune diseases. Here we report the identification of interleukin enhancer-binding factors 2 and 3 (ILF2 and ILF3) as autoantigens in canine immune-mediated rheumatic disease. The ILF2 autoantibodies were discovered in a small, selected canine cohort through the use of human protein arrays; a method not previously described in dogs. Subsequently, ILF3 autoantibodies were also identified in the same cohort. The results were validated with an independent method in a larger cohort of dogs. ILF2 and ILF3 autoantibodies were found exclusively, and at a high frequency, in dogs that showed a speckled pattern of antinuclear antibodies on immunofluorescence. ILF2 and ILF3 autoantibodies were also found at low frequency in human patients with SLE and Sjögren’s syndrome. These autoantibodies have the potential to be used as diagnostic biomarkers for canine, and possibly also human, autoimmune disease.

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“…An autoimmune disease is a condition in which tissue injury is caused by an abnormal T cell or antibody reaction to self. The modified Witebsky's postulates 19,20 characterize the different types of experimental criteria Line blot analysis of antinuclear antibodies in 10 controls (1-10) and 10 DH cases (11)(12)(13)(14)(15)(16)(17)(18)(19)(20). Altogether 4 cases (3 of 10 subclinical and 1 of 10 clinical) showed antihistone reactivity in the line blot.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the autoantigens, 1 control line is present on each strip. We employed the test for antibodies in dogs as previously described and used the diluents, buffers, and color developers provided by the manufacturer. Nine SDH (7 females and 2 males) and 1 female CDH Doberman were included in the case group and 10 healthy Dobermans (6 females and 4 males) in the control group.…”

Section: Methodsmentioning

confidence: 99%

Antihistone Autoantibodies in Dobermans With Hepatitis

Dyggve

Meri

Spillmann

et al. 2017

Veterinary Internal Medicne

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BackgroundImmune system involvement is suggested as an underlying cause for Doberman hepatitis (DH) based on female predisposition, lymphocyte infiltration, abnormal hepatocyte expression of major histocompatibility complex class II antigens, and homozygosity for dog leukocyte antigen DRB1*00601.ObjectiveTo measure serum antinuclear antibodies (ANA) and serum antihistone antibodies (AHA) in Dobermans with hepatitis. To determine whether increased serum ANA or serum AHA could be used to support the diagnosis of Doberman hepatitis (DH).AnimalsPrivately owned 25 subclinically and 13 clinically affected DH Dobermans and 17 healthy control Dobermans.MethodsCase–control study. Indirect immunofluorescence (IIF) microscopy and line blot tests were employed for the ANA pilot studies and an enzyme‐linked immunosorbent assay (ELISA) assay for detection of IgG AHA.ResultsIndirect immunofluorescence revealed ANA‐positive cases, and line blot showed AHA reactivity. In ELISA, importantly increased concentrations of AHA were found in 92% (23/25) of dogs in the subclinical stage and 84.6% (11 of 13) of dogs in the clinical stage of DH compared with no control dogs (0/17) (P < 0.0005). The mean AHA absorbance values of the blood samples obtained from the 25 subclinical DH dogs (1.36 ± 0.60, mean ± SD) and the 13 clinically affected dogs (1.46 ± 0.49) were significantly higher than in 17 control dogs (0.51 ± 0.18; P < 0.0001).Conclusions and Clinical ImportanceAs the presence of AHA indicates autoimmune activity, our results favor an autoimmune background as one cause for DH. Antihistone antibody could represent a novel means for screening Dobermans with increased serum alanine transaminase concentrations and suspicion of DH.

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Detection of antinuclear antibodies by the Inno‐Lia ANA update test in canine systemic rheumatic disease

Cited by 10 publications

References 23 publications

Prevalence of antinuclear antibodies in central Madhya Pradesh- A prospective study

Prevalence of antinuclear antibodies in central Madhya Pradesh- A prospective study

ILF2 and ILF3 are autoantigens in canine systemic autoimmune disease

Antihistone Autoantibodies in Dobermans With Hepatitis

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