Detection of antibody to Staphylococcus aureus teichoic acid by enzyme-linked immunosorbent assay

Yamada, J K; Inderlied, Clark B.; Porschen, Richard K.

doi:10.1128/jcm.17.5.898-905.1983

Cited by 12 publications

(7 citation statements)

References 9 publications

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“…The results presented in this paper are in agreement with the findings of other authors who found the measurement of R-TA antibodies by CIE (3,6,10,13) or ELISA (5,16) useful in both the diagnosis and management of serious S. aureus infections.…”

Section: Discussionsupporting

confidence: 92%

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Comparison of a new enzyme-linked immunosorbent assay method with counterimmunoelectrophoresis for detection of teichoic acid antibodies in sera from patients with Staphylococcus aureus infections

Herzog¹,

Wood²,

Noel³

et al. 1984

J Clin Microbiol

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Ribitol-teichoic acid antibodies were measured by a new enzyme-linked immunosorbent assay (ELISA) and by counterimmunoelectrophoresis in serum samples from 47 patients with serious Staphylococcus aureus infections, 63 infected patients, and 177 healthy controls. The same antigen was used for both tests. The group of patients with S. aureus endocarditis (6 patients) had significantly higher ELISA readings than the patients with other deep-seated infections (26 patients) or with an uncomplicated S. aureus bacteremia (15 patients). The patients with other serious gram-positive (40 patients) or gram-negative (23 patients) infections did not differ from the healthy control group. There were only three (7.5%) low-level crossreactions among the infections caused by gram-positive organisms other than S. aureus. Of 46 initially ribitol-teichoic acid antibody-negative patients followed up for 2 weeks or more, only those developing a serious S. aureus infection showed a significant rise of the ELISA reading. There was a good correlation between ELISA and counterimmunoelectrophoresis. Both tests could be useful in the diagnosis and the management of complicated S. aureus infections. The ELISA method is, however, more sensitive and usually reflects the antibody rise after an infection earlier than does counterimmunoelectrophoresis.

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Section: Discussionsupporting

confidence: 92%

“…Both techniques have limitations, and a better technique is badly needed (12). Recently, several enzyme-linked immunosorbent assay (ELISA) methods have been published, measuring antibodies against either staphylococcal R-TA (5,16) or peptidoglycan (14 This report gives details of a new ELISA for R-TA antibodies that has a number of advantages over CIE.…”

mentioning

confidence: 99%

Comparison of a new enzyme-linked immunosorbent assay method with counterimmunoelectrophoresis for detection of teichoic acid antibodies in sera from patients with Staphylococcus aureus infections

Herzog¹,

Wood²,

Noel³

et al. 1984

J Clin Microbiol

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“…A large number of serological assays for the diagnosis of serious S. aureus infections have been presented during the last decade most often using sensitive methods like RIA and ELISA. The cell wall antigens teichoic acid and peptidoglycan have been used most frequently (1, 3,10,12,14,16,17,18,19,21). A major problem is the lack of standardization in purifying the antigens.…”

Section: Discussionmentioning

confidence: 99%

Antibody Response Against Whole Staphylococcus Aureus In Patients With Staphylococcal Septicaemia and Endocarditis Investigated By ELISA

Jarløv

Christensson

Espersen

et al. 1985

Acta Pathologica Microbiologica Scandinavica Series B: Microbio

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A whole cell Staphylococcus aureus enzyme‐linked immunosorbent assay (ELISA) for the detection of IgG antibodies against S. aureus has been developed. To avoid non‐specific binding of IgG to protein A, the protein A‐poor strains of S. aureus, E 1369 and Wood 46, were used as antigens. Onehundred and eighty serum samples from 120 patients with S. aureus endocarditis, non S. aureus endocarditis, S. aureus septicaemia and non S. aureus septicaemia were tested together with sera from 155 healthy controls. The sensitivity was similar for the E 1369 ELISA and the Wood 46 ELISA and positive test values were detected in 84.2% of patients with S. aureus endocarditis and 41.2% of patients with complicated S. aureus septicaemia. No distinction could be found between complicated and uncomplicated S. aureus septicaemia. The E 1369 ELISA was more specific showing cross reactions with sera from patients infected with other bacteria than S. aureus in 3.6%. Furthermore, the reproducibility was better for the E 1369 ELISA with a coefficient of variation at 0.054. The absence of need for purified antigens makes the whole S. aureus ELISA easy, rapid and cheap. Therefore, we suggest the whole S. aureus ELISA as a good alternative to previously reported assays using purified cell wall antigens.

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“…Because of the difficulties associated with the isolation of the causative agent of osseous infections by routine bacteriological techniques, emphasis has been given recently to the development of serological assays as an aid to diagnosis. The techniques used for the serodiagnosis of serious staphylococcal infections have included gel diffusion (25,34), immunoelectrophoresis (11,25), radioimmunoassay (33,34), and the enzyme-linked immunosorbent assay ELISA (8,10,15,36). The most commonly used staphylococcal antigens for these assays are either ultrasonic preparations of whole cells, ribitol teichoic (TA) acid, or the peptidoglycan (PG) moiety of the cell wall.…”

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confidence: 99%

Antibody response to teichoic acid and peptidoglycan in Staphylococcus aureus osteomyelitis

Jacob¹,

Durham²,

Falk³

et al. 1987

J Clin Microbiol

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An enzyme-linked immunosorbent assay was used to evaluate the immunoglobulin G (IgG) response to Staphylococcus aureus crude teichoic acid (TA) and peptidoglycan (PG) in both rabbits and patients with osteomyelitis. In rabbits with experimental S. aureus osteomyelitis, elevated levels of IgG to TA were present in 13/18 (72%) of the serum samples obtained at 4 and 10 weeks postinfection. In contrast, only 5/18 (28%) of these sera were found to be positive for antibodies to PG. Of a total of 39 patients with confirmed S. aureus osteomyelitis (11 acute, 28 chronic), IgG to TA was elevated in 17 (44%), whereas antibodies to PG were found to be increased in only 1 (3%). Cross-reacting antibodies to S. aureus TA were detected in only 1/18 (6%) of the patients with osteomyelitis caused by organisms other than S. aureus. These studies indicate that IgG to TA is more prevalent than IgG to PG in patients with staphylococcal osteomyelitis. Although these results are encouraging, a larger number of patients is required for an adequate evaluation of the TA enzyme-linked immunosorbent assay for the diagnosis and management of suspected S. aureus osteomyelitis.

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Detection of antibody to Staphylococcus aureus teichoic acid by enzyme-linked immunosorbent assay

Cited by 12 publications

References 9 publications

Comparison of a new enzyme-linked immunosorbent assay method with counterimmunoelectrophoresis for detection of teichoic acid antibodies in sera from patients with Staphylococcus aureus infections

Comparison of a new enzyme-linked immunosorbent assay method with counterimmunoelectrophoresis for detection of teichoic acid antibodies in sera from patients with Staphylococcus aureus infections

Antibody Response Against Whole Staphylococcus Aureus In Patients With Staphylococcal Septicaemia and Endocarditis Investigated By ELISA

Antibody response to teichoic acid and peptidoglycan in Staphylococcus aureus osteomyelitis

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