Detection of antigens on filarial larvae by means of immune adherence

Nelson, Margaret; Nelson, D. S.; Zaman, V.

doi:10.1007/bf02145889

Cited by 5 publications

(3 citation statements)

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“…These results may be compared with the limited examples of cross protection between certain filariae (WONG, 1974;OOTHUMAN et al, 1979;STOREY & AL-MUKHTAR, 1982) but contrast with the specific immunity more generally attributed to animals immunized with developing larvae or blood dwelling mff (NELSON et al, 1971;WONG et a/., 1973;WEISS & TANNER, 1979;KAZURA & DAVIS, 1982). However, there is now good evidence for the sharing of antigenic determinants exposed on the surface of different stages and species of filariae (FORSYTH et al, 1981;MAIZELS et al, 1982) which may ultimately lead to the identification of components involved in modulation of host-parasite relationships.…”

Section: Discussionmentioning

confidence: 97%

Immunity toOnchocerca lienalismicrofilariae in mice

1985

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The model of Onchocerca lienalis microfilariae (mff) injected into CBA and T.O. strains of mice has been used to examine immunity to skin-dwelling microfilariae following exposure to a range of species oF nelmmms Mice which had received a primary infection with O. cervicalis mff were significantly resistant to challenge with O. lienalis mfT(58% reduction relative to challenge controls). Immunization with the uterine contents (eggs and mff) of O. lienalis, O. gutturosa or O. volvulus conferred equivalent levels of protection agains challenge with O. lienalis mff (66 to 75%). Similar results were obtained with immunizations in mice that employed either fresh or freeze-killed eggs of O. gutturosa. Significant reductions in the recoveries of O. lienalis mff were also demonstrated following the intraperitoneal implantation of adult male worms of O. gutturosa (30 to 52%), the adults of either sex of Dipetalonema viteae (60%), or after infection witn Trichinella spiralis (27 to 81%). Infections with the trematode, Schistosoma mansoni, had a negligible effect on mff recoveries. It is concluded that partial resistance in mice to Onchocerca mff may be stimulated by factors, yet to be determined, that are neither stage nor species-specific.

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Section: Discussionmentioning

confidence: 97%

Immunity toOnchocerca lienalismicrofilariae in mice

1985

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“…Stage-specific surface antigens on filarial worms have been indicated by a number of experimental studies. Nelson, Nelson & Zaman (1971) showed that rabbit antibody to Breinlia sergenti infective larvae does not mediate adherence…”

Section: Antigenic Sharing Between Stages In the Life-cycle Of Brugiamentioning

confidence: 99%

Cross-reactive surface antigens on three stages ofBrugia malayi,B. pahangiandB. timori

et al. 1983

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Surface antigens of three stages of three species of the filarial nematode genus Brugia have been analysed by radio-iodination and immunoprecipitation. These surface antigens have been shown to be characteristic for each stage by polyacrylamide gel electrophoresis. For example, infective larvae and adult worms have relatively complex patterns while microfilariae have few bands which are not found when other stages are radio-isotope labelled by the same technique. The surface antigens of Brugia malayi, B. timori and B. pahangi adult worms are all closely homologous, as are the surface antigens of infective larvae of the same three species, and of microfilariae of B. malayi and B. pahangi. Immunoprecipitation revealed that antibody raised in mice against one stage or species reacted with surface antigens from other stages and species. For example, sera raised against B. pahangi male adults reacted strongly with surface antigens from all three species. This cross-reactivity was dominant despite the apparent stage-specificity of the surface pattern seen on SDS-PAGE analysis. Moreover, in cross-immunization experiments, infective larvae were able to stimulate a secondary antibody response in mice previously primed with microfilarial surface antigens. The major microfilarial surface antigens (of mol. wt 65-70 000 Daltons) were recognized by serum antibody from microfilariae-, infective larvae- or adult-infected animals. Thus, although the dominant antigens from each stage are of different molecular weight, cross-reactions with stage-specific antisera suggest that there must be shared epitopes on Brugia surface antigens from each stage. Such shared antigenic determinants dominate the immune response, although other evidence, including the differences in molecular weight, indicates the existence of stage- and species-specific components.

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“…A preliminary indication of the stage-and speciesspecificity of anticuticular antibodies was obtained in an immune adherence assay with sera from rabbits immunized with Breinlia sergenti L3: there was reactivity only against L3, not against mf or adult worms of the same species and not against B. pahangi or B. maluyi L3 (NELSON et al, 1971).…”

Section: Tran~acy~nsmentioning

confidence: 99%