Detection of a Low Level and Heterogeneous B Cell Immune Response in Peripheral Blood of Acute Borreliosis Patients With High Throughput Sequencing

Kirpach, Josiane; Colone, Alessia; Bürckert, Jean-Philippe; Faison, William J.; Dubois, Anna; Sinner, Regina; Reye, Anna L.

doi:10.3389/fimmu.2019.01105

Cited by 6 publications

(9 citation statements)

References 93 publications

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“…A recent study on circulating plasmablast responses in Lyme disease has provided parallel evidence that selection for Bb specificity can be detected at the repertoire level although the exact VH genes differentially used by these B cells was not reported (5). In this regard, a European study examining the B cell repertoire in peripheral blood of patients with EM found overlap between between IgM and IgG CDR3 sequences during acute and convalescent infection samples in comparison to healthy controls, suggesting that B cell immune responses associated with Bb infection may be detectable in the circulating repertoire (35).…”

Section: Discussionmentioning

confidence: 99%

“…In this regard, a European study examining the B cell repertoire in peripheral blood of patients with EM found an overlap between IgM and IgG CDR3 sequences during acute and convalescent infection samples compared with healthy controls, suggesting that B cell immune responses associated with B . burgdorferi infection may be detectable in the circulating repertoire ( 35 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Single-cell immunophenotyping of the skin lesion erythema migrans identifies IgM memory B cells

Jiang¹,

Meng²,

Raddassi³

et al. 2021

JCI Insight

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The skin lesion erythema migrans (EM) is an initial sign of the Ixodes-tick transmitted Borreliella spirochetal infection known as Lyme disease. T cells and innate immune cells have previously been shown to predominate the EM lesion and promote the reaction. Despite the established importance of B cells and antibodies in preventing infection, the role of B cells in the skin immune response to Borreliella is unknown. Here, we used single-cell RNA-Seq in conjunction with B cell receptor (BCR) sequencing to immunophenotype EM lesions and their associated B cells and BCR repertoires. We found that B cells were more abundant in EM in comparison to autologous uninvolved skin; many were clonally expanded and had circulating relatives. EM-associated B cells upregulated expression of MHC class II genes and exhibited preferential IgM isotype usage. A subset also exhibited low levels of somatic hypermutation despite a gene expression profile consistent with memory B cells. Our study demonstrates that single-cell gene expression with paired BCR sequencing can be used to interrogate the sparse B cell populations in human skin, and reveals that B cells in the skin infection site in early Lyme disease express a phenotype consistent with local antigen presentation and antibody production.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Single-cell immunophenotyping of the skin lesion erythema migrans identifies IgM memory B cells

Jiang¹,

Meng²,

Raddassi³

et al. 2021

JCI Insight

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“…Shared clones from selected cohorts of patients can also be used as multiparametric biomarkers for diagnosis and disease monitoring [ 99 , 100 ].…”

Section: Tcr and Infectious Diseasesmentioning

confidence: 99%

T-Cell Receptor Repertoire Sequencing and Its Applications: Focus on Infectious Diseases and Cancer

Mazzotti

Gaimari

Bravaccini

et al. 2022

IJMS

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The immune system is a dynamic feature of each individual and a footprint of our unique internal and external exposures. Indeed, the type and level of exposure to physical and biological agents shape the development and behavior of this complex and diffuse system. Many pathological conditions depend on how our immune system responds or does not respond to a pathogen or a disease or on how the regulation of immunity is altered by the disease itself. T-cells are important players in adaptive immunity and, together with B-cells, define specificity and monitor the internal and external signals that our organism perceives through its specific receptors, TCRs and BCRs, respectively. Today, high-throughput sequencing (HTS) applied to the TCR repertoire has opened a window of opportunity to disclose T-cell repertoire development and behavior down to the clonal level. Although TCR repertoire sequencing is easily accessible today, it is important to deeply understand the available technologies for choosing the best fit for the specific experimental needs and questions. Here, we provide an updated overview of TCR repertoire sequencing strategies, providers and applications to infectious diseases and cancer to guide researchers’ choice through the multitude of available options. The possibility of extending the TCR repertoire to HLA characterization will be of pivotal importance in the near future to understand how specific HLA genes shape T-cell responses in different pathological contexts and will add a level of comprehension that was unthinkable just a few years ago.

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“…Notably, the public antibody clones have been confirmed to exist on multiple HIV-infected individuals by analysis of the antibody repertoire 128 . It has been reported that the shared clones or overlapped sequences derived from diverse patients can be served as biomarkers for diagnosis 129 , 130 . The detailed characterization of antibody repertoire may be beneficial to vaccine evaluation.…”

Section: Advances Of Ir-seq In Clinical Applicationmentioning

confidence: 99%

The methods and advances of adaptive immune receptors repertoire sequencing

Pan

Tang

et al. 2021

Theranostics

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The adaptive immune response is a powerful tool, capable of recognizing, binding to, and neutralizing a vast number of internal and external threats via T or B lymphatic receptors with widespread sets of antigen specificities. The emergence of high-throughput sequencing technology and bioinformatics provides opportunities for research in the fields of life sciences and medicine. The analysis and annotation for immune repertoire data can reveal biologically meaningful information, including immune prediction, target antigens, and effective evaluation. Continuous improvements of the immunological repertoire sequencing methods and analysis tools will help to minimize the experimental and calculation errors and realize the immunological information to meet the clinical requirements. That said, the clinical application of adaptive immune repertoire sequencing requires appropriate experimental methods and standard analytical tools. At the population cell level, we can acquire the overview of cell groups, but the information about a single cell is not obtained accurately. The information that is ignored may be crucial for understanding the heterogeneity of each cell, gene expression and drug response. The combination of high-throughput sequencing and single-cell technology allows us to obtain single-cell information with low-cost and high-throughput. In this review, we summarized the current methods and progress in this area.

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Detection of a Low Level and Heterogeneous B Cell Immune Response in Peripheral Blood of Acute Borreliosis Patients With High Throughput Sequencing

Cited by 6 publications

References 93 publications

Single-cell immunophenotyping of the skin lesion erythema migrans identifies IgM memory B cells

Single-cell immunophenotyping of the skin lesion erythema migrans identifies IgM memory B cells

T-Cell Receptor Repertoire Sequencing and Its Applications: Focus on Infectious Diseases and Cancer

The methods and advances of adaptive immune receptors repertoire sequencing

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