Detection of Antigens of Larval Taenia solium in the Cerebrospinal Fluid of Patients with the Use of HPLC and ELISA

Abstract

The cerebrospinal fluid (CSF) obtained from patients suspected of having neurocysticerosis (79 samples), as well as from control patients (without neurological symptoms), was separated using a high performance liquid chromatography gel filtration column. During the chromatographic separation, the eluted fractions were collected separately according to distinctive peaks. The elution characteristics of CSF components were identified by aligning more than 100 chromatograms and 6 distinctive peaks, eluting in consistent positions. Samples of each peak were tested in an enzyme-linked immunosorbent assay (ELISA) for the presence of larval antigens. Forty-four of the suspected 79 cases were found to have larval antigens in their CSF and these antigens were detected in peak no. 2, the mean of which is approximately 110,000 molecular weight. Also, in some cases, larval antigens were found in peak no. 1; however, we were able to detect them in only 23 CSF samples out of 44 CSF samples in which larval antigens were present in peak no. 2. Nine of these 23 CSF samples (derived from 79 patients in which neurocysticercosis was suspected) were later confirmed by histopathology. Values of ELISA readings of 5 CSF samples obtained from control patients (0.054 +/- 0.064) were considered negative. Thus, in 44 of 79 CSF samples from patients suspected of having neurocysticercosis, the ELISA values were highly positive (0.551 +/- 0.293). The remaining 35 CSF samples gave ELISA readings of 0.092 +/- 0.062, which were not significantly different from values obtained with CSF of control patients.(ABSTRACT TRUNCATED AT 250 WORDS)

Detection of antigen B of Cysticercus cellulosae in cerebrospinal fluid for the diagnosis of human neurocysticercosis

“…However, there was no difference in the pattern of the CSF elute between the NCC patients, patients with other neurological disorders and controls. This is in accordance with a previous study involving separation of CSF by HPLC (Choromanski et al. 1990).…”

“…The CSF samples were analysed by gel filtration chromatography using a HPLC system (Waters Associat Ltd, Milford, MA, USA) basically as described by Choromanski et al. (1990).…”

“…1989; Katti & Chandramukhi 1991). Chromatographic separation of CSF by high performance liquid chromatography (HPLC) and antibody detection in the various fractions have been evaluated and shown to be sensitive and specific (Choromanski et al. 1990).…”

Immunological and molecular diagnosis of cysticercosis

“…Also, only a fraction of all antigens present in the cyst fluid can be detected in the patient's CSF. 131,132 Circulating antigen can also be detected in serum, as initially demonstrated for T. saginata cysticercosis in cattle 133 and later in human samples. 134 Monoclonal antibody (MoAb)-based antigen detection greatly improved the performance of these assays.…”

Cysticercus Antigens in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis

“…The detection of antigens released by the parasite may be useful (5,12,29,33), since it would expand the diagnostic perspectives, considering that antigens, mainly excretory and secretory antigens, appear before the production of antibodies. However, techniques for the detection of antigens require better evaluation and are still not routinely available in the laboratory.…”