Detection of antibodies to Eperythrozoon ovis by the use of an enzyme-linked immunosorbent assay

Lang, F.; Ferrier, GR; Nicholls, T. J.

doi:10.1016/s0034-5288(18)30782-3

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…Daddow ( 1977 ) developed a complement fixation test (CFT) using antigens prepared from lysed red blood cells for serological detection of E. ovis in sheep, but the application of CFT was limited to the detection of only new infections. Lang et al ( 1987 ) developed the enzyme-linked immunosorbent assay (ELISA) for the detection of serum antibody to E ovis in sheep and is still in current use as a confirmatory test for diagnosis of M. ovis infection in small ruminants (Alleman et al 1999 ; Abed and Alsaad 2017 ). Compared to CFT and IFAT, the ELISA is the preferred test for serodiagnosis and epidemiological survey of small ruminant flocks (Kanabathy and Nachiar 2004 ).…”

Section: Diagnosis Of Mycoplasma Ovismentioning

confidence: 99%

Review of clinical aspects, epidemiology and diagnosis of haemotropic Mycoplasma ovis in small ruminants: current status and future perspectives in tropics focusing on Malaysia

Paul

Jesse

Chung

et al. 2020

Trop Anim Health Prod

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Mycoplasma ovis (formerly Eperythrozoon ovis ) is an epierythrocytic parasitic bacterium of small ruminants known as haemotropic mycoplasma, which is transmitted mechanically by biting flies and contaminated instruments. Acute mycoplasmosis causes severe haemolytic anaemia and mortality in young animals. At the same time, chronic disease may produce mild anaemia and varying degrees of morbidity depending on several factors, including age, reproductive status, the plane of nutrition, immunological status and the presence of concurrent infection. Haemotropic Mycoplasma ovis is currently recognised as an emerging zoonotic pathogen which is widely distributed in the sheep and goat producing areas of tropics and subtropics, where the disease is nearly endemic. Human infection has been reported in pregnant women, immunocompromised patients and people exposed to animals and arthropods. The current diagnosis of haemoplasma relies on microscopic evaluation of Giemsa-stained blood smear and PCR. Although there are few published reports on the incidence of haemotropic Mycoplasma ovis infection of small ruminants in Malaysia, information on its prevalence, risk factors, severity and economic impacts is grossly inadequate. Therefore, a large-scale survey of small ruminant flocks is necessary to elucidate the current seroprevalence status and molecular characteristics of haemotropic M. ovis infection in Malaysia using ELISA and PCR sequencing technologies. In the future, surveillance programs, including vector forecast, quarantine, monitoring by periodic surveys and public enlightenment, will limit the internal and transboundary spread of M. ovis , enhance control efforts and mitigate production losses in Malaysia. Electronic supplementary material The online version of this article (10.1007/s11250-020-02357-9) contains supplementary material, which is available to authorized users.

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Section: Diagnosis Of Mycoplasma Ovismentioning

confidence: 99%

Review of clinical aspects, epidemiology and diagnosis of haemotropic Mycoplasma ovis in small ruminants: current status and future perspectives in tropics focusing on Malaysia

Paul

Jesse

Chung

et al. 2020

Trop Anim Health Prod

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“…Serum was also analysed for Ep. ovis infection by an enzyme-linked immunosorbent assay (ELISA) test [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

Persistence of Granulocytic EhrlichiaInfection During Wintertime in Two Sheep Flocks in Norway

Stuen¹,

Djuve

Bergström

2001

Acta Vet Scand

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Ixodes ricinus. In this study, 2 sheep flocks that had been grazing on I. ricinus infested pastures the previous season, were blood sampled after being housed indoors for nearly 6 months during wintertime. Thirty animals from each flock were examined for granulocytic Ehrlichia infection in the peripheral blood by blood inoculation studies, stained blood smear evaluation, polymerase chain reaction (PCR) analysis and serology (IFAantibodies). The animals were sampled twice within a three-week period, the first time before and the second time after lambing. Two sheep in one flock were found Ehrlichia positive by both blood smear evaluation and PCR before lambing, and 3 sheep were found positive after lambing; 2 by blood smear examination and 3 by PCR. In the other flock, no sheep was found infected before lambing, but 2 ewes were found positive after lambing by both blood smear evaluation and PCR. In the first flock, 87% of the animals were found seropositive before lambing, and the mean antibody titre (log 10 ± SD) to E. equi was 2.45 ± 0.401. In the second flock, 40% were found seropositive before lambing, and the mean antibody titre was 1.93 ± 0.260. Seroprevalence and mean antibody titre in these 2 flocks were significantly different (p<0.001). The present study indicates that sheep may be a reservoir host for granulocytic Ehrlichia infection from one grazing season to the next under natural conditions in Norway.antibodies; PCR; blood smear; reservoir host; Ehrlichia phagocytophila; tick-borne fever.

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“…At present, direct microscopy is almost the most common method used in clinical checkups. Very few domestic reports associated with serology diagnosis have been published, whereas there are many methods which have been reported abroad, such as CFT (Daddow, 1977;Splitter, 1985), IHA (Smith and Rahn, 1975;Baljer et al, 1989), ELISA (Nicholls and Veal, 1986;Lang et al, 1987;Schuller et al, 1990;Hsu et al, 1992;Cha and Liu, 2002;Zhang and Zhang, 2006), IFA (Hua et al, 1970) etc. However, a lot of factors could cause morphological changes in erythrocytes, and antibodies often present transient features so the methods above usually result in misdiagnosis (Mason and Statham, 1991;Huang et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Detection of Eperythrozoon wenyoni by PCR assay

Wang

Zhu

Qin

et al. 2009

Front. Agric. China

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The objective of this research was to develop a detection method for Eperythrozoon wenyoni infection using polymerase chain reaction (PCR) assay technique. A pair of primers was designed and synthesized according to the conservative sequence 16S rRNA. The PCR assay was performed with the primers. A 985-bp fragment was amplified by using PCR. The amplified fragments with the expected size were identified by EcoR I restriction digestion. The crossing-reaction, specific-reaction and duplicate-reaction indicated that the PCR method is a specific, sensitive, fast and effective method for diagnosing E. wenyoni infection at group level.

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Detection of antibodies to Eperythrozoon ovis by the use of an enzyme-linked immunosorbent assay

Cited by 4 publications

References 10 publications

Review of clinical aspects, epidemiology and diagnosis of haemotropic Mycoplasma ovis in small ruminants: current status and future perspectives in tropics focusing on Malaysia

Review of clinical aspects, epidemiology and diagnosis of haemotropic Mycoplasma ovis in small ruminants: current status and future perspectives in tropics focusing on Malaysia

Persistence of Granulocytic EhrlichiaInfection During Wintertime in Two Sheep Flocks in Norway

Detection of Eperythrozoon wenyoni by PCR assay

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