Detection Methods for Shiga Toxins and Shiga Toxin-Producing E. coli

Silva, Christopher J.; Brandon, David L.; Skinner, Craig; He, Xiaohua

doi:10.1007/978-3-319-50580-0_6

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2018

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“…Shiga toxins are large multicomponent toxins produced by phage-infected bacterial food contaminants. There are a variety of means to detect Shiga toxins to determine the source of contamination . Regulatory agencies focus on the E.…”

Section: Introductionmentioning

confidence: 99%

Food Forensics: Using Mass Spectrometry To Detect Foodborne Protein Contaminants, as Exemplified by Shiga Toxin Variants and Prion Strains

Silva

2018

J. Agric. Food Chem.

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Food forensicists need a variety of tools to detect the many possible food contaminants. As a result of its analytical flexibility, mass spectrometry is one of those tools. Use of the multiple reaction monitoring (MRM) method expands its use to quantitation as well as detection of infectious proteins (prions) and protein toxins, such as Shiga toxins. The sample processing steps inactivate prions and Shiga toxins; the proteins are digested with proteases to yield peptides suitable for MRM-based analysis. Prions are detected by their distinct physicochemical properties and differential covalent modification. Shiga toxin analysis is based on detecting peptides derived from the five identical binding B subunits comprising the toxin. N-labeled internal standards are prepared from cloned proteins. These examples illustrate the power of MRM, in that the same instrument can be used to safely detect and quantitate protein toxins, prions, and small molecules that might contaminate our food.

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Section: Introductionmentioning

confidence: 99%