Detection of antibodies against Leishmania infantum in cats (Felis catus) from the State of Pernambuco, Brazil

Silva, Rita de Cássia Nascimento; Ramos, Rafael Antonio Nascimento; Pimentel, Danillo de Souza; Oliveira, Gênova Maria de Azevedo; Carvalho, Gílcia Aparecida de; Santana, Marília de Andrade; Faustino, Maria Aparecida da Glória; Alves, Lêucio Câmara

doi:10.1590/0037-8682-0005-2012

Cited by 21 publications

(17 citation statements)

References 11 publications

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“…The presence of anti-Leishmania spp. antibodies in cats has also been reported in other regions in Brazil, such as Barra Mansa, state of Rio de Janeiro (Figueiredo et al 2009) and Recife and Petrolina, state of Pernambuco (Silva et al 2014).…”

Section: Introductionmentioning

confidence: 60%

Leishmania infantum in domestic cats from the municipality of Teresina, state of Piauí, Brazil

et al. 2017

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S U M M A R YFeline leishmaniasis has been reported in various parts of the world in recent years, occurring mainly in areas where zoonotic visceral leishmaniasis (VL) is endemic. The purpose of this study was to assess the occurrence of natural infection by Leishmania spp. in domestic cats (Felis catus) in the municipality of Teresina, Piauí state, Brazil, an endemic area of VL in Brazil. The prevalence of infection by Leishmania spp. in the population under study was 4% (3/83) in the enzyme-linked immunosorbent assay (ELISA) and 4% (3/83) by smear observation and isolation in a culture medium, using popliteal lymphnode sample. Only one of the three infected cats was positive for ELISA, also being positive for feline immunodeficiency virus. In the haematologic parameters, normocytic normochromic anaemia was the most common change, being present in the three infected animals. In the biochemical measurements also were observed alterations in infected animals. The Leishmania spp. strains isolated from the culture medium were characterized as L. infantum. The presence of L. infantum infection in cats in the city of Teresina, an area endemic for VL, suggests the possible involvement of these animals in the epidemiological chain of L. infantum in high-transmission areas.

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Section: Introductionmentioning

confidence: 60%

Leishmania infantum in domestic cats from the municipality of Teresina, state of Piauí, Brazil

et al. 2017

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“…Most likely, the modifi cation of the environment by humans and the sanitary problems, still present in many areas of the country, have contributed to this current picture. Recently, a serological survey was performed in cats from the same area, with prevalence reaching 3.9% (12) . The putative role of these animals in the epidemiology of the disease has been speculated, but more data are needed to better clarify this hypothesis.…”

Section: Conflict Of Interestmentioning

confidence: 99%

Prevalence of zoonotic visceral leishmaniasis in dogs in an endemic area of Brazil

Pimentel¹,

Ramos

Santana

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Introduction:The northeast region of Brazil is endemic for zoonotic visceral leishmaniasis (ZVL). The aim of this study was to determine the prevalence of infection in dogs in Petrolina. Methods: Blood samples were collected from dogs (n = 600), and bone-marrow biopsy was performed in animals with positive serological test results that presented clinical signs of ZVL. The serological analyses were performed using an enzyme-linked immunosorbent assay (ELISA) (S7 ® Biogene). Results: Of the 600 dogs tested, 19% (115/600) presented anti-L. infantum chagasi antibodies. Conclusions: Our data are important because canine infection is an important risk factor for the human disease.

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“…Prevalence of the disease in the study area was high when compared to some works [4,41], who obtained a rate of 3.9% in a cohort of 153 cats using ELISA in the search for infection with L. Infantum, and rates of 2.8% and 1.9 % in a sample of 316 cats using ELISA and direct agglutination. The same occurred in others works [1,33], with 4.29% in a group of 233 animals, and with 0.9% in a sample of 110 felines, both ones carried out using IFI in studies of visceral leishmaniasis.…”

Section: Discussionmentioning

confidence: 49%

“…With respect to the clinical aspect, the presence of four animals classically symptomatic for ATL, among those with detectable immune response, oppose the high rate of asymptomatic feline leishmaniasis previous described for the visceral form [22]. Nevertheless, such result approximates that other [41], who obtained two symptomatic felines from four serum reagent cats in search for L. infantum. As for the non-symptomatic serum reagent animals, they evince the contact with the parasite without evolution of the clinical disease process.…”

Section: Discussionmentioning

confidence: 67%

Serological Diagnosis of Feline Tegumentary Leishmaniasis by Indirect Immunofluorescence (IFI) and Enzyme-Linked Immunosorbent Assay (ELISA) in an Endemic Area in Brazil

Figueiredo

Nascimento

Vasconcelos

et al. 2016

Acta Scientiae. Vet.

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Background: American tegumentary leishmaniasis (ATL) is a serious public health problem, and the participation of domestic cats in its epidemiological process has not yet been fully elucidated. Therefore, the standardization of monitoring methodologies of cat populations becomes important for the generation of information on the disease. In Brazil, ATL presents a wide geographical distribution, being Leishmania (Viannia) braziliensis its etiologic agent of ATL in Rio de Janeiro. The main objective of the present study was investigate the presence of specific anti-Leishmania (Viannia) braziliensis Immunoglobulin G (IgG) in 34 cats from an ATL endemic area in this municipality.Materials, Methods & Results: Sera from three cats from the study area naturally infected with Leishmania (Viannia) braziliensis were used as positive control. Analyses were performed with antigen preparations using indirect immunofluorescence (IFI) and enzyme-linked immunosorbent assay (ELISA). We found prevalence rates of 20.6% (7/34) in the IFI and 14.7% (5/34) in the ELISA. Specificity was 87.1% for the IFI and 93.5% for the ELISA, and both tests presented sensitivity of 100%. Concordance between the used tests was assessed as moderate.Discussion: By conducting a feline serological survey in an endemic area for ATL we provide information on the involvement of this species in such epidemiological process. In this context, the participation of the cat in cycles of leishmaniases has been highlighted by several authors; infection of the phlebotomine by parasites originated from this vertebrate host can already be demonstrated by xenodiagnosis and several cases of feline infection have been reported in endemic regions of several countries such as Brazil, Spain, France, Portugal, Greece, Iran, Israel and Italy. Therefore, the standardization of diagnostic techniques that support the screening and monitoring of such populations becomes of utmost importance. In fact, the use of the IFI and ELISA serological techniques, showing sensitivity and NPV of 100%, confirm the use of such tools in epidemiological surveys for the detection of anti-L. (V.) braziliensis antibodies in domestic cats, and these methodologies are frequently employed in canine surveys showing good results. The serological titers found in this study for Leishmania (V.) braziliensis are similar to those of studies carried out with cats in endemic areas of visceral leishmaniasis in Spain. This similarity shows that the titers can be high even when L. (V.). braziliensis is the etiological agent. It is worth mentioning that these values are significant when compared with those of canine studies carried out in ATL endemic regions. Ultimately, the sample refers to an ATL endemic region located in Brazil, one of the countries with the highest endemicity levels of cutaneous leishmaniasis in the world and, therefore, an important area for the implementation and evaluation of diagnostic techniques for the generation of data on the disease and its possible hosts. Thus, the present study provides information on the occurrence of ATL by Leishmania (V.) braziliensis in domestic cats; demonstrates that the methodologies employed were favorable to the use of such techniques as tools in epidemiological surveys for the diagnosis of feline tegumentary leishmaniasis; and paves the way for further studies on the subject.

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Detection of antibodies against Leishmania infantum in cats (Felis catus) from the State of Pernambuco, Brazil

Cited by 21 publications

References 11 publications

Leishmania infantum in domestic cats from the municipality of Teresina, state of Piauí, Brazil

Leishmania infantum in domestic cats from the municipality of Teresina, state of Piauí, Brazil

Prevalence of zoonotic visceral leishmaniasis in dogs in an endemic area of Brazil

Serological Diagnosis of Feline Tegumentary Leishmaniasis by Indirect Immunofluorescence (IFI) and Enzyme-Linked Immunosorbent Assay (ELISA) in an Endemic Area in Brazil

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