Detection of A2A2 genotype of beta casein protein (<i>CSN2</i>) gene in local, exotic and cross bred cattle in Pakistan

Ayaz,; Suhail, Syed Muhammad; Ahmad, Ijaz; Zeb, Muhammad Tariq; Khan, Rajwali; Ijaz, Asim; Ahmad, Iftikhar; Riaz, Muhammad Hasnanin; Ali, Farhad; Khan, Khalid Ali; Khan, Aitbar

doi:10.1080/10495398.2022.2031204

Cited by 5 publications

(2 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, Patel et al (2019) identified three different genotypes as A1A1 (0.01), A1A2 (0.34) and A2A2 (0.65) in Gir cattle of India while the corresponding A1 allele frequency (0.18) was found much higher than in the earlier reports. In addition, previous reports mentioned the existence of low frequent A1 allele in different zebu populations that ranged between 0.00 and 0.11 in Ongole, Malanad Gidda, Kherigarh, Kasargod, Achai and Lohani (Mishra et al 2009;Ganguly et al 2013;Ramesha et al 2016;Ayaz et al 2022) that support the present findings. Besides, Mishra et al (2009) reported the absence of A1 allele in the zebu breeds of Gir, Sahiwal, Red Sindhi, Kangayam, Nimari, Red Kandhari, Malvi, Amrit Mahal, Kankrej, Hariana, Mewati, Tharparker and Rathi and it is inconsistent with this study.…”

Section: Sequence Characterization Of Csn2 Genesupporting

confidence: 89%

“…Likewise, Malarmathi et al (2014) reported the proportion of A1A1, A1A2 and A2A2 genotypes to be 0.17, 0.46 and 0.37, respectively, in HF crossbred cattle of Tamil Nadu, India. The frequencies of the three genotypes were 0.56 (A1A1), 0.26 (A1A2) and 0.18 (A2A2) in Sahiwal × Friesian crossbreds of Pakistan (Ayaz et al 2022). The A1 allele frequency 0.46 was found in Vechur crossbred cattle (Muhammed and Stephen 2012) and is consistent with this finding.…”

Section: Genotyping Of A1 and A2 Allelic Variantssupporting

confidence: 80%

See 1 more Smart Citation

Molecular characterization and A1/A2 genotyping of casein beta gene in zebu and crossbred cattle of Bangladesh

Pabitra¹,

Maruf²,

Mony³

et al. 2022

CZECH J ANIM SCI

View full text Add to dashboard Cite

Casein beta (CSN2) is the most explored gene in cattle due to its potential impact on human health. Here, we investigated the entire coding sequence of CSN2 gene except the last two codons for detection of polymorphisms in different cattle populations of Bangladesh as well as genotyping of A1 and A2 allelic variants using a newly developed allele specific polymerase chain reaction (AS-PCR) based the genotyping protocol. Five primer pairs were used to amplify the coding sequences of CSN2 gene. This study included a total of 258 DNA samples from six Bangladeshi zebu populations and one Holstein Friesian (HF) derived crossbred cattle population. Sequence analysis detected five nonsynonymous mutations in the coding sequence of CSN2 gene that defined five allelic variants as A1, A2, B, F and I. In addition, substitution of GTA (Val) by GCA (Ala) at position 197 resulted in an undefined allele in the zebu cattle population of Bangladesh that has not yet been reported elsewhere. Like other Bos indicus cattle populations, A2 allele is predominant in the studied zebu populations. The mean frequencies of A1A1, A1A2 and A2A2 genotypes were 0.02, 0.16 and 0.82, respectively, in zebu cattle populations while the corresponding allele frequencies were A1 (0.10) and A2 (0.90). In opposite, the aforesaid genotype frequencies were 0.14 (A1A1), 0.50 (A1A2) and 0.36 (A2A2) in the HF crossbred population with allele frequencies of 0.39 (A1) and 0.61 (A2). The adopted AS-PCR method was found cost-effective, rapid and had high specificity for genotyping of A1 and A2 allelic variants. Altogether, this study provides information for the selection of desired zebu and crossbred individuals in order to produce premium quality milk as well as to design a breeding plan in the crossbreeding program.

show abstract

Section: Sequence Characterization Of Csn2 Genesupporting

confidence: 89%

Section: Genotyping Of A1 and A2 Allelic Variantssupporting

confidence: 80%