Detection of a carcinoembryonic antigen using aptamer-modified film bulk acoustic resonators

Zheng, Dewen; Xiong, Juan; Guo, Peng; YueBin, Li; Wang, Shengfu; Gu, Haoshuang

doi:10.1016/j.materresbull.2014.07.054

Cited by 12 publications

(7 citation statements)

References 20 publications

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“…The major difference occurs in the area of functionalizing the FBAR with bioreceptors, for the selective detection of the target analyte from the sample. The bioreceptors used are similar to any of the acoustic biosensors, such as an antibody, aptamers, and nucleic acid probes [178][179][180]. The backside air cavity design enables the bioreceptors to be functionalized on the backside cavity, after coating the cavity area beneath the membrane with an Au sensing layer [181].…”

Section: Fbar As Biosensors: Operating Principlementioning

confidence: 99%

Acoustic Biosensors and Microfluidic Devices in the Decennium: Principles and Applications

Nair

Teo

2021

Micromachines

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Lab-on-a-chip (LOC) technology has gained primary attention in the past decade, where label-free biosensors and microfluidic actuation platforms are integrated to realize such LOC devices. Among the multitude of technologies that enables the successful integration of these two features, the piezoelectric acoustic wave method is best suited for handling biological samples due to biocompatibility, label-free and non-invasive properties. In this review paper, we present a study on the use of acoustic waves generated by piezoelectric materials in the area of label-free biosensors and microfluidic actuation towards the realization of LOC and POC devices. The categorization of acoustic wave technology into the bulk acoustic wave and surface acoustic wave has been considered with the inclusion of biological sample sensing and manipulation applications. This paper presents an approach with a comprehensive study on the fundamental operating principles of acoustic waves in biosensing and microfluidic actuation, acoustic wave modes suitable for sensing and actuation, piezoelectric materials used for acoustic wave generation, fabrication methods, and challenges in the use of acoustic wave modes in biosensing. Recent developments in the past decade, in various sensing potentialities of acoustic waves in a myriad of applications, including sensing of proteins, disease biomarkers, DNA, pathogenic microorganisms, acoustofluidic manipulation, and the sorting of biological samples such as cells, have been given primary focus. An insight into the future perspectives of real-time, label-free, and portable LOC devices utilizing acoustic waves is also presented. The developments in the field of thin-film piezoelectric materials, with the possibility of integrating sensing and actuation on a single platform utilizing the reversible property of smart piezoelectric materials, provide a step forward in the realization of monolithic integrated LOC and POC devices. Finally, the present paper highlights the key benefits and challenges in terms of commercialization, in the field of acoustic wave-based biosensors and actuation platforms.

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Section: Fbar As Biosensors: Operating Principlementioning

confidence: 99%

Acoustic Biosensors and Microfluidic Devices in the Decennium: Principles and Applications

Nair

Teo

2021

Micromachines

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“…The response time of the bioreceptor was also found to be good and comparable or better than some of the reported values by various researchers. [26][27][28][29] In the present study, the equilibrium plateau was attained for each immunoassay run within a minimum time period of 15-30 minutes. The limit of detection for both the biosensors was calculated as blank frequency response by PBS + 3Â noise (standard deviation).…”

Section: Immunoassay Run For Cea Solutionsmentioning

confidence: 99%

Graphene oxide-Au nano particle coated quartz crystal microbalance biosensor for the real time analysis of carcinoembryonic antigen

et al. 2020

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“…The binding ratio for protein G is 10% higher than that of protein A (20.8%), indicating that protein G can immobilize more antibody than protein A and/or provide better antibody orientations. More recently, Zheng et al (Zheng et al 2014;Zheng et al 2016b) have also used AlN-based FBARs to detect the CEA.…”

Section: Fbars As Protein Biosensorsmentioning

confidence: 99%

“…(B) fr shifts of FBAR after the adsorption of anti-hPSA antibody (5 mg/L) for 15 min, BSA (50 mg/L) blocking for 15 min and antigen (hPSA, 5 mg/L) binding for 15 min (Zhao et al 2014). (Copyright © 2014 Elsevier Ltd.)Other than PSA, FBARs have also been fabricated to detect other cancer biomarkers such as epithelial tumour marker (MUC1)Zheng et al 2016a), Alpha-fetoprotein (AFP)) and carcinoembryonic antigen (CEA)Lee and Song 2010;Zheng et al 2014;Zheng et al 2016b). An AlN-based FBAR was fabricated with fr approximately 575 MHz as an immunosensor to detect MUC1.…”

mentioning

confidence: 99%

Film bulk acoustic resonators (FBARs) as biosensors: A review

Zhang

Luo

Flewitt

et al. 2018

Biosensors and Bioelectronics

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Biosensors play important roles in different applications such as medical diagnostics, environmental monitoring, food safety, and the study of biomolecular interactions. Highly sensitive, label-free and disposable biosensors are particularly desired for many clinical applications. In the past decade, film bulk acoustic resonators (FBARs) have been developed as biosensors because of their high resonant frequency and small base mass (hence greater sensitivity), lower cost, label-free capability and small size. This paper reviews the piezoelectric materials used for FBARs, the optimisation of device structures, and their applications as biosensors in a wide range of biological applications such as the detection of antigens, DNAs and small biomolecules. Their integration with microfluidic devices and high-throughput detection are also discussed.

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Detection of a carcinoembryonic antigen using aptamer-modified film bulk acoustic resonators

Cited by 12 publications

References 20 publications

Acoustic Biosensors and Microfluidic Devices in the Decennium: Principles and Applications

Acoustic Biosensors and Microfluidic Devices in the Decennium: Principles and Applications

Graphene oxide-Au nano particle coated quartz crystal microbalance biosensor for the real time analysis of carcinoembryonic antigen

Film bulk acoustic resonators (FBARs) as biosensors: A review

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