Detection of Antibodies to Alphaviruses and Discrimination between Antibodies to Eastern and Western Equine Encephalitis Viruses in Rabbit Sera using a Recombinant Antigen and Virus‐specific Monoclonal Antibodies

Pässler, S.; Pfeffer, Martin

doi:10.1046/j.1439-0450.2003.00669.x

Cited by 13 publications

(4 citation statements)

References 25 publications

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“…Because of a statistically important difference between the results of the two methods, it is thought that ELISA is advantageous for serological diagnosis of CCoV infection. Such results had also been found for different agents (Paessler and Pfeffer, 2003).…”

Section: Discussionsupporting

confidence: 77%

Canine Coronavirus Infection in Turkish Dog Population

Yeşilbağ¹,

Yılmaz

Torun

et al. 2004

Journal of Veterinary Medicine, Series B

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Canine coronavirus (CCoV) is one of the most important viral agents affecting the gastrointestinal system of dogs. In this study virological and serological investigations were performed to demonstrate the existence and prevalence of CCoV infection in a Turkish dog population. A total of 269 animals were subjected to the study. Of 179 dogs tested for CCoV antibodies, 112 (62.5%) were found to be positive by serum neutralization test, while 133 (74.3%) were positive by ELISA. The highest prevalence (94.2%) was detected in kennel dogs. Detection of CCoV genome in faeces was performed in samples from 90 diarrhoeic puppies by reverse transcription-polymerase chain reaction. Fourteen (15.5%) faeces were positive for CCoV RNA, five of which were characterized as CCoV type I. The widespread CCoV infection in the Turkish dog population may be attributed as an important cause of viral diarrhoea in dogs.

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Section: Discussionsupporting

confidence: 77%

Canine Coronavirus Infection in Turkish Dog Population

Yeşilbağ¹,

Yılmaz

Torun

et al. 2004

Journal of Veterinary Medicine, Series B

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“…For epidemiological studies in animal populations, competitive assays or methods using protein A‐peroxidase conjugates offer the advantage to be applicable for different animal species (Guzaeva et al., 1993; Pässler and Pfeffer, 2003).…”

Section: Serological Assays For the Diagnosis Of Tularaemiamentioning

confidence: 99%

Diagnostic Procedures in Tularaemia with Special Focus on Molecular and Immunological Techniques

Splettstoesser

Tomaso

Dahouk

et al. 2005

Journal of Veterinary Medicine, Series B

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Tularaemia is a severe bacterial zoonosis caused by the highly infectious agent Francisella tularensis. It is endemic in countries of the northern hemisphere ranging from North America to Europe, Asia and Japan. Very recently, Francisella-like strains causing disease in humans were described from tropical northern Australia. In the last decade, efforts have been made to develop sensitive and specific immunological and molecular techniques for the laboratory diagnosis of tularaemia and also for the definite identification of members of the species F. tularensis and its four subspecies. Screening for the keyword 'Francisella' a Medline search over the last decade was performed and articles describing diagnostic methods for tularaemia and its causative agent were selected. Besides classical microbiological techniques (cultivation, biochemical profiling, susceptibility testing) several new immunological and molecular approaches to identify F. tularensis have been introduced employing highly specific antibodies and various polymerase chain reaction (PCR)-based methods. Whereas direct antigen detection by enzyme-linked immunosorbent assay (ELISA) or immunofluorescence might allow early presumptive diagnosis of tularaemia, these methods--like all PCR techniques--still await further evaluation. Therefore, diagnosis of tularaemia still relies mainly on the demonstration of specific antibodies in the host. ELISA and immunoblot methods started to replace the standard tube or micro-agglutination assays. However, the diagnostic value of antibody detection in the very early clinical phase of tularaemia is limited. Francisella tularensis is regarded as a 'highest priority' biological agent (category 'A' according to the CDC, Atlanta, GA, USA), thus rapid and reliable diagnosis of tularaemia is required not only for a timely onset of therapy, the handling of outbreak investigations but also for the surveillance of endemic foci. Only very recently, evaluated test kits for serological diagnosis of human tularaemia became available, while the introduction of standardized molecular techniques for detection and typing is still missing.

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“…However, due to the similarity of the neutralization domains of closely related variety ID/IE and IAB/C VEEV strains, the degree of cross-neutralization is very high making the final diagnosis using the PRNT difficult. To overcome these problems, we developed an epitopeblocking-ELISA, similar to previously published assays for detection of antibodies to flaviviruses [16][17][18] and alphavirus, 19 which is able to distinguish between infections with enzootic, variety ID/E/F and epizootic, variety IAB/C VEEV strains.…”

Section: Introductionmentioning

confidence: 99%

A Novel, Rapid Assay for Detection and Differentiation of Serotype-Specific Antibodies to Venezuelan Equine Encephalitis Complex Alphaviruses

Wang

Paessler

Aguilar

et al. 2005

The American Journal of Tropical Medicine and Hygiene

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An epitope-blocking enzyme-linked immunosorbent assay was developed for the rapid differentiation of serologic responses to enzootic variety IE and ID versus epizootic variety IAB and IC strains of Venezuelan equine encephalitis (VEE) virus. Two monoclonal antibodies that differentially recognize epizootic versus enzootic VEE virus epitopes were used to measure the serotype-specific blocking abilities of antibodies in sera of naturally infected humans, equines, and bovines, as well as in experimentally infected equines. The assay is simple, species-independent, rapid, and sensitive, and will improve surveillance for VEE emergence. It could also be used to determine the epidemic potential of a VEE virus following an intentional introduction for bioterrorism.

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Detection of Antibodies to Alphaviruses and Discrimination between Antibodies to Eastern and Western Equine Encephalitis Viruses in Rabbit Sera using a Recombinant Antigen and Virus‐specific Monoclonal Antibodies

Cited by 13 publications

References 25 publications

Canine Coronavirus Infection in Turkish Dog Population

Canine Coronavirus Infection in Turkish Dog Population

Diagnostic Procedures in Tularaemia with Special Focus on Molecular and Immunological Techniques

A Novel, Rapid Assay for Detection and Differentiation of Serotype-Specific Antibodies to Venezuelan Equine Encephalitis Complex Alphaviruses

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