Detection of Anti-Livin Antibody in Gastrointestinal Cancer Patients

Abstract

mL, and mean arterial pressure was significantly correlated with PHF concentrations [ Fig. 1C; slope, 0.008014 Ϯ 0.003734; y-intercept, Ϫ0.6246 Ϯ 0.3908 units/mL; R ϭ 0.394, P ϭ 0.0417 (significant); n ϭ 27].Our goal was to develop MAbs with high affinities to both the rat and human PHF antigens. The antibodies produced were selective for PHF, as demonstrated in the bioassay and cross-reactivity studies. Results generated with the PHF ELISA to detect PHF in biological samples correlated well with results obtained for PHF with the bioassay. The ELISA offers a distinct advantage over the bioassay in that it is faster, less expensive, and quantitative, making it a more reliable system. Although the antigen used in this ELISA was not highly purified and the structure has not been completely elucidated, the strong correlation between this ELISA and the bioassay indicate that this assay may be useful in studies that will further our understanding of this substance. Highly pure antigen, as well as improved understanding of the etiology of PHF-related disease, will be required for this assay to be used as a routine clinical tool.In the normotensive group, PHF was not correlated with blood pressure, but in the hypertensive group PHF was correlated with blood pressure. The number of hypertensive samples was much lower than the number of normotensive samples because of the restriction placed on sample selection. In most cases, once people are diagnosed with high blood pressure, therapy is initiated. Blood pressures in the hypertensive group were therefore in the lower range of hypertension. In both groups, there were some values that fell outside the expected correlation. This observation is not unexpected because our previous studies have demonstrated that PHF may occur in some patients with other conditions, such as type 2 diabetes mellitus (14 ). PHF expression appears to precede increases in systemic blood pressure, and because hypertension is a heterogeneous disease, not all hypertensive patients express PHF.Creation of an ELISA for PHF in biological samples may facilitate future research into the biology and pathology of PHF in humans. Future clinical studies with larger sample sizes will be required to clearly define the relationship of PHF to hypertension. Availability of a test with which clinicians may subclassify hypertension may ultimately allow for selection of the most appropriate therapeutic regimen without requiring the empirical approach that is currently used.We would like to acknowledge the technical contributions of Lynda Tang and Lei Zhang. This work was supported by University-Industry Grant UI-13521 from the Medical Research Council of Canada (to C.G.B.).

Challenge and promise: roles for Livin in progression and therapy of cancer

“…In Livin-positive models, antibodies could interfere with the antiapoptoic effects and are expected to increase apoptosis rate. Actually, it was Watanabe et al who detected anti-Livin antibody in gastrointestinal cancer patients (69). In their study, a huge range of cancer patients of different origins, e.g., breast, renal, melanoma, and lung tumors, were positive for anti-Livin antibodies, suggesting that antiLivin antibodies could be useful for detecting these cancers and can serve as a therapeutic approach (70 -72).…”

Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients

“…Moreover, the protein level was statistically significantly lower in the CRC patients than in the healthy controls. Yagihashi et al (30) assessed serum survivin in various cancers of the digestive system. Survivin antibodies were noted in 39.7% of all cancers studied, including 33% cases of CRC, hepatoma and esophageal cancer, 40% of gastric cancer cases, 42% of pancreatic ductal adenocarcinoma cases and 43% of biliary-tract cancer cases.…”

RNAi-mediated knockdown of target genes: a promising strategy for pancreatic cancer research

“…Increasing evidence indicates that X-linked inhibitor of apoptosis (XIAP), Survivin and Livin, members of inhibitors of the apoptosis protein (IAP) family, are confirmed to be upregulated in many human type tumors, which, conversely, are partly crucial to the chemoradioresistance. 51,52 Survivin overexpression correlated with poor prognoses in pancreatic cancer patients, as well as in other malignancies. 53 In addition, Survivin is also involved in tumor cell resistance to radiation therapy.…”