Detection of 14 High-risk Human Papillomavirus (HPV) Genotypes Within the Italian Cervical Cancer Screening

The Rare Condition of Double Cervix: Results from the High-Risk Human Papillomavirus-based Cervical Cancer Screening Program in the Lazio Region

“…Our data shows that 16.7% of the women with double cervix resulted as HR-HPV positives; this percentage is slightly higher than the overall population examined (13.9%) [5], in our Laboratory.…”

“…It is known that the main risk factor for development of cervical cancer is HR-HPV infection: therefore, it is preventable and can be easily treated if detected at early stages [6]. To prevent cervical neoplastic pathologies, the Lazio Region, according to the National indications, structured a screening program involving a large number of women [5]. The gynaecological examination followed by cervix-brush performed on a large number of women allowed to identify women with genital malformation such as the double cervix.…”

“…The aim of screening campaigns is to monitor the persistence of HR-HPV in order to early diagnose and treat dysplastic lesions before they evolve into an infiltrating neoplasm [5].…”

“…DNA extraction, PCR amplification and extended genotyping were performed using automated DNA extraction and PCR setup platform STARlet IVD (Seegene, Seoul, Republic of Korea). Anyplex™ II HR-HPV Detection kit (Seegene, Seoul, Republic of Korea) was applied for HPV DNA detection and genotyping using the CFX96™ Real-time PCR System (Bio-Rad, Berkeley, CA, USA), as already reported [5]. Our molecular platform (the Seegene's proprietary DPO™ and TOCE TM technologies), allows the detection and genotyping of the following 14 HR-HPV subtypes 16,18,31,33,35,39,45,51,52,56,58,59.…”

“…Our molecular platform (the Seegene's proprietary DPO™ and TOCE TM technologies), allows the detection and genotyping of the following 14 HR-HPV subtypes 16,18,31,33,35,39,45,51,52,56,58,59. 66 and 68 [5].…”