Detection of a distinct gill-surface antibody response following horizontal infection and bath challenge of brook trout Salvelinus fontinalis with Flavobacterium branchiophilum. the causative agent of bacterial gill disease

Lumsden, John S.; Ostland, V. E.; Byrne, Philip; Ferguson, H. W.

doi:10.3354/dao016021

Cited by 50 publications

(34 citation statements)

References 13 publications

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“…This indicates the importance of the gills in immersion vaccination. Specific antibodies have been detected in the gills of brook trout and rainbow trout following 70 immersion infection or immunisation with Flavobacterium branchiophilium, respectively (Lumsden et al, 1993(Lumsden et al, , 1995. These authors showed that immersion vaccinated rainbow trout were better protected against experimental bath challenge than i.p.…”

Section: Discussionmentioning

confidence: 62%

The gill is a major organ for antibody secreting cell production following direct immersion of sea bass (Dicentrarchus labrax, L.) in a Photobacterium damselae ssp. piscicida bacterin: an ontogenetic study

Santos

Taverne-Thiele

Barnes

et al. 2001

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 62%

The gill is a major organ for antibody secreting cell production following direct immersion of sea bass (Dicentrarchus labrax, L.) in a Photobacterium damselae ssp. piscicida bacterin: an ontogenetic study

Santos

Taverne-Thiele

Barnes

et al. 2001

Fish & Shellfish Immunology

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“…The development of mucosal antibody with strong memory may require multiple exposures at the proper site (Ganguly & Waldman 1983). In a more analogous situation, Lumsden et al (1993) found that there was little correlation between levels of gill surface and cutaneous mucus antibody in brook trout Salvelinus fontinalis following infection with Flavobacterium branchiophilum, the etiologic agent of bacterial gill disease. Failure to detect antibody in mucus is consistent with Smith (1990), who found anti-dinospore activity in skin mucus of only 25% of tilapia immunized with dinospores.…”

Section: Discussionmentioning

confidence: 93%

“…The existence of a localized antibody response in fish has been widely proposed and studies of skin, intestine and bile (Ourth 1980, Lobb & Clem 1981a, b, St. LouisCormier et al 1984, Georgopoulou & Vernier 1986, Rombout et al 1986, Lobb 1987, Lumsden et al 1993) have supported such a possibility. ELISA results using skin mucus from tomato clownfish were almost completely negative with only one fish showing positive results.…”

Section: Discussionmentioning

confidence: 95%

Acquired immunity to amyloodiniosis is associated with an antibody response

Cobb¹,

Levy²,

Noga³

1998

Dis. Aquat. Org.

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The dinoflagellate Amyloodlnium ocellatum, which causes amyloodiniosis or 'marine velvet disease', 1s one of the most senous ectoparasitic diseases plaguing warmwater manne fish culture worldwide We report that tomato clownfish Amphlpnon frenatus develop strong immunity to Amyloodlnlum ocellatum infection following repeated nonlethal challenges and that specific ant~bodies are associated with this response React~on of immune fish antisera against dinospore and trophont-denved antigens in Western blots indicated both shared and stage-speclfic antibody-antigen reactions A mannan-binding-protein affinity column was used to isolate IgM-like antibody from A frenatus serum The reduced Ig consisted of one 70 kD heavy chain and one 32 kD light chain with an estimated molecular weight of 816 kD for the native molecule Immunoglobulin (Ig) isolated from immune but not nonunmune fish serum significantly inhlblted parasite infectivity In vitro An enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal rabbit antibody produced a g a~n s t aff~nity-punfied A frenatus Ig Anti-Amyloodlnium serum antibody was not always detectable in immune fish although serum antibody titers in immune fish increased after repeated exposure to the parasite These results suggest that there may be a localized antibody response in skin/gill epithelia1 tissue although antibody was rarely detected in skin mucus

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“…A flow diagram of Trial 2 is shown in Table l . Trial 3 -detection of local antibody from mucus using ELISA. Gills of 10 Atlantic salmon (200 & 14.2 g), experimentally infected with AGD by cohabitation for 6 to 8 wk were perfused and the mucus was extracted according to the method described by Lumsden et al (1993). Mucus was extracted in the same way from 6 naive fish.…”

Section: Methodsmentioning

confidence: 99%