Detection of a Possible Bioterrorism Agent, Francisella sp., in a Clinical Specimen by Use of Next-Generation Direct DNA Sequencing

Kuroda, Makoto; Sekizuka, Tsuyoshi; Shinya, Fumiaki; Takeuchi, Fumihiko; Kuriyama, Takayuki; Sata, Tetsutaro; Asano, Shigeyuki

doi:10.1128/jcm.06715-11

Cited by 42 publications

(29 citation statements)

References 8 publications

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“…holarctica. This conclusion is consistent with recent reports of tularemia in which case definitions contained compatible clinical symptoms and positive F. tularensis real-time PCR or DNA sequencing (Maurin et al, 2011;Kuroda et al, 2012).…”

Section: Resultssupporting

confidence: 92%

A case of Francisella tularensis subspecies holarctica in China

Wang¹,

Qiao

Cao

et al. 2015

Ticks and Tick-borne Diseases

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Section: Resultssupporting

confidence: 92%

A case of Francisella tularensis subspecies holarctica in China

Wang¹,

Qiao

Cao

et al. 2015

Ticks and Tick-borne Diseases

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“…Pus samples were aspirated from the enlarged lymph node of the patient 44 days after disease onset (9). The remaining hare carcass was recovered from a plastic trash box placed outside the patient's house 65 days after skinning and butchering the animal, and the decomposed muscles were subjected to DNA extraction.…”

mentioning

confidence: 99%

“…holarctica, which is endemic to Japan, by PCR ( Fig. 1) and direct DNA sequencing performed using the Illumina GAIIx system (Illumina, San Diego, Calif., USA) (9).…”

mentioning

confidence: 99%

Identification of the Source of <i>Francisella tularensis</i> Infection by Multiple-Locus Variable-Number Tandem Repeat Analysis

et al. 2013

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SUMMARY: Tularemia is a zoonotic disease caused by Francisella tularensis. Most patients in Japan have reportedly acquired such infections through direct contact with infected Japanese hares. We recently encountered a patient who contracted tularemia after skinning and butchering a dead hare. Because the remains of the hare were available, we attempted to determine whether the patient actually contracted infection by handling the carcass. F. tularensis-specific sequences were successfully amplified by PCR from the patient specimens as well as from the remnants of discarded hare carcass. PCR amplification of the ISFtu2 and RD1 regions indicated infection by F. tularensis subsp. holarctica, which was considered as a prevalent strain in Japan. Furthermore, high-resolution multiple-locus variablenumber tandem repeat analysis (MLVA) showed that the combination of repeat numbers in sequences from the patient and hare were indistinguishable, thus indicating that the patient had been infected with F. tularensis strain that had also infected the hare. These findings demonstrated that MLVA is a useful epidemiological investigational tool to identify possible sources of certain zoonotic diseases such as tularemia.

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“…NGS has also been used to detect strain-specific polymorphism in B. anthracis and Y. pestis 47 . Next-generation direct DNA sequencing could detect F. tularensis in a human abscess sample of unknown etiology 48 . Recently, targeted amplification approach has been described for detection of bio-threat agents by NGS 49 .…”

Section: Next Generation Sequencingmentioning

confidence: 99%

Biological Warfare Agents and their Detection and Monitoring Techniques (Review Paper)

Pal¹,

Sharma²,

Sharma³

et al. 2016

Def. Sc. Jl.

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Recently, threat from biological warfare agents (BWAs) has emerged as the foremost national and global security challenge because of their simple and cheap production, easy dispersal, complicated detection, expensive protection and psychological, economical and social impact. Early detection and identification of BWAs during intentional biological event is essential to initiate corrective emergency responses for management of such incidents. Efforts are being made across the globe for development of state of the art technologies and systems for detection and identification of BWAs. However, till date there is no single system which can detect all the bio-threat agents. In the present review, we describe the currently available techniques and systems for detection and identification of these agents. The basic identification techniques including biological culture, immunological methods, nucleic acid based detection, MALDI-TOF MS, cellular fatty acid profiling and flow cytometry based detection are presented. Detection of BWAs with bio-sensors, surface plasmon resonance, biological detectors, and stand-off detection systems is also summarized. However, despite of availability of several techniques and tools, no full proof system is available for detection/identification of all the BWAs.

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Detection of a Possible Bioterrorism Agent, Francisella sp., in a Clinical Specimen by Use of Next-Generation Direct DNA Sequencing

Cited by 42 publications

References 8 publications

A case of Francisella tularensis subspecies holarctica in China

A case of Francisella tularensis subspecies holarctica in China

Identification of the Source of <i>Francisella tularensis</i> Infection by Multiple-Locus Variable-Number Tandem Repeat Analysis

Biological Warfare Agents and their Detection and Monitoring Techniques (Review Paper)

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