Detection of antibodies specific for sheeppox and goatpox viruses using recombinant capripoxvirus antigens in an indirect enzyme-linked immunosorbent assay

Bowden, Timothy R.; Coupar, Barbara E.H.; Babiuk, Shawn; White, John R.; Boyd, Victoria; Duch, Christine J.; Shiell, Brian J.; Ueda, Norihito; Parkyn, Geoff; Copps, John; Boyle, David B.

doi:10.1016/j.jviromet.2009.04.031

Cited by 55 publications

(46 citation statements)

References 36 publications

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“…[17] Reactivities of the antisera with capripoxvirus proteins, including protein 95, have been characterized previously. [17] RESULTS AND DISCUSSION…”

Section: Assessment Of Purified Capripoxvirus Protein Reactivity By Ementioning

confidence: 99%

“…[17] Each construct was used to transform chemically competent BL21-AI E. coli. Individual colonies were subcultured and glycerol stocks were prepared by dilution 1:2 with 80% glycerol then stored at À80 C.…”

Section: Preparation Of Plasmids and Bacterial Strainmentioning

confidence: 99%

“…This protein band has been observed previously in LB medium and was identified by mass spectroscopy as a C-terminally truncated form of protein 95. [17] Although fortuitous, how EnBase media affected formation of this protein product is unknown, but it was not related to the addition of EnBase booster.…”

Section: Total Recombinant Protein Recovery Increased In Cultures Gromentioning

confidence: 99%

“…The chosen proteins have been described previously. [17] Selection was based on known significant differences in the growth rate of each culture and the solubility of each protein.…”

Section: Introductionmentioning

confidence: 99%

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Increased Bacterial Cell Density and Recombinant Protein Yield Using a Commercial Microbial Cultivation System

Peck

Bowden

Shiell

et al. 2013

Preparative Biochemistry and Biotechnology

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EnBase (BioSilta, Finland) is a microbial cultivation system that replicates fed-batch systems through sustained release of glucose by enzymatic degradation of a polymeric substrate. Achievable bacterial cell densities and recombinant capripoxvirus protein expression levels, solubility, and antigenicity using the EnBase system were assessed. BL21-AI Escherichia coli expressing capripoxvirus proteins achieved up to eightfold higher cell densities when grown in EnBase media compared with standard media. Greater yields of capripoxvirus proteins were attained using EnBase media, either through increases in the amount of expressed protein per cell in conjunction with higher cell density or through the increase in cell density alone. Addition of EnBase booster enhanced protein yield for one of the proteins tested but reduced yield for the other. However, the amount of soluble forms of the capripoxvirus proteins tested was not different from that observed from cultures grown under standard conditions. Purified capripoxvirus proteins expressed using EnBase or standard media were assessed for their performance by enzyme-linked immunosorbent assay (ELISA) and were shown to be equally capable of specifically binding capripoxvirus antibodies.

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“…[17] Reactivities of the antisera with capripoxvirus proteins, including protein 95, have been characterized previously. [17] RESULTS AND DISCUSSION…”

Section: Assessment Of Purified Capripoxvirus Protein Reactivity By Ementioning

confidence: 99%

Section: Preparation Of Plasmids and Bacterial Strainmentioning

confidence: 99%

Section: Total Recombinant Protein Recovery Increased In Cultures Gromentioning

confidence: 99%

“…The chosen proteins have been described previously. [17] Selection was based on known significant differences in the growth rate of each culture and the solubility of each protein.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Increased Bacterial Cell Density and Recombinant Protein Yield Using a Commercial Microbial Cultivation System

Peck

Bowden

Shiell

et al. 2013

Preparative Biochemistry and Biotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Geographic regions affected by sheep pox and goat pox have been mainly limited to Africa and middle Asia in the last 50 years [4][5][6][7], but extended to Bangladesh in 1984 [8] and India [9,10], and more recently to Yemen, Vietnam [11] and Mongolia [12]. Repeated incursions have been reported in Greece and in southern Europe [1].…”

Section: Introductionmentioning

confidence: 99%