Detection of aflatoxins (G1–2, B1–2), sterigmatocystin, citrinine and ochratoxin A in samples contaminated by microbes

Tuomi, Tapani; Johnsson, Tom; Hintikka, Eeva-Liisa; Reijula, Kari

doi:10.1039/b103790k

Cited by 32 publications

(20 citation statements)

References 17 publications

(26 reference statements)

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“…A HPLC-MS analysis method for determining citrinin was established (Tuomi, Johnsson, Hintikka, & Reijula, 2001), which included extraction, sample pretreatment and reversed-phase HPLC separation with MS identification and quantification using electrospray ionization on a quadrupole ion trap mass analyser (ESI-MS-MS). Mass spectral analysis was performed on a Finnigan LCQ fitted with an electrospray ionisation (ESI) probe in the positive ion mode.…”

Section: Lc-ms Techniquementioning

confidence: 99%

Review on the qualitative and quantitative analysis of the mycotoxin citrinin

et al. 2006

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Section: Lc-ms Techniquementioning

confidence: 99%

Review on the qualitative and quantitative analysis of the mycotoxin citrinin

et al. 2006

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“…Beside the development of databases dealing with qualitative LC/MS of mycotoxins (Nielsen and Smedsgaard 2003), this has led to early quantitative methods for the simultaneous determination of Aspergillus and Penicillium mycotoxins in building materials (Tuomi et al 2001) and in an artificial food matrix (Rundberget and Wilkins 2002). While the former method suffered from low recoveries of some analytes, excellent accuracy and precision were obtained in the latter case through use of a de-fatting step applied to the raw extract, and use of matrixmatched calibration to compensate for matrix effects.…”

Section: Multi-mycotoxin Methodsmentioning

confidence: 99%

Mycotoxin analysis: An update

Krska¹,

Schubert-Ullrich²,

Molinelli³

et al. 2008

Food Additives & Contaminants: Part A

310

215

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Mycotoxin contamination of cereals and related products used for feed can cause intoxication, especially in farm animals. Therefore, efficient analytical tools for the qualitative and quantitative analysis of toxic fungal metabolites in feed are required. Current methods usually include an extraction step, a clean-up step to reduce or eliminate unwanted co-extracted matrix components and a separation step with suitably specific detection ability. Quantitative methods of analysis for most mycotoxins use immunoaffinity clean-up with high-performance liquid chromatography (HPLC) separation in combination with UV and/or fluorescence detection. Screening of samples contaminated with mycotoxins is frequently performed by thin layer chromatography (TLC), which yields qualitative or semi-quantitative results. Nowadays, enzyme-linked immunosorbent assays (ELISA) are often used for rapid screening. A number of promising methods, such as fluorescence polarization immunoassays, dipsticks, and even newer methods such as biosensors and non-invasive techniques based on infrared spectroscopy, have shown great potential for mycotoxin analysis. Currently, there is a strong trend towards the use of multi-mycotoxin methods for the simultaneous analysis of several of the important Fusarium mycotoxins, which is best achieved by LC-MS/MS (liquid chromatography with tandem mass spectrometry). This review focuses on recent developments in the determination of mycotoxins with a special emphasis on LC-MS/MS and emerging rapid methods.

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“…In addition, mycotoxins have been detected in moldy building materials (Nielsen et al, 1998(Nielsen et al, , 1999Tuomi et al, 2000) and in the dust of ventilation ducts (Smoragiewicz et al, 1993). When sampled, mycotoxins can be determined by chromatographic methods, such as thin-layer chromatography, gas chromatography/mass spectrometry, and high-performance liquid chromatography (Nielsen and Thrane, 2001;Tuomi et al, 2001). The toxicity of indoor-related microbes may depend on the material on which the organism is growing.…”

Section: Analysis Of Biological Propertiesmentioning

confidence: 99%

Analysis of Chemical and Biological Properties

et al. 2003

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Electron Microscopy-Based Microanalysis of Airborne Particles IntroductionElectron microscopy (EM), namely transmission and scanning, is the most common technique used for particle analysis, at both the morphological and chemical levels; it is assumed that the reader is familiar with the basic principles of electron microscope design and operation and only the practical applications will be considered. In terms of particle identification based on morphology, transmission electron microscopy (TEM) is the more robust system, especially when dealing with airborne particles that are submicron in size. TEM will yield information on the ultrastructure, providing a two-dimensional interpretation of a particle. In contrast, scanning electron microscopy (SEM) is more limited to airborne particles that are microns in size and will produce information about a particle's surface structure or a three-dimensional interpretation.The added bonus of EM is its capacity to determine the elemental composition of airborne particles, at both the individual and bulk particle levels. Several analytical techniques are available for the elemental characterization of specimens within the electron microscope (e.g. X-ray analysis, Auger electron spectroscopy, and electron energy loss spectroscopy). For all such techniques, a signal is produced by either direct electron bombardment, or by interaction with auxiliary excitation sources (e.g. X-rays) and collected in appropriate detectors (i.e., an energy dispersive spectrometer [EDS]), to yield specific information about the elemental features of a specimen. In the case of particle analysis, elemental characterization is best achieved by means of X-ray detection using a technique more commonly known as electron probe X-ray microanalysis (EPXMA). X-rays within the electron microscope are generated by the interaction of the electron beam with individual atoms in the specimen (e.g. characteristic X-rays). These X-rays carry information about the elemental composition of the specimen in the region that is being irradiated and consequently provide a means of correlating structural information with elemental content. Therefore, in theory, X-ray microanalysis is possible using any electron microscope fitted with a suitable detector system, and this is generally true for qualitative X-ray microanalysis. Quantitative X-ray microanalysis is a 105 2.4

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Detection of aflatoxins (G1–2, B1–2), sterigmatocystin, citrinine and ochratoxin A in samples contaminated by microbes

Cited by 32 publications

References 17 publications

Review on the qualitative and quantitative analysis of the mycotoxin citrinin

Review on the qualitative and quantitative analysis of the mycotoxin citrinin

Mycotoxin analysis: An update

Analysis of Chemical and Biological Properties

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