Detection of a Cell Proliferation-Inhibiting Factor in the Cell-Free Ascitic Fluid of Intraperitoneal Mouse Tumors

Olivares, Enrique G.; Rodriguez-Vedia, N.

doi:10.1159/000226000

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…Association between immunosuppression, anaemia, thrombocytopenia and advanced cancer have been described in both mouse and human [I, 2]. A great variety of soluble human and murine tumour-derived factors which suppress the immune functions in vitro have been found in tumour culture supernatants and in sera or ascitic fluids from tumourbearing animals and humans [3][4][5][6][7][8][9][10]. Such factors cover a wide molecular weight range and have been shown to inhibit a variety of lymphocyte functions including inhibition of J Member of the Research Career, CONICET (Consejo Nacional de Investigaciones Cientificas y Tecnicas).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Activity of Soluble IL‐2R in Sera, Ascites and Culture Supernatants from Murine Leukaemic Cells

et al. 1994

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The effect of sera from mice bearing a T cell lymphoid leukaemia (LB) and the supernatants from short term cultures of the tumour cells were studied on cell proliferation using syngeneic and allogeneic normal and tumour cells. An inhibitory activity was demonstrated in 24-48 h supernatants of LB cells in culture and disappeared after 4 days of culture. Inhibitory activity was cytostatic but not cytotoxic and was non-specific since it inhibited the growth of both syngeneic and allogeneic normal and tumour cells. Such activity was found in the 10(5)-1.3 x 10(5) M(r) serum fraction after a Sephacryl S200 chromatography. Though sensitive to protease, trypsin or neuraminidase treatment, which indicated its glycoprotein nature, it remained stable after heating or freezing-thawing cycles as well as after alkaline, acid or hyaluronidase treatment. Addition of exogenous IL-2 abrogated inhibitory activity. ELISA showed the presence of soluble IL-2R both in LB conditioned medium and in above serum fraction. It is demonstrated that the inhibitory factor, soluble IL-2R, is produced by LB leukaemia cells, then secreted into blood and ascitic fluid or released into culture supernatants. Soluble IL-2R exerts inhibitory activity blocking cell proliferation and modulating immune response by binding to free IL-2.

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Section: Introductionmentioning

confidence: 99%

Inhibitory Activity of Soluble IL‐2R in Sera, Ascites and Culture Supernatants from Murine Leukaemic Cells

et al. 1994

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Detection of Immunoregulatory Lipid‐Like Factors in Human Amniotic Fluid

Rueda

Vargas

García-Pacheco

et al. 1990

American J Rep Immunol

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The immunosuppressive activity of amniotic fluid (AF) is extensively documented in the mouse. Although this property is due in part to the presence of alpha-fetoprotein (alpha-FP), other immunosuppressive factors are suspected. In this article, we demonstrate that human amniotic fluid lipid extract (AFLE) is inhibitory of, although not cytotoxic to, PHA-activated human lymphocytes, of mouse bone marrow cells, and of different established cell lines of human and mouse source. This effect is shown to be reversible. Under preparative thin layer chromatography (TLC) using chloroform:methanol:water (60:38:8) as solvents, the activity of AFLE migrates to two peaks of inhibition with Rf values of 0.46-0.62 and 0.84-1, respectively. These lipid-like factors may play a role as a nonspecific immunoregulatory mechanism which prevents maternally mediated immune rejection of the conceptus.

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Detection of a Cell Proliferation-Inhibiting Factor in the Cell-Free Ascitic Fluid of Intraperitoneal Mouse Tumors

Cited by 2 publications

References 9 publications

Inhibitory Activity of Soluble IL‐2R in Sera, Ascites and Culture Supernatants from Murine Leukaemic Cells

Inhibitory Activity of Soluble IL‐2R in Sera, Ascites and Culture Supernatants from Murine Leukaemic Cells

Detection of Immunoregulatory Lipid‐Like Factors in Human Amniotic Fluid

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