Detection of adenovirus and initiation of apoptosis in hepatocellular carcinoma cells after ad- p53 treatment

Mitry, Ragai R.; Sarraf, Catherine; Havlík, R; Habib, Nagy

doi:10.1053/he.2000.5631

Cited by 42 publications

(28 citation statements)

References 20 publications

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“…Therapeutic genes can be delivered to the nucleus of cells to repair or substitute defective genes andproduce therapeutic proteins (Ouma, Jonas et al 2012). Suicide genes can be delivered to the nucleus of cancer cells where the gene encodes a protein product that causes cellular apoptosis (Mitry, Sarraf et al 2000;Fillat, Carrio et al 2003). Antisense oligonucleotides or siRNA can be delivered to the nucleus of a specific target cell to block the gene expression and silence a problematic gene (Kang, Kim et al 2000;Li, Fu et al 2005).…”

mentioning

confidence: 99%

Evaluation of Cell-Penetrating Peptide/Adenovirus Particles for Transduction of CAR-Negative Cells

Nigatu

Vupputuri

Flynn

et al. 2013

Journal of Pharmaceutical Sciences

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Linear low‐density polyethylene (LLDPE), based on butene‐1 or hexene‐1, was irradiated with γ‐rays under vacuum or in the presence of air. The study focused on the influence of the dose rate and the γ‐dose on the thermal properties of LLDPE. Differential scanning calorimetry, thermogravimetric analysis (TGA), and TGA/FTIR techniques were used to address the thermal behavior as a result of γ‐irradiation. During this irradiation, competition between crosslinking and scission reactions, subsequent to oxidation reactions, occurred in the polymeric material, which strongly depends on the experimental conditions. A decrease of the crystallinity for γ‐irradiated samples was observed in particular for samples irradiated under vacuum. This observation may be explained by increased hindrance of segment mobility due to crosslinking reactions that prevent crystal growth. TGA investigations revealed an enhancement of the thermal stability for samples irradiated under vacuum but not for those irradiated in air. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 100: 2790–2795, 2006

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mentioning

confidence: 99%

Evaluation of Cell-Penetrating Peptide/Adenovirus Particles for Transduction of CAR-Negative Cells

Nigatu

Vupputuri

Flynn

et al. 2013

Journal of Pharmaceutical Sciences

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“…29 Hepatocyte attachment was assessed using the sulforhodamine B (SRB) assay. 30 Albumin and urea synthesis were assessed for cells cultured in collagencoated 24-well plates (3.0 Â 10 5 cells/well). Albumin production was quantified in supernatant using human or rat albumin enzyme-linked immunosorbent assay (ELISA) quantitation kits (Bethyl Laboratories, Montgomery, TX, USA).…”

Section: Plated Hepatocyte Viability and Functionmentioning

confidence: 99%

Cryopreservation of Hepatocyte Microbeads for Clinical Transplantation

et al. 2017

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Intraperitoneal transplantation of hepatocyte microbeads is an attractive option for the management of acute liver failure. Encapsulation of hepatocytes in alginate microbeads supports their function and prevents immune attack of the cells. Establishment of banked cryopreserved hepatocyte microbeads is important for emergency use. The aim of this study was to develop an optimized protocol for cryopreservation of hepatocyte microbeads for clinical transplantation using modified freezing solutions. Four freezing solutions with potential for clinical application were investigated. Human and rat hepatocytes cryopreserved with University of Wisconsin (UW)/10% dimethyl sulfoxide (DMSO)/5% (300 mM) glucose and CryoStor CS10 showed better postthawing cell viability, attachment, and hepatocyte functions than with histidine-tryptophan-ketoglutarate/10% DMSO/5% glucose and Bambanker. The 2 freezing solutions that gave better results were studied with human and rat hepatocytes microbeads. Similar effects on cryopreserved microbead morphology (external and ultrastructural), viability, and hepatocyte-functions post thawing were observed over 7 d in culture. UW/DMSO/glucose, as a basal freezing medium, was used to investigate the additional effects of cytoprotectants: a pan-caspase inhibitor (benzyloxycarbonyl-Val-Ala-DL-Asp-fluoromethylketone [ZVAD]), an antioxidant (desferoxamine [DFO]), and a buffering and mechanical protectant (human serum albumin [HSA]) on RMBs. ZVAD (60 mM) had a beneficial effect on cell viability that was greater than with DFO (1 mM), HSA (2%), and basal freezing medium alone. Improvements in the ultrastructure of encapsulated hepatocytes and a lower degree of cell apoptosis were observed with all 3 cytoprotectants, with ZVAD tending to provide the greatest effect. Cytochrome P450 activity was significantly higher in the 3 cytoprotectant groups than with fresh microbeads. In conclusion, developing an optimized cryopreservation protocol by adding cytoprotectants such as ZVAD could improve the outcome of cryopreserved hepatocyte microbeads for future clinical use.

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“…However, transfer of wild-type p53 gene has been extensively tested in HCC regardless of their p53 status. In vitro, overexpression of wild-type p53 in some HCC cell lines has been shown to induce cell apoptosis [47] or inhibit cell growth [48]. Intra-tumoral injection of Ad-p53 in a subcutaneously inoculated HCC [49] or repeated intra-arterial injection of Ad-p53 in an orthotopically implanted rat HCC model [50] was shown to inhibit tumor growth.…”

Section: Apoptosis-mediated Cell Deathmentioning

confidence: 99%

Gene Therapy Strategies for Hepatocellular Carcinoma

Hwang

2006

J Biomed Sci

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SummaryHepatocellular carcinoma (HCC) is one of the most frequent cancers worldwide. Effective therapy to this cancer is currently lacking, creating an urgent need for new therapeutic strategies for HCC. Gene therapy approach that relies on the transduction of cells with genetic materials, such as apoptotic genes, suicide genes, genes coding for antiangiogenic factors or immunomodulatory molecules, small interfering RNA (siRNA), or oncolytic viral vectors, may provide a promising strategy. The aforementioned strategies have been largely evaluated in the animal models with HCC or liver metastasis. Due to the diversity of vectors and therapeutic genes, being used alone or in combination, gene therapy approach may generate great beneficial effects to control the growth of tumors within the liver.

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Detection of adenovirus and initiation of apoptosis in hepatocellular carcinoma cells after ad- p53 treatment

Cited by 42 publications

References 20 publications

Evaluation of Cell-Penetrating Peptide/Adenovirus Particles for Transduction of CAR-Negative Cells

Evaluation of Cell-Penetrating Peptide/Adenovirus Particles for Transduction of CAR-Negative Cells

Cryopreservation of Hepatocyte Microbeads for Clinical Transplantation

Gene Therapy Strategies for Hepatocellular Carcinoma

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