Detection of Antibodies to Herpes Simplex Virus in the Cerebrospinal Fluid of Patients with Herpes Simplex Encephalitis

Kahlon, Jasbir; Chatterjee, Sabyasachi; Lakeman, Fred D.; Lee, Frances; Nahmias, André J.; Whitley, Richard J.

doi:10.1093/infdis/155.1.38

Cited by 64 publications

(23 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Because speci®c therapy is available for HSV [23] and certain other agents, precise aetiological diagnosis is important [9,11,20]. Numerous diagnostic tests have been developed to assist in this precision [3,6,13]. We performed a multicentre study, which covered 85% of the child population in Finland, and prospectively collected clinical data and microbiological samples from patients with encephalitis.…”

Section: Introductionmentioning

confidence: 99%

Epidemiology of encephalitis in children. A prospective multicentre study

Koskiniemi¹,

Korppi

Mustonen

et al. 1997

European Journal of Pediatrics

121

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Epidemiology of encephalitis in children. A prospective multicentre study

Koskiniemi¹,

Korppi

Mustonen

et al. 1997

European Journal of Pediatrics

121

View full text Add to dashboard Cite

show abstract

“…There is only one reported study available which determines immunity induced by peptides corresponding to the antigenic sites of gB (Mester et al, 1990). It is reported that antibodies in CSF against gB in HSV were detected earlier than antibodies to the other viral proteins, probably because of high immunogenicity of gB (Kahlon et al, 1987). One study demonstrated that gB is presented with the same kinetics as a classical early-gene product and it is the possibility that gB could be an effective target as HSV emerges from latent infection (Mueller et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Identification of an immunodominant epitope in glycoproteins B and G of herpes simplex viruses (HSVs) using synthetic peptides as antigens in assay of antibodies to HSV in herpes simplex encephalitis patients

Ss¹,

Nh²,

Nn³

et al. 2014

View full text Add to dashboard Cite

Summary. -Herpes simplex encephalitis (HSE) is a severe viral infection of the central nervous system (CNS). Assay of antibody response is widely used in diagnostics of HSE. The aim of this study was to identify an immunodominant epitope determining the antibody response to herpes simplex viruses (HSVs) in cerebrospinal fluid (CSF) of HSE patients. The synthetic peptides that resembled type-common as well as type-specific domains of glycoproteins B (gB) and G (gG) of these viruses were evaluated for binding with IgM and IgG antibodies in CSF samples from HSE and non-HSE patients in ELISA. The QLHDLRF peptide, derived from gB of HSV was found to be an immunodominant epitope in the IgM and IgG antibody response. The patients with confirmed and suspected HSE showed in ELISA against this peptide 26% and 23% positivities for IgM, 43% and 37% positivities for IgG and 17% and 15% for both IgM and IgG antibodies, respectively. The total positivities of 86% and 75% for both IgM and IgG antibodies were obtained in the patients with confirmed and suspected HSE, respectively. These results demonstrate that a synthetic peptide-based diagnostics of HSE can be an efficient and easily accessible alternative. This is the first report describing the use of synthetic peptides derived from HSVs in diagnostics of HSE using patientsʹ CSF samples.

show abstract

“…A RM, embora muito superior aos demais métodos neurorradiológicos, pode apresentar alterações semelhantes às vistas em outras patologias [14][15][16] . Os métodos de detecção de anticorpos no LCR apresentam pequena sensibilidade na fase aguda da doença e baixa reprodutibilidade [17][18][19][20][21][22] . O isolamento viral a partir de tecido cerebral obtido por biópsia é método sensível, específico e reprodutível, mas altamente agressivo [23][24][25][26] .…”

Section: Discussão Discussão Discussão Discussão Discussãounclassified

Diagnósticos alternativos em pacientes com suspeita de encefalite por Herpes simplex e negativos à reação em cadeia por polimerase (PCR)

Domingues

Pannuti

Fink

et al. 2000

Arq. Neuro-Psiquiatr.

View full text Add to dashboard Cite

RESUMO -O objetivo do presente estudo é analisar os diagnósticos encontrados em uma série de pacientes cuja suspeita clínica inicial era de encefalite herpética (HSE), mas que tiveram este diagnóstico afastado através de resultado negativo à reação em cadeia por polimerase (PCR) para detecção do Herpes simples (HSV) em líquido cefalorraqueano (LCR). Em 43 dos 61 pacientes com suspeita de HSE estudados (70,5%) o resultado à PCR foi negativo. O diagnóstico diferencial foi elucidado em 41,9% dos 43 casos em que a PCR para HSV resultou negativa. Nestes, as patologias diagnosticadas foram infecções virais (2 casos-11,1%) e não virais (5 casos-27,2%), doenças vasculares (4 casos-22,2%), desmielinizantes (3 casos-16,7%), distúrbios tóxico-metabólicos (3 casos-16,7%) e tumor do sistema nervoso central (1 caso-5,6%). A pouca especificidade do quadro clínico e a disponibilidade de tratamento eficaz e seguro para a HSE justificam a grande quantidade de casos tratados com aciclovir, mas cujo diagnóstico de encefalite pelo HSV não foi confirmado. A utilização da PCR no LCR contribuiu para melhor avaliação etiológica dos quadros de encefalite aguda aqui estudados. PALAVRAS-CHAVE: encefalite herpética, reação em cadeia por polimerase (PCR), diagnóstico diferencial. Alternative diagnoses among suspected Herpes simplex encephalitis patients with negative polymerase chain reaction (PCR)ABSTRACT -The aim of this study was to analyze the diagnosis found in a series of patients in which the diagnosis of Herpes simplex encephalitis (HSE) was ruled out by a negative polymerase chain reaction (PCR) result for HSV DNA in cerebrospinal fluid (CSF) samples. Forty three out of 61 HSE suspected patients had negative PCR. An alternative diagnosis was established in 41.9% of these patients. These patients were diagnosed as having viral (2 cases-11.1%) and non viral (5 cases-27.2%) CNS infections, vascular (4 cases-22.2%) and demyelinating diseases (3 cases-16.7%), metabolic disturbances (3 cases-16.7%), and CNS tumor (1 case-5.6%). The non specific clinical presentation of this disease and the availability of an efficient treatment for HSE explain why several patients with other diseases were initially treated with acyclovir. The early use of PCR in CSF was considered essential for the evaluation of the acute encephalitis cases in this study.KEY WORDS: Herpes simplex encephalitis, polymerase chain reaction (PCR), differential diagnosis.A encefalite pelo vírus Herpes simples (HSV) é doença grave, com altos índices de morbidade e letalidade 1 . O tratamento antiviral com o aciclovir melhora significativamente o prognóstico desta doença, particularmente quando ele é administrado precocemente 2 . Assim, o diagnóstico precoce desta patologia é fundamental. Entretanto, diversas patologias podem ser confundidas com a encefalite

show abstract

Detection of Antibodies to Herpes Simplex Virus in the Cerebrospinal Fluid of Patients with Herpes Simplex Encephalitis

Cited by 64 publications

References 24 publications

Epidemiology of encephalitis in children. A prospective multicentre study

Epidemiology of encephalitis in children. A prospective multicentre study

Identification of an immunodominant epitope in glycoproteins B and G of herpes simplex viruses (HSVs) using synthetic peptides as antigens in assay of antibodies to HSV in herpes simplex encephalitis patients

Diagnósticos alternativos em pacientes com suspeita de encefalite por Herpes simplex e negativos à reação em cadeia por polimerase (PCR)

Contact Info

Product

Resources

About