Detection of antibodies to <i>Salmonella enteritidis</i> in sera and yolks from experimentally and naturally infected chickens

Desmidt, Maria; Ducatelle, Richard; Haesebrouck, Freddy; Groot, P. A. De; Verlinden, Marc; Wijffels, R.H.; Hinton, M.; Bale, Janet; Allen, V. M.

doi:10.1136/vr.138.10.223

Cited by 47 publications

(19 citation statements)

References 10 publications

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“…At the day of arrival, the Salmonella status of all the animals was tested by bacteriological analysis of cloacal swabs. Sera of 20% of the animals were tested for anti-Salmonella antibodies using a previously described enzyme-linked immunosorbent assay (Desmidt et al, 1996). The animals had free access to drinking water and were fed ad libitum.…”

Section: Methodsmentioning

confidence: 99%

A comparative study on the pathogenesis of egg contamination by different serotypes ofSalmonella

et al. 2008

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Salmonella enterica serotype Enteritidis is the predominant serotype associated with egg-borne salmonellosis in humans. Apparently this serotype possesses particular characteristics that increase its chance to contaminate eggs. To identify these characteristics, two Salmonella serotype Enteritidis strains as well as one strain of each of the serotypes Salmonella Typhimurium, Salmonella Heidelberg, Salmonella Virchow and Salmonella Hadar strain were used to examine different aspects related to egg contamination. After an intravenous infection of laying hens, it was observed that the ability of the serotype Enteritidis strains to colonize the reproductive organs was significantly higher compared with the Salmonella Heidelberg, Salmonella Virchow and Salmonella Hadar strains but not with the Salmonella Typhimurium strain. Inoculating low numbers of the different Salmonella serotypes in egg albumen at 42 degrees C demonstrated that the growth of the strains belonging to the Salmonella serotypes Virchow and Hadar was seriously repressed. The other serotypes, however, survived in albumen for 24 h. Furthermore, using two different specifically designed egg infection models, it was shown that all strains used in this study were able to penetrate into and multiply inside the yolk at 25 degrees C. These findings indicate that the ability to grow in eggs post lay is not specific for the serotype Enteritidis. In conclusion, comparing strains belonging to different Salmonella serotypes has revealed that most probably a preferential colonization of the reproductive organs and an enhanced survival at 42 degrees C allows the serotype Enteritidis to contaminate eggs.

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Section: Methodsmentioning

confidence: 99%

A comparative study on the pathogenesis of egg contamination by different serotypes ofSalmonella

et al. 2008

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“…Antibodies to Salmonella have been detected in the egg albumen and yolk from naturally and experimentally infected chickens (Schiemann & Montgomery, 1991;Desmidt et al, 1996). Antibodies to Salmonella have been detected in the egg albumen and yolk from naturally and experimentally infected chickens (Schiemann & Montgomery, 1991;Desmidt et al, 1996).…”

Section: Antimicrobial Components In the Albumen And Vitelline Membranementioning

confidence: 99%

Mechanisms of egg contamination bySalmonellaEnteritidis

et al. 2009

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Salmonella Enteritidis (SE) has been the major cause of the food-borne salmonellosis pandemic in humans over the last 20 years, during which contaminated hen's eggs were the most important vehicle of the infection. Eggs can be contaminated on the outer shell surface and internally. Internal contamination can be the result of penetration through the eggshell or by direct contamination of egg contents before oviposition, originating from infection of the reproductive organs. Once inside the egg, the bacteria need to cope with antimicrobial factors in the albumen and vitelline membrane before migration to the yolk can occur. It would seem that serotype Enteritidis has intrinsic characteristics that allow an epidemiological association with hen eggs that are still undefined. There are indications that SE survives the attacks with the help of antimicrobial molecules during the formation of the egg in the hen's oviduct and inside the egg. This appears to require a unique combination of genes encoding for improved cell wall protection and repairing cellular and molecular damage, among others.

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“…Eggs were collected in the period January to June 2003 at the middle (34 to 51 weeks of hen age) of lay. No egg out of 30 eggs sampled tested positively for antibiotics (Premi Õ Test-Egg kit) or Salmonella antibodies (Desmidt et al, 1996). Only intact eggs assessed by candling without visual faecal or egg contents contamination of the shell were taken.…”

Section: Eggsmentioning

confidence: 99%

Eggshell penetration of hen's eggs bySalmonella entericaserovar Enteritidis upon various storage conditions

Messens¹,

Grijspeerdt²,

Herman³

2006

British Poultry Science

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1. The survival and penetration of Salmonella enterica serovar Enteritidis (SE) inoculated on the eggshell was examined upon storage for up to 20 d at real-life conditions (15 to 25 degrees C and 45 to 75% relative humidity (RH)). 2. Penetration was assessed by emptying the egg contents and filling the eggs with a selective medium that allowed visualising Salmonella growth on the inside of the shell and membrane complex. 3. The study of survival on the eggshells was based on viable counts and showed that numbers of surviving organisms decreased over time. Survival was inversely related to storage temperature and RH. Although the average counts decreased over time, a limited proportion of shells carried high numbers of SE at all storage conditions. 4. Penetration spots were observed earlier using an increased storage temperature due to increased growth rates of SE on the agar. After 20 d of storage a similar percentage (c. 44.7%) of eggshells became penetrated, irrespective of the storage conditions tested in this study. 5. The higher the Salmonella shell contamination at the end of storage, the higher the probability that the eggshell was penetrated. Salmonella shell counts exceeding 4 log cfu yielded more than a 90% probability of eggshell penetration occurring.

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Detection of antibodies to Salmonella enteritidis in sera and yolks from experimentally and naturally infected chickens

Cited by 47 publications

References 10 publications

A comparative study on the pathogenesis of egg contamination by different serotypes ofSalmonella

A comparative study on the pathogenesis of egg contamination by different serotypes ofSalmonella

Mechanisms of egg contamination bySalmonellaEnteritidis

Eggshell penetration of hen's eggs bySalmonella entericaserovar Enteritidis upon various storage conditions

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