Detection limits in photothermal microscopy

Gaiduk, Alexander; Ruijgrok, Paul V.; Yorulmaz, Mustafa; Orrit, Michel

doi:10.1039/c0sc00210k

Cited by 195 publications

(327 citation statements)

References 29 publications

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“…Nevertheless, our sensitivity with tunable pulsed excitation is still comparable to the limits set in the literature, 29 and we are able to not only image an individual 20 nm gold nanoparticle, but also record its absorption spectrum, in very good agreement with Mie theory calculations that include 10 multipolar modes (Fig. 2e).…”

supporting

confidence: 69%

“…29 Changing the excitation power by a factor of 3 furthermore does not result in significant broadening of the plasmon resonance (Fig. S10), as would be expected for an electronic signal.…”

mentioning

confidence: 75%

“…21 Indirect detection schemes combined with modulation techniques have been developed for absorption-based detection, [22][23][24][25] 4 including ground-state depletion microscopy 15,26 and photothermal microscopy. 11,13,14,16,[27][28][29][30] However, most studies using these methods have reported single-wavelength imaging without yielding spectroscopic information. A tunable dye laser, incorporated as the excitation source into a photothermal microscope, has allowed the acquisition of absorption, albeit in a limited spectral range of 515 nm to 585 nm, for which chromatic aberrations were mostly negligible.…”

mentioning

confidence: 99%

“…We furthermore confirm the photothermal nature of the signal to rule out other contributions, such as electronic transitions. 47 Because the photothermal signal is proportional to the change in the medium's refractive index per temperature increase and to the heating power, 13,29 we compare the SNR of the same nanoparticles in different media and perform a power dependence.…”

mentioning

confidence: 99%

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Single-Particle Absorption Spectroscopy by Photothermal Contrast

et al. 2015

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Removing effects of sample heterogeneity through single-molecule and single-particle techniques has advanced many fields. While background free luminescence and scattering spectroscopy is widely used, recording the absorption spectrum only is rather difficult. Here we present an approach capable of recording pure absorption spectra of individual nanostructures. We demonstrate the implementation of single-particle absorption spectroscopy on strongly scattering plasmonic nanoparticles by combining photothermal microscopy with a supercontinuum laser and an innovative calibration procedure that accounts for chromatic aberrations and wavelength-dependent excitation powers. Comparison of the absorption spectra to the scattering spectra of the same individual gold nanoparticles reveals the blueshift of the absorption spectra, as predicted by Mie theory but previously not detectable in extinction measurements that measure the sum of absorption and scattering. By covering a wavelength range of 300 nm, we are furthermore able to record absorption spectra of single gold nanorods with different aspect ratios. We find that the spectral shift between absorption and scattering for the longitudinal plasmon resonance decreases as a function of nanorod aspect ratio, which is in agreement with simulations.

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supporting

confidence: 69%

“…29 Changing the excitation power by a factor of 3 furthermore does not result in significant broadening of the plasmon resonance (Fig. S10), as would be expected for an electronic signal.…”

mentioning

confidence: 75%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Single-Particle Absorption Spectroscopy by Photothermal Contrast

et al. 2015

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“…What is more, intrinsic contrast from absorbing molecules such as hemoglobin or proteins contained in mitochondria has also been examined in living biological samples [10,11]. The sensitivity of this method is such that even the detection of single molecules has been reported [12].…”

Section: Introductionmentioning

confidence: 99%

Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy

Pache¹,

Bocchio²,

Bouwens³

et al. 2012

Opt. Express

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Abstract:We introduce photothermal optical lock-in Optical Coherence Microscopy (poli-OCM), a volumetric imaging technique, which combines the depth sectioning of OCM with the high sensitivity of photothermal microscopy while maintaining the fast acquisition speed inherent to OCM. We report on the detection of single 40 nm gold particles with a 0.5 µm lateral and 2 µm axial resolution over a 50 µm depth of field and the three-dimensional localization of gold colloids within living cells. In combination with intrinsic sample contrast measured with dark-field OCM, poli-OCM offers a versatile platform for functional cell imaging.

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Single‐Molecule Detection and Spectroscopy

Orrit

2015

Photonics

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Detection limits in photothermal microscopy

Cited by 195 publications

References 29 publications

Single-Particle Absorption Spectroscopy by Photothermal Contrast

Single-Particle Absorption Spectroscopy by Photothermal Contrast

Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy

Single‐Molecule Detection and Spectroscopy

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