Detection of aerobic bacterial pathogens associated with early embryonic death in pregnant New Zealand female Rabbits in Egypt

Abstract

Background and Aim: Rabbits are a highly sensitive species and susceptible to various bacterial pathogens that may be causative agents for early embryonic death. This study aimed to explore the administration of different bacterial agents in does suffering from early embryonic death. Furthermore, identification of genes associated with virulence was performed to identify the phenotypic and genotypic antimicrobial resistance patterns that may increase the virulence of pathogens and lead to early embryonic death. Materials and Methods: We isolated and identified bacterial agents in 106 samples from live and dead female rabbits that had undergone early embryonic death, including liver and intestine tissue, aborted fetuses, discharges, and vaginal swabs. Conventional polymerase chain reaction (PCR) was conducted to confirm the identity of the isolated bacterial strains and their virulence. Moreover, antibiotic resistance was studied phenotypically and genotypically. Results: We isolated Escherichia coli, Salmonella, Staphylococcus aureus, Pasteurella multocida, and Listeria monocytogenes. PCR confirmed typical identification except in P. multocida, which was confirmed as Gallibacterium spp. in some cases. The final percentage of detection was 34%, 30.2%, 16.9%, 13.2%, and 11.3%, respectively. Virulence properties were investigated using different designated genes. All Salmonella strains harbored invA, stn, avrA, and ompf genes, while the sopE gene was identified in 31.25%. E. coli strains harboring the iss gene lacked the shiga toxin (stx1) gene. L. monocytogenes and S. aureus strains harbored the hemolysin gene (66.7% and 33.4%, respectively). Multidrug resistance was detected phenotypically and genotypically in most strains. Each bacterial pathogen had a different antibiotic resistance profile. Conclusion: Multiple bacterial species may contribute to early embryonic death in does. Furthermore, the combined infection could be the main cause of early embryonic death. Thus, monitoring programs should bear this in mind and focus on the early detection of these bacterial agents in female rabbits to avoid embryonic death.

Detection of virulence, specific genes and antibiotic resistance of isolated Salmonella spp. strains from rabbits infected with salmonellosis

“…All strains tested carried the in-vA-specific gene (PCR products of 284 bp in size), confirming that these isolates are Salmonella spp. The results were corroborated by other studies [3,27,28].…”

“…Previously, the stn gene was present in all strains of Salmonella spp. isolated from animals [28,29]. fimA is the gene encoding fimbriae in Salmonella spp.…”

Mortalities Among Young Rabbits: Impact of Maternal Behavior and Bacterial Causes

“…Bacteriological tests proved that S. aureus was one of the most common pathogens in dead rabbits, similar to the results obtained by Abd El Gwad et al ( 2004) who detected S. aureus in breeders, causing mastitis, uterine irritation, and abscess or dermatitis in growing rabbits, resulting in the death of young rabbits and newborns. As well as Roshdy et al (2021) mention that S. aureus is one of the important pathogens causing the death of rabbits in different inspection farms. The widespread of S. aureus is due to its frequent presence in the environment, and nasal carriers play an important role as a source of infection for S. aureus transmission (Becker and Von Eiff, 2011).…”