Detection of a Gene in Pea Controlling Nonhost Resistance to<i>Pseudomonas syringae</i>pv.<i>phaseolicola</i>

Wood, John R.; Vivian, Alan; Jenner, Carol E.; Mansfıeld, John W.; Taylor, John D.

doi:10.1094/mpmi-7-0534

Cited by 20 publications

(17 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using exonuclease 111 deletion clones in an in uiuo replication assay, the minimal fragment from pAKC able to support replication in P. syringae (oriV-pPT23A) was defined as a 1.6 kb fragment. (Wood et al, 1994), was found by hybridization to be similar to oriV-pPT23A (data not shown). Plasmids pAV514 and pAV51.5, which contain the 4-3 kb PstI fragment from pPPY4O in pK184 in different orientations, replicated autonomously in strain PT23 and were compatible with plasmid pPT23A, even after four transfers in media with kanamycin.…”

Section: Resultsmentioning

confidence: 87%

“…Since rulAB genes are present in many pPT23A-like plasmids Sesma et al, 1998), it is possible that genes for UV resistance could contribute to the fitness of this plasmid family, and perhaps favour the accumulation of several of its members in a given cell. Wood et al (1994) described a gene library cosmid, pPPY40, and showed that an avirulence phenotype, which matched a resistance gene in pea, required two regions, I and 11, as shown by subcloning and transposon mutagenesis. Subsequently, it was shown that cosmids with Tn3Gus insertions in region I1 were very unstable in P. syringae pv.…”

Section: Discussionmentioning

confidence: 99%

“…incompatibility assays. The identification of determinants in pAKC showing incompatibility against the parent plasmid pPT23A was assayed by a qualitative test essentially as Jobling & Holmes (1990) Wood et al (1994) This work…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, an avirulence phenotype, which matched a resistance gene in pea (Pisum sativum), was shown to require the presence of two cloned regions of DNA, designated regions I and 11. The two regions were separated by approximately 4 kb and located on pAV50.5 (Wood et al, 1994). The cloned region I1 displayed a strong incompatibility with pAV505 (Jackson, 1997) and hybridized with the minimal replication region of pPT23A.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Replication regions from plant-pathogenic Pseudomonas syringae plasmids are similar to ColE2-related replicons

et al. 1999

Self Cite

View full text Add to dashboard Cite

Many strains of the phytopathogen Pseudomonas syringae contain mutually compatible plasmids that share extensive regions of sequence homology and essential replication determinants. The replication regions of two compatible large plasmids involved in virulence or pathogenicity, pPT23A from P. syringae pv. tomato strain PT23 and pAV505 from P. syringae pv. phaseolicola strain HR11302A, were isolated. DNA sequencing of the origins of replication revealed homologous ORFs, designated ORF-Pto and ORF-Pph, respectively. Both ORFs are 1311 bp long and encode peptides of 437 amino acids with predicted molecular masses of 48259 (Pto) and 48334 (Pph) Da. Expression of the two ORFs in Escherichia coli produced peptides of 50 kDa (Pto) and 56 kDa (Pph). The predicted peptides showed an overall identity of 897 %, being highly conserved from residues 1 to 373, but showing considerable variation in their C-terminal regions (50% identity over the last 64 aa). The two ORFs had significant similarity with the putative replication protein from plasmid pTiKl2 of Thiobacillus intermedius and other ColE2-related plasmids. However, both peptides were 100 residues longer than any of the known ColE2-related rep sequences. Subcloning of fragments from the replication region of pPT23A revealed the presence of a t least three incompatibility determinants, designated IncA, lncB and IncC. Partial sequencing of the region downstream of ORF-Pto revealed homology to the rulAB genes, involved in UV resistance, from plasmid pPSR1. It is proposed that the replication origin of pPT23A serves as the prototype of a family of related plasmids.

show abstract

Section: Resultsmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Replication regions from plant-pathogenic Pseudomonas syringae plasmids are similar to ColE2-related replicons

et al. 1999

Self Cite

View full text Add to dashboard Cite

show abstract

“…The HR is a resistance response recognized by the rapid death of plant cells at inoculation sites and the restriction of microbial colonization (2). Additional avr genes located on the plasmid in Pph determine ability to elicit the HR in nonhost plants, avrPphC and a homolog of avrD (soybean interactors), and avrPphD that interacts with pea (6)(7)(8).…”

mentioning

confidence: 99%

Identification of a pathogenicity island, which contains genes for virulence and avirulence, on a large native plasmid in the bean pathogenPseudomonas syringaepathovar phaseolicola

Jackson

Athanassopoulos

Tsiamis

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

300

253

View full text Add to dashboard Cite

The 154-kb plasmid was cured from race 7 strain 1449B of the phytopathogen Pseudomonas syringae pv. phaseolicola (Pph). Cured strains lost virulence toward bean, causing the hypersensitive reaction in previously susceptible cultivars. Restoration of virulence was achieved by complementation with cosmid clones spanning a 30-kb region of the plasmid that contained previously identified avirulence (avr) genes avrD, avrPphC, and avrPphF. Single transposon insertions at multiple sites (including one located in avrPphF) abolished restoration of virulence by genomic clones. Sequencing 11 kb of the complementing region identified three potential virulence (vir) genes that were predicted to encode hydrophilic proteins and shared the hrp-box promoter motif indicating regulation by HrpL. One gene achieved partial restoration of virulence when cloned on its own and therefore was designated virPphA as the first (A) gene from Pph to be identified for virulence function. In soybean, virPphA acted as an avr gene controlling expression of a rapid cultivar-specific hypersensitive reaction. Sequencing also revealed the presence of homologs of the insertion sequence IS100 from Yersinia and transposase Tn501 from P. aeruginosa. The proximity of several avr and vir genes together with mobile elements, as well as G؉C content significantly lower than that expected for P. syringae, indicates that we have located a plasmidborne pathogenicity island equivalent to those found in mammalian pathogens.Varietal resistance to halo-blight disease of bean (Phaseolus vulgaris L.) caused by Pseudomonas syringae pv. phaseolicola (Pph) is determined by gene-for-gene interactions involving five resistance (R) genes in the host and five matching avirulence (avr) genes in the pathogen. Depending on the presence or absence of functional avr genes, nine races of Pph have been distinguished (1, 2). The avr genes matching R1, R2, and R3 have been cloned and sequenced. Their full designations are avrPphF.R1, avrPphE.R2, and avrPphB.R3; the terminal R gene designation will not be used here (3-5). Both avrPphE and avrPphB are chromosomal, whereas avrPphF is located on a large plasmid in those races that cause the hypersensitive reaction (HR) in cultivars of bean with the matching R1 gene.

show abstract

Avirulence geneavrPpiAfromPseudomonas syringaepv.pisiis not required for full virulence on pea

Gibbon

Jenner

Mur

et al. 1997

Physiological and Molecular Plant Pathology

View full text Add to dashboard Cite

Detection of a Gene in Pea Controlling Nonhost Resistance toPseudomonas syringaepv.phaseolicola

Cited by 20 publications

References 0 publications

Replication regions from plant-pathogenic Pseudomonas syringae plasmids are similar to ColE2-related replicons

Replication regions from plant-pathogenic Pseudomonas syringae plasmids are similar to ColE2-related replicons

Identification of a pathogenicity island, which contains genes for virulence and avirulence, on a large native plasmid in the bean pathogenPseudomonas syringaepathovar phaseolicola

Avirulence geneavrPpiAfromPseudomonas syringaepv.pisiis not required for full virulence on pea

Contact Info

Product

Resources

About