Detection of anaerobic bacteria in blood cultures by lysis filtration

Heimdahl, Anders; Josefsson, Kenneth; Konow, L. von; Ce, Nord

doi:10.1007/bf02148693

Cited by 19 publications

(26 citation statements)

References 6 publications

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“…The nature of the organisms are typical of those obtained from odontogenic bacteraemia. 13,14 The bacteraemia rate in the cardiac group is similar to that from other studies where antibiotic prophylaxis was administered orally, either in children, or adults. 14,15 This is surprising considering that IV antibiotics should achieve very high blood levels at the time of dental treatment.…”

Section: Discussionsupporting

confidence: 79%

Intravenous antibiotic regimens and prophylaxis of odontogenic bacteraemia

Roberts

Holzel

2002

Br Dent J

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Section: Discussionsupporting

confidence: 79%

Intravenous antibiotic regimens and prophylaxis of odontogenic bacteraemia

Roberts

Holzel

2002

Br Dent J

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“…The ability of LyF to estimate the intensity of bacteremia was confirmed with values ranging from 1 to 28 CFU/ml following the extraction of a single tooth. This compares well with the data for intensity in adults, for which the values were up to 43 CFU/ml (16), a mean of 3.8 CFU/ml (14), and a mean of 21 CFU/ml (17), respectively.…”

Section: Discussionsupporting

confidence: 87%

“…Preliminary work has shown that the technique of lysis filtration (LyF) appears to be an effective method of estimating the intensity of bacteremia following dental extractions in adults (14,15). There are no data available on the use of LyF with children.…”

mentioning

confidence: 99%

Comparison of Lysis Filtration and an Automated Blood Culture System (BACTEC) for Detection, Quantification, and Identification of Odontogenic Bacteremia in Children

Lucas¹,

Lytra²,

Hassan³

et al. 2002

J Clin Microbiol

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Lysis filtration (LyF) was compared with BACTEC PAEDS PLUS in estimating the prevalence of, and sensitivity for, detection of odontogenic bacteremia. Both real bacteremia and simulated bacteremia (seeded blood or saline samples) were assessed to determine the validity of LyF in estimating bacteremia. The simulated bacteremia was also used to assess the reliability of LyF to estimate intensity of bacteremia in CFU per milliliter of blood. Reference organisms were used to assess the abilities of LyF and BACTEC to isolate known oral streptococci. There was no difference in the number of CFU per milliliter of seeded saline, seeded blood, and drop cultures of the organisms plated directly onto agar. Blood cell volume had a negligible effect on the yield of organisms for simulated bacteremia. When LyF and BACTEC were compared, the time to detection of bacteremia was always significantly shorter for BACTEC. For aerobic cultures, these times were 43.7 and 9.6 h, respectively (P < 0.01). For anaerobic cultures, these times were 45.1 and 9.9 h, respectively (P < 0.01). These differences occurred as well for bacteremia following the extraction of a single tooth, with LyF and BACTEC aerobic cultures taking 78 and 30.5 h, respectively (P < 0.0001). For anaerobic cultures, the times were 90.8 and 45 h, respectively (P < 0.0004). A preextraction bacteremia was detected on 2.1% of occasions with BACTEC compared to 31% of occasions with LyF (P < 0.05) The use of LyF was an effective and reliable means of estimating the intensity of pre-and postextraction bacteremia. The values were 3.6 and 5.9 CFU/ml, respectively (P < 0.4729), and the difference was not statistically significant. In summary, BACTEC is quicker than LyF, but less sensitive. LyF provides additional important information in estimating the intensity of bacteremia.

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“…Vacuum filtration was done using a 0.45-µm pore-size filter system (Millipore Corp, Billerica, La., USA). This system has been evaluated for aerobic [10] and anaerobic bacteria [11]. After filtration, the filters were placed onto brain-heart infusion agar (Difco Laboratories, Detroit, Mich., USA) plates supplemented with 5% horse blood for anaerobic incubation at 37°C for up to 10 days, before the samples were judged negative.…”

Section: Methodsmentioning

confidence: 99%

Bacteraemia in Patients with Hidradenitis Suppurativa Undergoing Carbon Dioxide Laser Surgery: Detection and Quantification of Bacteria by Lysis-Filtration

et al. 2006

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Background: Hidradenitis suppurativa (HS) is a cicatrising and persistent disease of apocrine gland-bearing areas in adults. The severity of this condition varies from a few suppurating lesions to widespread, disabling disease. The aetiology is obscure, but suggested contributory factors include a genetic predisposition, comedones occluding the pilosebaceous apparatus, bacterial infections, and hormonal factors. Treatment consists mainly of surgery, while medical therapies serve principally as adjunct therapy. Objectives: The aim of the study was to determine the number and type of bacteria circulating in the bloodstream in patients with HS undergoing surgical treatment with a carbon dioxide laser stripping-secondary intention technique. Methods: Twenty-one patients (20 females and 1 male, mean age 36, range 20–55 years) were included in the study. One blood sample (8.3 ml) was taken before surgery, one during the operation and the last one 10 min after surgery. Five healthy persons (all females, mean age 36, range 23–48 years) not undergoing any operation were used as the controls. The blood was cultured by a lysis-filtration technique which had been shown to be very sensitive. Since the filter catches the microorganisms and colonies are formed during culturing, the number of bacteria in the samples is easily determined. Results: In 6 patients, all samples were negative, which indicates that the method of surgery itself caused no spread of bacteria from the lesions. Bacterial growth in the first blood sample was found in 9 patients, from the second sample in 10 and from the third one in 6. In 1 patient, bacteria were detected in three samples. At least 12 bacterial species were identified. The dominating bacteria were coagulase-negative staphylococci of which most were subtyped as Staphylococcus warneri. Among the anaerobic microorganisms, Propionibacterium acnes and P.granulosum were the most frequently isolated bacteria. The bacterial findings in the blood samples accord well with the results from a previous study in which cultures were taken from the deep parts of the HS lesions. In the 5 controls, no microbial growth was detected. Conclusion: The carbon dioxide laser stripping technique caused no additional spread of bacteria into the bloodstream. The evaluation of cultures containing microorganisms from normal skin flora is controversial. Since the bacteria encountered in this study are in close agreement with the findings in cultures from the deeper parts of HS lesions they seem to be relevant. The growth of bacteria in the first blood sample taken before surgery may indicate that some of these patients have bacteria continuously circulating in their blood.

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Detection of anaerobic bacteria in blood cultures by lysis filtration

Cited by 19 publications

References 6 publications

Intravenous antibiotic regimens and prophylaxis of odontogenic bacteraemia

Intravenous antibiotic regimens and prophylaxis of odontogenic bacteraemia

Comparison of Lysis Filtration and an Automated Blood Culture System (BACTEC) for Detection, Quantification, and Identification of Odontogenic Bacteremia in Children

Bacteraemia in Patients with Hidradenitis Suppurativa Undergoing Carbon Dioxide Laser Surgery: Detection and Quantification of Bacteria by Lysis-Filtration

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