Detection of anti‐<i>Brucella</i> antibodies in pinnipeds from the Antarctic territory

Retamal, Patricio; Abalos, Pedro; Blank, Olivia; Torres, Daniel

doi:10.1136/vr.146.6.166

Cited by 44 publications

(30 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although no Weddell seals tested positive for Brucella sp. or morbillivirus in our study, others have reported the presence of antibodies to these and other pathogens in Antarctic phocids (Bengtson et al 1991;Stenvers et al 1992;Retamal et al 2000). We did not detect leptospirosis in any of the Weddell seals we tested.…”

Section: Bacteria and Virusesmentioning

confidence: 54%

“…Recently, evidence of exposure to diseases generally associated with domestic animals and feral wildlife has been detected in Antarctic wildlife (Austin and Webster 1993;Olsen et al 1996;Gardner et al 1997;Retamal et al 2000;Foster et al 2002) and this has generated concern and debate regarding the risks of disease introduction to Antarctic wildlife. Antibodies to viruses that have caused large die-offs in phocids in other areas of the world have been detected in Weddell seals (Bengtson et al 1991), and there is a historical report of a mass die-off of crabeater seals that may have had a viral etiology (Laws and Taylor 1957).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Health Assessment of Weddell Seals, Leptonychotes weddellii, in McMurdo Sound, Antarctica

Yochem¹,

Stewart²,

Gelatt³

et al. 2009

Health of Antarctic Wildlife

View full text Add to dashboard Cite

Section: Bacteria and Virusesmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Health Assessment of Weddell Seals, Leptonychotes weddellii, in McMurdo Sound, Antarctica

Yochem¹,

Stewart²,

Gelatt³

et al. 2009

Health of Antarctic Wildlife

View full text Add to dashboard Cite

“…Therefore, understanding of the timing of exposure to Brucella and antibody status conversion in pinnipeds is lacking. Serum from 1 of 3 dead pups and 3 of 11 adult female Antarctic fur seals (Arctocephalus gazella) were positive for Brucella antibodies on cELISA with the use of a threshold inhibition value of 30% (Retamal et al, 2000). The result from the pup, however, was borderline positive and, therefore, possibly doubtful.…”

Section: Discussionmentioning

confidence: 96%

Epizootiology of Brucella Infection in Australian Fur Seals

Lynch

Duignan

Taylor

et al. 2011

Journal of Wildlife Diseases

View full text Add to dashboard Cite

ABSTRACT:Novel members of the bacterial genus Brucella have recently emerged as pathogens of various marine mammal species and as potential zoonotic agents. We investigated the epizootiology of Brucella infection in Australian fur seals (Arctocephalus pusillus doriferus) by establishing demographic and temporal variations in antibody prevalence, attempting isolation of the causative agent, and determining whether this potential pathogen is involved in frequent abortions observed in this pinniped species. Two competitive enzyme-linked immunosorbent assays (cELISAs), an indirect ELISA, and a fluorescence polarization assay (FPA) were used to test sera for Brucella antibodies. The FPA and cELISA proved suitable for use in this species. Significant differences in antibody prevalence were found between age classes of seals sampled between 2007 and 2009 at one colony. Pups sampled at this site (n5134) were negative for Brucella antibodies by all serologic tests but 17 of 45 (38%) of juveniles were antibody-positive. Antibody prevalence in adult females was significantly higher than in juveniles (P50.044). . Inflammatory lesions suggestive of infectious agents were found in 14 of 39 aborted Australian fur seal pups, but pathologic changes were not uniformly consistent for Brucella infection. Culture and PCR investigations on fetal tissues were negative for Brucella. Culture and PCR on selected fresh or frozen tissues from 36 juvenile and adult animals were also negative. We suspect that the prevalence of active infection with Brucella in Australian fur seals is low relative to antibody prevalence.

show abstract

“…The host range for Brucella spp. has recently expanded to include marine mammals, and anti-Brucella antibodies have been detected in cetaceans and pinnipeds around Europe and North and South America as well as the Arctic Sea (Nielsen et al, 1996;Ross et al, 1996;Garner et al, 1997;Jepson et al, 1997;Tryland et al, 1999;Forbes et al, 2000;Retamal et al, 2000;Van Bressem et al, 2001;Ohishi et al, 2003).…”

mentioning

confidence: 99%

Antibodies to Brucella spp. in Pacific Bottlenose Dolphins from the Solomon Islands

Tachibana

Watanabe

Kim

et al. 2006

Journal of Wildlife Diseases

View full text Add to dashboard Cite

ABSTRACT:Brucella spp. have been recently isolated from a variety of marine mammals. Serum samples from 58 Pacific bottlenose dolphins (Tursipa aduncus) from the Solomon Islands were tested for antibodies to Brucella spp. by the tube agglutination test (TAT), enzymed-linked immunosorbent assay (ELISA), and immunoblotting. Anti-Brucella spp. antibodies were detected by TAT and ELISA in 31 and 40 of 58 samples, respectively. These results suggest that Pacific bottlenose dolphins from the Solomon Islands are infected with Brucella spp. or a Brucella-like organism.

show abstract

Detection of anti‐Brucella antibodies in pinnipeds from the Antarctic territory

Cited by 44 publications

References 8 publications

Health Assessment of Weddell Seals, Leptonychotes weddellii, in McMurdo Sound, Antarctica

Health Assessment of Weddell Seals, Leptonychotes weddellii, in McMurdo Sound, Antarctica

Epizootiology of Brucella Infection in Australian Fur Seals

Antibodies to Brucella spp. in Pacific Bottlenose Dolphins from the Solomon Islands

Contact Info

Product

Resources

About