Detection method for reverse transcription recombinase-aided amplification of avian influenza virus subtypes H5, H7, and H9
Zongshu Zhang,
Zichuang Zhang,
Chunguang Wang
et al.
Abstract:Background
Avian influenza virus (AIV) not only causes huge economic losses to the poultry industry, but also threatens human health. Reverse transcription recombinase-aided amplification (RT-RAA) is a novel isothermal nucleic acid amplification technology. This study aimed to improve the detection efficiency of H5, H7, and H9 subtypes of AIV and detect the disease in time. This study established RT-RAA-LFD and real-time fluorescence RT-RAA (RF-RT-RAA) detection methods, which combined RT-RAA w… Show more
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