Detection of Antibody Formation in Mice, Rats and Rabbits Immunized with Different Pneumocystis carinii Antigens

Roth, Andreas; Janitschke, K.

doi:10.1016/s0934-8840(11)80776-0

Cited by 3 publications

(4 citation statements)

References 23 publications

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“…The reactivity of the anti-P. carinii serum with different P. carinii preparations or fungal cells (the latter are outlined below) was studied using an indirect immunofluorescence staining method (IF) as previously described (Roth and Janitschke 1991). In brief, 10 l of the cyst suspension was dried on slides, fixed with methanol, and covered with diluted rabbit anti-P. carinii serum for 15 min at room temperature.…”

Section: Staining Methodsmentioning

confidence: 99%

“…Antibody titers of male New Zealand rabbits against pronasetreated or untreated P. carinii cysts were determined by an indirect immunofluorescence method described elsewhere (Roth and Janitschke 1991). Serum from a rabbit with no detectable antibody against P. carinii was used as a nonimmune control serum.…”

Section: Preparation Of Antibodiesmentioning

confidence: 99%

“…Serum from a rabbit with no detectable antibody against P. carinii was used as a nonimmune control serum. Two rabbits with an antibody titer of 1:10 against pronase-treated cysts from all three hosts were selected for immunization with a soluble antigen preparation obtained by sonication of 10 7 pronase-treated P. carinii cysts extracted from rat lungs as described previously (Roth and Janitschke 1991). The total dose of the antigen was divided into six aliquots of 0.2 ml each.…”

Section: Preparation Of Antibodiesmentioning

confidence: 99%

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Light and electron microscopy study of carbohydrate antigens found in the electron-lucent layer of Pneumocystis carinii cysts

Roth¹,

Wecke

Karsten

et al. 1997

Parasitology Research

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The localization and biochemical nature of antigens found in the electron-lucent layer (ELL) of Pneumocystis carinii cysts using polyclonal rabbit antibodies are described. These antigens, specific for the cystic stages of the parasite, were shared by organisms from different hosts, suggesting that they represent functionally important components of the cyst cell wall. The binding sites were situated on an interwoven net of fibrils in the ELL produced by mild to strong proteolysis. Degradation of this residue by glucanase and chitinase confirms that this layer contains branched glucan and chitin. In contrast, the prompt susceptibility of the polysaccharide-rich ELL to proteolysis reveals that proteins are also relevant in building up the cyst-wall glucan skeleton. It is therefore concluded that the formation of the Pneumocystis cyst wall shows differences to the typical fungal cell-wall architecture. The taxonomical debate regarding this unique protist is ongoing, and consideration of these immunological and morphological findings may be useful for the study of the biology and phylogeny of Pneumocystis.

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Section: Staining Methodsmentioning

confidence: 99%

Section: Preparation Of Antibodiesmentioning

confidence: 99%

Section: Preparation Of Antibodiesmentioning

confidence: 99%

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Light and electron microscopy study of carbohydrate antigens found in the electron-lucent layer of Pneumocystis carinii cysts

Roth¹,

Wecke

Karsten

et al. 1997

Parasitology Research

Self Cite

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“…carinni is an opportunistic fungus that casues a potentially severe and fatal pneumonia (PCP) in a variety of patients with compromised immune status due to AIDS, chemotherapeutic regimes for cancer, immunosuppressive therapy for organ transplant and congenital immune diseases (Wazir and Ansari, 2004). Roth and Janitschke demonstrated the formation of antibodies in mice, rats and rabbits immunized with different P. carinii antigens (Roth and Janitschke, 1991). Hyperimmune sera was highly effective at reducing the number of fungal organisms in early, intermediate, and advanced stages of PCP and was capable of increasing the mean life expectancy of infected mice (Roths and Sidman, 1993) and passive immunization with polyclonal antibodies in sera from animals that were allowed to recover from severe P. carinii pneumonia conferred protection to naïve mice (Bartlett et al, 1998).…”

Section: Pneumocytis Cariniimentioning

confidence: 99%

Antibodies

López-Ribot

Diez-Orejas

Gil

Immunology of Fungal Infections

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Generation of antibodies against fungal antigens is one of the hallmarks of the host/fungus interaction, and antibodies are important contributors to host immunity during these fastidious infections. Many studies have examined the role of antibody production as a prerequisite for protective immunity to different pathogenic fungi, both in vivo and in vitro. Surprisingly, this has proved a problematic issue to definitively determine, mainly because fungal infections have been traditionally associated with defects in cell mediated immunity and because of the heterogeneity of antibodies in polyclonal sera. However, in the recent years there has been a growing body of evidence for the protective role of antibodies and a revival on the interest and potential use of antibodies in novel, immune-based approaches for the management (diagnosis, prevention and treatment) of fungal infections *

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Pneumocystis carinii organisms obtained from rats, ferrets, and mice are antigenically different

et al. 1993

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Pneumocystis carinii infections were developed in animals immunosuppressed by dexamethasone treatment either from activation of latent infection (ferret) or by transtracheal inoculation with P. carinii-infected lung tissue from the homologous species (rat or mouse). Convalescent-phase antisera were obtained by stopping dexamethasone treatment after 2 to 4 weeks and allowing animals 5 to 8 weeks for recovery. P. carinii harvests from infected lungs were purified by differential filtration, solubilized in buffer containing urea, sodium dodecyl sulfate (SDS), and 2-mercaptoethanol, subjected to SDS-polyacrylamide gel electrophoresis, and blotted to polyvinylidene difluoride sheets for Western immunoblot analysis. These lung preparations are hereafter referred to as P. carinii antigens. Convalescent-phase antisera from each animal species were reacted on Western blots of P. carinii antigens prepared from organisms isolated from rats, ferrets, or mice. Each combination of P. carinii antigens and antisera from the same species of animal reacted with three or more P. carinii antigen proteins. Convalescent-phase mouse antisera reacted with P. carinii antigens from mice but not rats or ferrets. Convalescent-phase rat antisera reacted with P. carinii antigens from rats and mice but not ferrets, and convalescent-phase ferret antisera showed reactions with ferret and mouse P. carinii antigens but not rat antigens. These findings indicate antigenic differences among P. carinii strains infecting these animals. Pneumocystis carinii is an opportunistic organism thought to be ubiquitous in the environment. P. carinii infects many animal species besides humans, including rats, ferrets, and mice. Several lines of evidence suggest P. cannii strains infecting each species are different. Intraspecies P. carinii strain variations may also exist (6). Many investigators have studied the antigenic characteristics of P. carinii by using hyperimmune antisera or monoclonal antibodies with various immunologic techniques. Gigliotti et al. (9) determined that P. carinii isolates from different host species are not antigenically identical by using monoclonal antibodies that were reactive to rat P. cannii. Kovacs et al. (11, 12) also used monoclonal antibodies to rat and human P. carinii to demonstrate antigenic differences among P. carinii isolates. Linke et al. (14) analyzed P. cannii antigens for their susceptibilities to treatment with enzymes by immunoblotting and determined that rat and human P. cannii have both shared and species-specific antigenic determinants. Roth and Janitschke (15) used immunofluorescence to demonstrate differences among species with P. carinii by using hyperimmune sera. Convalescent-phase or infection-derived antisera developed in both rats and mice (5, 21, 22) have made it possible to compare naturally occurring antibodies among different species of animals infected with P. carinii (3). Walzer and Linke (19) showed that rat and human P. carinii isolates have both shared and unique antigenic determinants by

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Detection of Antibody Formation in Mice, Rats and Rabbits Immunized with Different Pneumocystis carinii Antigens

Cited by 3 publications

References 23 publications

Light and electron microscopy study of carbohydrate antigens found in the electron-lucent layer of Pneumocystis carinii cysts

Light and electron microscopy study of carbohydrate antigens found in the electron-lucent layer of Pneumocystis carinii cysts

Antibodies

Pneumocystis carinii organisms obtained from rats, ferrets, and mice are antigenically different

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