Detection of antimicrobial resistance genes of Helicobacter pylori strains to clarithromycin, metronidazole, amoxicillin and tetracycline among Egyptian patients

Diab, Manal; El‐Shenawy, A. I.; El-Ghannam, Maged; Salem, Dalia; Abdelnasser, Moustafa; Shaheen, Mohamed; Abdel-Hady, Mahmoud; El-Sherbini, Effat; Saber, Mohamed

doi:10.1016/j.ejmhg.2018.01.004

Cited by 16 publications

(12 citation statements)

References 42 publications

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“…The current outcome was not in accordance with a past reports in Egyptian investigations Alhammad et al, [25] , Zaki et al [21], and Diab et al, [20] who recorded that obstruction rates to clarithromycin were (26%, 22.4% and 6.7%) individually by utilizing constant PCR for discovery of 23S rRNA.…”

Section: Discussioncontrasting

confidence: 91%

“…Among the examined H. pylori gastric biopsy examples, we detailed impressive phenotypically safe arguments against Metronidazole, Amoxicillin (AMX) and antibiotic medication which were (28%, 20% and 4%) individually utilizing plate dissemination technique. Likewise,( Diab et al, [20] . reported extensive obstruction against metronidazole (25%) and amoxicillin (18.3%) utilizing traditional PCR test followed by sequencing of DNA parts of rdxA and Pbp1A qualities individually and little protection from antibiotic medication utilizing ongoing PCR for recognition of 16S rRNA.…”

Section: Discussionmentioning

confidence: 96%

“…These outcomes were predictable with information gotten from other Egyptian investigation as Alhammad et al, [25] who detailed that, transformation site of 23S rRNA related with clarithromycin opposition was resolved as A2143G in the lion's share (77%) of H. pylori strains . Notwithstanding that, Vala et al, [32].and Diab et al, [20].detected A2143G point transformation in 100% of H. pylori strains recuperated from their examined patients in Iran and Egypt, individually. Additionally, Acosta et al, [33].announced that A2143G transformation was more incessant than A2142G change.…”

Section: Discussionmentioning

confidence: 96%

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Determination of Antibiotic Resistance and Relevance of 23S Rrna Gene in Helicobacter Pylori with Clarithromycin Resistance in Chronic Gastritis

Abdelsami

Fari

Abbas

et al. 2020

Benha Journal of Applied Sciences

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Anti-toxin opposition of Helicobacter pylori (H. pylori) to treatment is going up. The clarithromycin obstruction is the serious issue of treatment disappointment in H. pylori disease .There is a basic need to decide the ebb and flow paces of such obstruction before authoritative treatment.We intended to decide antimicrobial opposition paces of H. pylori and impacts of point transformations during the 23S rRNA quality in H. pylori on clarithromycin opposition among the Egyptian populace, as a necessary advance prior to starting treatment.Fifty patients with H. pyloripositive ongoing gastritis were analyzed. H. pylori was distinguished by H.pylori stool antigen and microbiological refined of gastric biopsy . Absolute DNA was extricated from gastric biopsy examples and A2142G and A2143G transformations were recognized by PCR-limitation section length polymorphism (PCR-RFLP).H. pylori obstruction rates to clarithromycin , metronidazole, amoxicillin, and antibiotic medication were 54%, 28%,20% and 4% respectively.Additionally, for clarithromycin point changes in 23S rRNA types A2142G and A2143G of H. pylori were surveyed by PCR-RFLP measure, freak qualities were discovered to be 92.6 % of clarithromycin obstruction. A2143G change was recognized in 74.1% of tests and A2142G transformation in 18.5%. There was a solid relationship among MICs and point transformations in 23S rRNA gene.A2143G change was unmistakable . H. pylori conveying A2142G change indicated significantiy more elevated levels of MIC values for Clarithromycin anti-microbial. PCR-RFLP has uncovered a solid test permitting a fast identification of clarithromycin-obstruction. This is helpful in our nation where there is high pervasiveness of clarithromycin-obstruction prior to picking ideal treatment for H. pylori destruction.

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Section: Discussioncontrasting

confidence: 91%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 96%

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Determination of Antibiotic Resistance and Relevance of 23S Rrna Gene in Helicobacter Pylori with Clarithromycin Resistance in Chronic Gastritis

Abdelsami

Fari

Abbas

et al. 2020

Benha Journal of Applied Sciences

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“…However, their detection has been done in H. pylori bacteria recovered from human clinical specimens. 14,48,51 The final part of our survey focused on the genotyping of vacA, cagA, cagE, iceA, oipA, and babA alleles of the H. pylori bacteria. We also originated that vacA s1a, s2, m1a, and m2, and iceA1 and cagA, s1am1a, s2m1a, s1am2, s2m2, and s1a/cagA-/iceA1/oipA-/cagE-/babA-, m1a/cagA-/ iceA1/oipA-/cagE-/babA-, s2/cagA-/iceA1/oipA-/cagE-/ babA-, s1a/cagA+/iceA1/oipA+/cagE-/babA-, m1a/cagA +/iceA1/oipA+/cagE+/babA-, m1a/cagA+/iceA1/oipA +/cagE-/babA-, m2/cagA-/iceA1/oipA-/cagE-/babA-, s1a/ cagA+/iceA1/oipA+/cagE+/babA-, s1a/cagA-/iceA1/oipA-/ cagE+/babA-, s1a/cagA-/iceA1/oipA+/cagE-/babA-, s1b/ cagA-/iceA1/oipA-/cagE-/babA-, m1a/cagA-/iceA1/oipA-/ cagE+/babA-, m1a/cagA-/iceA1/oipA+/cagE-/babA-, m1b/ cagA-/iceA1/oipA-/cagE-/babA-, s1a/cagA+/iceA1/oipA-/ cagE+/babA-, s2/cagA+/iceA1/oipA+/cagE-/babA-, s2/ cagA-/iceA1/oipA-/cagE+/babA-, s2/cagA-/iceA1/oipA+/ca gE-/babA-, m1a/cagA+/iceA1/oipA-/cagE-/babA+, and m1a/cagA+/iceA1/oipA-/cagE+/babA-were the most generally perceived genotypes amongst the H. pylori bacteria.…”

Section: Combined Genotyping Patterns Distribution* (%)mentioning

confidence: 99%

Phenotypic and Genotypic Assessment of Antibiotic Resistance and Genotyping of vacA, cagA, iceA, oipA, cagE, and babA2 Alleles of Helicobacter pylori Bacteria Isolated from Raw Meat

Mashak¹,

Jafariaskari²,

Alavi³

et al. 2020

IDR

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Background: Foodstuffs with animal origins, particularly meat, are likely reservoirs of Helicobacter pylori. Purpose: An existing survey was accompanied to assess phenotypic and genotypic profiles of antibiotic resistance and genotyping of vacA, cagA, cagE, iceA, oipA, and babA2 alleles amongst the H. pylori bacteria recovered from raw meat. Methods: Six-hundred raw meat samples were collected and cultured. H. pylori isolates were tested using disk diffusion and PCR identification of antibiotic resistance genes and genotyping. Results: Fifty-two out of 600 (8.66%) raw meat samples were contaminated with H. pylori. Raw ovine meat (13.07%) had the uppermost contamination. H. pylori bacteria displayed the uppermost incidence of resistance toward tetracycline (82.69%), erythromycin (80.76%), trimethoprim (65.38%), levofloxacin (63.46%), and amoxicillin (63.46%). All H. pylori bacteria had at least resistance toward one antibiotic, even though incidence of resistance toward more than eight antibiotics was 28.84%. Total distribution of rdxA, pbp1A, gyrA, and cla antibiotic resistance genes were 59.61%, 51.92%, 69.23%, and 65.38%, respectively. VacA s1a (84.61%), s2 (76.92%), m1a (50%), m2 (39.13%), iceA1 (38.46%), and cagA (55.76%) were the most generally perceived alleles. S1am1a (63.46%), s2m1a (53.84%), s1am2 (51.92%), and s2m2 (42.30%) were the most generally perceived genotyping patterns. Frequency of cagA-, oipA-, and babA2-genotypes were 44.23%, 73.07%, and 80.76%, respectively. A total of 196 combined genotyping patterns were also perceived. Conclusion: The role of raw meat, particularly ovine meat, in transmission of virulent and resistant H. pylori bacteria was determined. VacA and cagA genotypes had the higher incidence. CagE-, babA2-, and oipA-H. pylori bacteria had the higher distribution. Supplementary surveys are compulsory to originate momentous relations between distribution of genotypes, antibiotic resistance, and antibiotic resistance genes.

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“…shows that there are no significant differences between the studied groups as regards demographic data (age, sex, body mass index, smoking, and residence).The mean age was29.70 years in group A, 30.66 in group Band 30.32 in group C. The mean body mass index (BMI) was 26.93 in group A, 26.42 in group B, and 26.80 in group C.…”

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confidence: 83%

The Assessment of Gastric Antral Wall Thickness in Helicobacter pylori gastritis by Abdominal Ultrasonography, Case-Control Study (2016-2018)

Zaher

Refaey

Dawod

et al. 2020

Afro-Egyptian Journal of Infectious and Endemic Diseases

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Background and Aim: Abdominal ultrasonography is effective in the visualization of gastric wall layers and measuring its thickness. The study aimed to assess gastric antral wall thickness in patients with H. pylori gastritis by abdominal ultrasonography and to study its predictive value in detecting H. pylori gastritis. Materials and Methods:The study included ninety adult individuals, sixty of them had dyspepsia and/or upper abdominal pain and histologically confirmed gastritis, were distributed equally according to the H. pylori infection status into group A (H. pylori gastritis) or B (non-H. pylori gastritis), while, group C included thirty asymptomatic participants with negative H. pylori screening. The participants were subjected to abdominal sonography for measuring the antral wall thickness (AWT), mucosal wall thickness (MLT) and mucosal-to-antral wall thickness ratio (MLT/AWT ratio). Results:The AWT, MLT and MLT/AWT ratio were significantly greater in H. pylori gastritis group (5.65 ± 0.58, 3.02 ± 0.43, 0.53 ± 0.04 respectively) than non-H. pylori gastritis group (4.57± 0.82, 2.07± 0.41, 0.45 ± 0.02 respectively) and control group (3.93 ± 0.52, 1.49 ± 0.2, 20.37± 0.03 respectively). The optimal cut off values of AWT, MLT and MLT/AWT ratio for detecting the of H. pylori infection among symptomatic gastritis patients were > 4.94 mm, > 2.46 mm and > 0.48 mm respectively. Conclusion:Ultrasonography of the gastric antrum is considered beneficial in evaluating patients with presumed gastritis. The increase of AWT > 4.94 mm, MLT > 2.46 mm, and MLT/AWT ratio > 0.48 mm in patients with gastritis is suggestive of H. pylori infection.

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Detection of antimicrobial resistance genes of Helicobacter pylori strains to clarithromycin, metronidazole, amoxicillin and tetracycline among Egyptian patients

Cited by 16 publications

References 42 publications

Determination of Antibiotic Resistance and Relevance of 23S Rrna Gene in Helicobacter Pylori with Clarithromycin Resistance in Chronic Gastritis

Determination of Antibiotic Resistance and Relevance of 23S Rrna Gene in Helicobacter Pylori with Clarithromycin Resistance in Chronic Gastritis

<p>Phenotypic and Genotypic Assessment of Antibiotic Resistance and Genotyping of <em>vacA</em>, <em>cagA</em>, <em>iceA</em>, <em>oipA</em>, <em>cagE</em>, and <em>babA2</em> Alleles of <em>Helicobacter pylori</em> Bacteria Isolated from Raw Meat</p>

The Assessment of Gastric Antral Wall Thickness in Helicobacter pylori gastritis by Abdominal Ultrasonography, Case-Control Study (2016-2018)

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