Detection of antibodies to mycobacterium tuberculosis by solid phase radioimmunoassay

Determination of Antibacterial Antibodies in Serum by Immunoradiometric Assays

“…As found by Nassau et al (1975) and confirmed here, a serious difficulty with the indirect technique is adsorption of non-antibody globulin by antigen or supporting medium, or both, and this subsequently combines with labelled antiglobulin. In our assay system, the response of adsorbed globulin was equivalento 5-10 pg of antibody per ml serum.…”

“…Of the several different principles that can be used as a basis for immunoradiometric assay, two were selected for rigorous assessment. These were, first, competition between antibody in the test sample and added isotopically-labelled homologous antibody for attachment to antigen and, second, attachment of antibody in the test sample to solid-phase antigen, followed by reaction of the resulting complex with isotopically-labelled homologous anti-globulin (Rosenthal, Hayashi and Notkins, 1972;Nielsen, Parratt and White, 1973;Restall, Thomas and Pennant, 1973;Smith, Gehle and McCracken, 1974;Nassau, Parsons and Johnson, 1975). When assay procedures based on the two principles were applied to samples containing known concentrations of immunopurified antibacterial globulins in saline-phosphate buffer, sensitive detection and reproducible results were obtained.…”

Serological diagnosis of tuberculosis: a report of 12 months' clinical experience.

“…Many authors have reported serological tests which have appeared reliable when employed against sera from patients with known diagnoses (Middlebrook and Dubos, 1948;Parlett and Youmans, 1959;Takahashi, 1962;Lind, 1964;Duboczy and White, 1966;Wallace et al, 1966;Cole, Lazarus, and Hedrick, 1972; Affronti, Fife, and Grow, 1973;Nassau, Parsons, and Johnson, 1975;Nicholls, 1975) but none of these has proved of sufficient clinical usefulness to become universally adopted.…”

Analysis of Perchloric-Acid-Soluble <i>Mycobacterium bovi</i><i>s</i> Bacille bilié de Calmette-Guérin Antigen

“…Studies on the serology of tuberculosis began with the applications of the complement fixation test in 1901 [2], and extensive studies were introduced such as hemagglutination test [3j, precip itation and gel diffusion [4], the fluorescent antibodies test [5], radioimmunoassay [6], and enzyme-linked immuno sorbent assay (ELISA) [7]. Dicna [8] summarized the re quirements for the ideal serological test for tuberculosis.…”