2004
DOI: 10.1111/j.1349-7006.2004.tb03321.x
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Detection of a mouse OGG1 fragment during caspase‐dependent apoptosis: Oxidative DNA damage and apoptosis

Abstract: We investigated the expression of mouse 8-oxoguanine DNA glycosylase 1 (mOGG1) in mouse non-parenchymal hepatocytes (NCTC) during etoposide-or mitomycin C (MMC)-induced apoptosis. We observed mOGG1 fragmentation in apoptotic cells. The apoptosis accompanying the fragmentation of mOGG1 was caspase-dependent. The mOGG1 fragment existed in both the cytoplasm and nucleus of the etoposide-treated NCTC, indicating that the mOGG1 fragment could be transferred into the nucleus. In addition, 8-hydroxyguanine (8-OH-Gua,… Show more

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Cited by 21 publications
(12 citation statements)
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“…In the present study, the fragmentation of mOGG1 was detected in NCTC treated with arsenic compounds. Similar results were obtained in our previous studies, in which we detected the fragmentation of mOGG1 in carcinogentreated mouse liver or during caspase-dependent apoptosis in cultured mouse nonparenchymal hepatocytes (19,25). In those cases and the present study, the 8-OH-Gua accumulation was increased, suggesting that the fragmentation of OGG1 resulted in the increased level of 8-OH-Gua accumulation.…”
Section: Discussionsupporting
confidence: 80%
“…In the present study, the fragmentation of mOGG1 was detected in NCTC treated with arsenic compounds. Similar results were obtained in our previous studies, in which we detected the fragmentation of mOGG1 in carcinogentreated mouse liver or during caspase-dependent apoptosis in cultured mouse nonparenchymal hepatocytes (19,25). In those cases and the present study, the 8-OH-Gua accumulation was increased, suggesting that the fragmentation of OGG1 resulted in the increased level of 8-OH-Gua accumulation.…”
Section: Discussionsupporting
confidence: 80%
“…In addition, caspases are also involved in the generation of ROS (32,33). In fact, z-VAD-fmk prevented VP-16-induced oxidative DNA lesions (23). Activation of caspases could therefore serve to generate the ROS that lead to apoptotic Top1cc.…”
Section: Resultsmentioning
confidence: 99%
“…Because Top1cc can be induced by ROS (22) and oxidative DNA lesions (4, 8 -10) and because VP-16 and doxorubicin generate ROS and oxidative DNA lesions (23,24), we tested whether the apoptotic Top1cc induced by Top2 inhibitors resulted from oxidative lesions. Quenching ROS with the antioxidant N-acetyl-L-cysteine prevented VP-16-induced Top1cc and apoptosis (Fig.…”
Section: Vp-16-induced Top1cc Are Linked To the Generation Of Oxygen mentioning
confidence: 99%
“…In addition, OGG1 appears to be cleaved by caspases during drug-induced apoptosis. 55 Whether OGG1 cleavage merely occurs to remove an antiapoptotic protein or whether it amplifies the death response by creating a pro-apoptotic fragment as it was reported for the cleavage of the MMR protein MLH1 (see mismatch repair section) is presently unknown.…”
Section: P53-binding Proteinsmentioning
confidence: 99%