Detection of alkaline phosphatase/immunoglobulin complexes.

Buttery, J E; Milner, C.R.; Nenadovic, P; Pannall, P R

doi:10.1093/clinchem/26.11.1620

Cited by 20 publications

(12 citation statements)

References 10 publications

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“…Reported macro-ALP were bound to IgG [6,7] and to a lesser extent IgA [8,9]. e ALP is usually of liver and bone origin or both [10]. Gel filtration analysis of the macro-ALP suggested that the results will be consistent with equimolar complexes of IgG-ALP or a complex made up of two molecules of IgG with one of ALP or one molecule of IgG and two molecules of ALP [6].…”

Section: Discussionmentioning

confidence: 95%

“…is method will not detect complexes to immunoglobulins other than IgG so has limitations [10]. In a further case study, Nagakawa et al [13] reported an intestinal ALP-IgG complex in which the IgG had the ability to inhibit ALP in a patient's serum.…”

Section: Discussionmentioning

confidence: 99%

“…Macro-ALP has been reported in patients with ulcerative colitis [11], carcinoma of prostate [10], bronchopneumonia and recurrent infections [9], and liver cirrhosis and cholestasis [6]. Atypical ALP with posttranslational modification has been described in a patient with Hodgkin's disease [14].…”

Section: Discussionmentioning

confidence: 99%

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Persistent Increase in Serum Alkaline Phosphatase in a Patient with Monoclonal Gammopathy of Undefined Significance

Ramasamy

2020

Case Reports in Hematology

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We report the finding of alkaline phosphatase-immunoglobulin complex (macro-alkaline phosphatase (macro-ALP)) in a patient with persistently increased ALP activity. The identification of macro-ALP is important to rule out pathological causes of increased ALP activity and to avoid unnecessary diagnostic investigation. The patient was subsequently diagnosed with vitamin D deficiency, gallstone pancreatitis, and monoclonal gammopathy of undefined significance (MGUS). Macro-ALP can coexist with disease that can increase serum ALP activity. We report, for the first time, a case of macro-ALP in a patient with a monoclonal protein (M-protein).

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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Persistent Increase in Serum Alkaline Phosphatase in a Patient with Monoclonal Gammopathy of Undefined Significance

Ramasamy

2020

Case Reports in Hematology

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“…The baseline values for serum-biochemical parameters were determined before the commencement of the experiment, according to standard procedures (Barham and Trinder, 1972;Patton and Crouch, 1977;Buttery et al, 1980;Grant, 1987). Blood samples were collected via the wing vein, using 25 gauge sterile hypodermic needles and syringes and then kept in a slanted position for serum to settle.…”

Section: Determination Of Serum Biochemical Parametersmentioning

confidence: 99%

“…This was done using the method of Buttery et al (1980). 500µl of blank; standard and sample was pipette into test tube and incubated at 37°C for 10 minutes, after which 2500µl of alkaline phosphatase colour developer was added into each of the test tubes, mixed and then the absorbance was read using a spectrophotometer at wavelength of 590nm.…”

Section: Assay For Serum Alkaline Phosphatase (Alp)mentioning

confidence: 99%

Serum biochemical changes in Japanese quail (coturnix coturnix japonica) experimentally infected with salmonella enterica serovar gallinarum

I.J¹,

J.O.O²,

S.B³

et al. 2015

Afr. J. Cell. Path

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Aim: To determine the serum biochemical changes in Japanese quail (Coturnix coturnix japonica) experimentally infected with Salmonella enterica serovar Gallinarum Methods: A total of 160 (108 males and 52 females) Japanese quails (Coturnix coturnix japonica) were used for the experiment. Four weeks old quails were randomly selected and assigned into four groups (A, B, C and D) of forty quails each. Groups A, B and C were infected with Salmonella enterica serovar Gallinarum per os at the dose of 10 6 ,10 4 and 10 2 , respectively, while group D served as the control. Sera were used for the determination of biochemical parameters. Results: There were significant changes (P<0.05) in serum glucose, total protein, albumin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and urea concentrations between infected groups and the control group. Significant changes (P<0.05) in these biochemical parameters were also observed post-infection compared to pre-infection within each infected group. Conclusion: FT in quails results in severe multi organ dysfunction and damage as demonstrated by the serum biochemical changes noticed suggestive of immediate cause of mortality in FT infection.

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Antigens in immune complexes from patients with breast cancer

Gilead¹,

Hatzubai²,

Sulitzeanu³

1982

Cancer Immunol Immunother

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Sera and effusion fluids of patients with breast cancer (BC) contain immune complexes (IC). Antigens present in these complexes were isolated as follows: a pool of effusions from patients with BC was fractionated with ammonium sulfate. The proteins precipitating at 40% saturation were further fractionated by filtration through a Sephadex G-200 column. The material recovered in the first peak (molecules larger than monomeric IgG) was brought to pH 3.0 to dissociate the IC, and the mixture was filtered through a column of Sephacryl S-300 at pH 3.0. Proteins smaller than monomeric IgG were collected, radioiodinated, and used as antigens (125Ag) to search for corresponding antibodies in sera of patients with BC (BCS) and of healthy individuals (NHS). 125Ag was reacted with the sera and the immune complexes obtained were precipitated with an antiserum to human Ig and analyzed by SDS-polyacrylamide gel electrophoresis followed by autoradiography. Both NHS and BCS contained antibodies against two antigens; one of these appeared as a strong band of 17KD, the other as a doublet of approximately 25KD. It is concluded that some of the proteins in the IC from patients with BC are auto-antigens. No BC-specific antigens were identified.

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Detection of alkaline phosphatase/immunoglobulin complexes.

Cited by 20 publications

References 10 publications

Persistent Increase in Serum Alkaline Phosphatase in a Patient with Monoclonal Gammopathy of Undefined Significance

Persistent Increase in Serum Alkaline Phosphatase in a Patient with Monoclonal Gammopathy of Undefined Significance

Serum biochemical changes in Japanese quail (coturnix coturnix japonica) experimentally infected with salmonella enterica serovar gallinarum

Antigens in immune complexes from patients with breast cancer

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