Detection of Antimicrobial Resistance and Extended-Spectrum β-Lactamase Production in Klebsiella Pneumoniae Strains from Infected Neonates

Abstract

The present study was designed to determine the antimicrobial resistance and extended-spectrum beta-lactamase (ESBL) activities of Klebsiella pneumoniae strains isolated from the neonatal intensive care unit of Atatürk University Hospital, Erzurum, Turkey. Antibiotic susceptibility of 40 isolates was detected by the standard disk diffusion method according to the National Committee for Clinical Laboratory Standards Guidelines. The double-disk synergy method was used to determine ESBL activity, which is associated with resistance to beta-lactam antibiotics. Twenty-four (60%) of 40 K. pneumoniae strains were found to produce ESBL. Of the antibiotics tested, meropenem was found to be the most effective (100%), and ampicillin the least effective (0%). With the increasing incidence of antimicrobial resistance, which poses a clinically significant risk to vulnerable patients, it is important that clinical microbiology laboratories have accurate and timely information concerning the strains of bacteria present to enable them to predict which antibiotics are likely to be effective in treating the infections they may cause.

Antibiotic Susceptibility Pattern of ESβL Producing Klebsiella pneumoniae Isolated from Urine Samples of Pregnant Women in Karnataka

“…Carbapenems are the drugs of choice for many infections caused by Gram positive and Gram negative bacteria and were found to be the most effective antibiotics, and our study revealed 100% susceptible whereas, consistent rise was observed with other studies [23]. Maximum isolates of K.pneumoniae (75.6%) were resistance to ampicillin as comparable with other studies [24]. Cephalosporins, particularly second and third generation have been used to treat Klebsiella pneumoniae infections [25].…”

Healthcare associated infections caused by plasmid-encoded blaKPC and blaNDM strains of Klebsiella pneumoniae in Ibadan, Nigeria

“…In this study, K. pneumoniae isolates demonstrated multiple resistance to the antibiotics tested, which agrees with worldwide reports of increase resistance to antibiotics among Klebsiella spp [19]. Resistance to ampicillin was 100%, and this is similar to the reports of other studies that recorded such high resistance rate [20][21][22][23][24]. The cephalosporins, particularly the second and third generations, have been employed as the drug of choice for treating infections caused by the family enterobacteriaceae including Klebsiella infections [25].…”

Pyogenic and amebic liver abscesses

“…Ampicillin covers enterococci and metronidazole covers anaerobes as well as Entamoeba species. However, Klebsiella species are highly resistant to ampicillin, and β-lactamase inhibitor should be considered [12]. Cefoxitin can be substituted for ampicillin but will not cover enterococci.…”