Detection of a neonatal human rotavirus strain with VP4 and NSP4 genes of porcine origin

Mascarenhas, Joana D’Arc Pereira; Leite, José Paulo Gagliardi; Lima, Jackson Cordeiro; Heinemann, Marcos Bryan; Oliveira, Darleise S.; Araújo, Irene Trigueiros; Soares, Luana da Silva; Gusmão, Rosa Helena Porto; Gabbay, Yvone Benchimol; Linhares, Alexandre C.

doi:10.1099/jmm.0.46635-0

Cited by 35 publications

(27 citation statements)

References 59 publications

(53 reference statements)

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“…The introduction of a new human-animal reassortant RVs strain into the human population could have an impact on the spread of rotavirus disease and also on prevention measures (STEYER et al, 2008). This study also revealed (data not shown) that strain PORV6 had 96,4% amino acid identity with Brazilian strain NB-150, a human strain previously isolated by Mascarenhas et al (2007) from a newborn with diarrhea who lived in the outskirts of Belém, State of Pará, Brazil, that reinforce the hypothesis that interspecies transmission may occur naturally, without loss of virulence (VARGHESE et al, 2004).…”

Section: Discussionsupporting

confidence: 64%

“…By comparisons of NSP4 sequences, Zhang et al (1998) suggested that changes between amino acids 131 and 140 are important for viral pathogenesis, and demonstrated that a change from amino acid valine to alanine in the NSP4 protein at this position was important in OSU attenuated strains, as it was associated with loss of the ability of inducing diarrhea in mice, which was also observed in a piglet model with virulent and tissue cultureattenuated human RVs Wa strains (WARD et al, 1996). On the other hand, Kirkwood et al (1996) found isoleucine at position 135 in symptomatic children, as well as did Mascarenhas et al (2007).…”

Section: Discussionmentioning

confidence: 88%

“…Tyrosine residue at position 131 of NSP4 coding gene has been postulated to be critical for the diarrheagenic activity of the toxic peptide (BALL et al 1996), but histidine was also found in diarrheic young children (CUNLIFFE et al, 1997;MASCARENHAS et al, 2007). Sequence analysis from porcine strains revealed amino acids serine, alanine and histidine at residue 131 (CIARLET et al, 2000;STEYER et al, 2007;MATTHIJNSSENS et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

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Molecular Analysis of Nsp4 Coding Gene of Porcine Rotavirus in Brazil

Cunha

Brandão²,

Nishida

et al. 2015

Ars Vet

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RESUMOA proteína não estrutural 4 (NSP4) desempenha diferentes funções na replicação e na morfogênese dos rotavírus, apresentando, ainda, uma atividade de enterotoxina, causando diarreia do tipo secretória. Um total de 11 sequências parciais de nucleotídeos do gene codificador da NSP4 de rotavírus suínos de criações brasileiras foram definidas como pertencentes ao grupo A. Comparando-se as sequências virais da área do peptídeo toxigênico, que compreende a porção entre os aminoácidos de 114 a 135, constatou-se uma única mutação pontual no aminoácido 135, sendo que duas amostras apresentaram alanina, e as demais, valina. A análise filogenética do gene demonstrou que todas as amostras pertencem ao genotipo E1, e que a identidade nucleotídica das amostras brasileiras variou de 92,4% a 100%, enquanto que a identidade de aminoácidos, de 95,8% a 100%. Apenas um resíduo (aa 138) sofreu seleção positiva enquanto que pelo menos outros 119 apresentam seleção negativa. Assim, esses dados mostram a ocorrência de um genotipo comum da NSP4 já descrito anteriormente em suínos, com uma baixa diversidade entre as amostras encontradas. PALAVRAS-CHAVE:Genotipos. Proteína não-estrutural. Reoviridae. SUMMARYThe non-structural protein 4 (NSP4) has different roles in rotaviral replication, morphogenesis, and enterotoxin-like activity causing secretory diarrhea. A total of 11 partial nucleotide sequences of NSP4 coding gene were defined from group A rotavirus circulating in Brazilian swine herds. On comparing the viral sequences of diarrheagenic peptide area (amino acid 114-135), there was a single point mutation at amino acid 135 presented by two strains with amino acid alanine, and valine in the others. The NSP4 gene phylogeny showed that all strains clustered into E1 genotype, and the nucleotide identity between Brazilian strains ranged from 92.4% and 100%, while the putative amino acid identity, between 95.8% and 100%. Only one site (138aa) was positively selected and at least 119 were negatively selected. As a conclusion, these data demonstrate the occurrence of a common NSP4 genotype described elsewhere in pigs and low diversity between the samples from the surveyed areas.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

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Molecular Analysis of Nsp4 Coding Gene of Porcine Rotavirus in Brazil

Cunha

Brandão²,

Nishida

et al. 2015

Ars Vet

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“…Evidence exists to show that zoonotic transmission and reassortment of animal rotaviruses occurs. Zoonotic rotavirus strains are capable of causing not only asymptomatic infection but also mild to severe diarrhoea in humans (Gentsch et al, 2005;Ghosh et al, 2007;Khamrin et al, 2006;Mascarenhas et al, 2007;Matthijnssens et al, 2006b;Palombo, 2002;Varghese et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

Steyer

Poljšak-Prijatelj

Barlič–Maganja

et al. 2008

Journal of General Virology

114

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A surveillance of human, porcine and bovine rotaviruses was carried out in Slovenia in 2004 and 2005. Stool samples were collected from a total of 406 pigs (373 from asymptomatic animals), 132 cattle (126 from asymptomatic animals) and 241 humans (all with diarrhoea), tested for group A rotaviruses using RT-PCR and analysed by sequencing. The aims of the study were to determine the incidence of asymptomatic rotavirus infection in animals, to look for evidence of zoonotic transmission and to detect reassortment among rotaviruses. The rates of asymptomatic shedding of rotaviruses in pigs and cattle were 18.0 % (67/373) and 4.0 % (5/126), respectively. Evidence for zoonotic transmission was detected in one human rotavirus strain, SI-MB6, with the G3P[6] genotype combination, as the nucleotide and predicted amino acid sequences of the VP6, VP7, VP8* and NSP4 genes of strain SI-MB6 and of porcine strains showed high nucleotide and amino acid sequence identity. Two porcine rotavirus strains carried VP7 of probable human origin, suggesting an interspecies reassortment event in the past.

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“…Later, genotype P[6] strains were detected sporadically in older children with gastroenteritis worldwide, and investigations revealed that P[6] strains are epidemiologically important in parts of Africa (Bányai et al, 2012). Whilst the majority of virulent human P[6] strains clustered into a single genetic lineage together with strains isolated from neonates, subsequent studies from Japan and Hungary identified genetically highly divergent P[6] strains in children with gastroenteritis (Nakagomi et al, 1999;Bányai et al, 2004), and P[6] genotype diversity in diarrhoeic children has been observed over time (Ahmed et al, 2007;Mascarenhas et al, 2007;Nguyen et al, 2007;Li et al, 2008;Martella et al, 2008;Bányai et al, 2009a, b;Mukherjee et al, 2009Mukherjee et al, , 2011Stupka et al, 2009;Wang et al, 2010). The P[6] VP4 gene can be categorized into at least five lineages, some of which are unique to either pigs or humans, whilst others are shared between humans and swine (Bányai et al, 2004;Martella et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Identification of porcine rotavirus-like genotype P[6] strains in Taiwanese children

Hwang

Bányai

et al. 2012

Journal of Medical Microbiology

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The molecular characterization of genotype P[6] rotavirus strains collected from children admitted to hospital with acute dehydrating diarrhoea during a 6-year surveillance period in Taiwan is described in this study. In total, three G4P[6] strains, one G5P[6] and one G12P [6] were characterized by sequencing and phylogenetic analysis of the VP4, VP7, VP6 and NSP4 genes. Whilst all four genes of the single Taiwanese G12P[6] strain clustered with the respective genes of globally common human rotavirus strains, the G4 and G5 strains showed remarkable similarities to porcine rotavirus strains and putative porcine-origin human P[19] strains reported previously from Taiwan. The overall proportion of porcine rotavirus-like strains in Taiwan remains around 1 % among hospitalized children; however, the circulation and sporadic transmission of these heterotypic strains from pigs to humans could pose a public-health concern. Therefore, continuation of strain monitoring is needed in the vaccine era to detect any possible vaccine breakthrough events associated with the introduction of such heterologous rotavirus strains.

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Detection of a neonatal human rotavirus strain with VP4 and NSP4 genes of porcine origin

Cited by 35 publications

References 59 publications

Molecular Analysis of Nsp4 Coding Gene of Porcine Rotavirus in Brazil

Molecular Analysis of Nsp4 Coding Gene of Porcine Rotavirus in Brazil

Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

Identification of porcine rotavirus-like genotype P[6] strains in Taiwanese children

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