Detection, identification, and comparison of Capnocytophaga, Bacteroides ochraceus, and DF-1

Newman, Michael G.; Sutter, Vera L.; Pickett, M. J.; Blachman, Uzy; Greenwood, J.; Grinenko, V; Citron, Diane M.

doi:10.1128/jcm.10.4.557-562.1979

Cited by 59 publications

(13 citation statements)

References 11 publications

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“…Capnocytophaga is a fastidious, fusiform, non-spore-forming, gliding, Gram-negative bacillus, usually isolated from the oral cavity and rarely found in the genital tract. Previously known as CDC biogroup DF-1 and Bacteroides ochraceus, this genus was discovered in 1979 and includes three species: Capnocytophaga ochracea, C. sputigena and Capnocytophaga gingivalis [27]. Identification and sequencing of 16S rRNA genes in culture-negative amniotic fluid from women in premature labour may have helped to reveal the bacterial origin of preterm labour, as bacterial organisms such as capnocytophagae can be identified by this molecular approach [28].…”

Section: CMImentioning

confidence: 99%

Capnocytophaga speciesand preterm birth Case Series and Review of the Literature

López

Raymond

Patkaï

et al. 2009

Clinical Microbiology and Infection

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Capnocytophaga, a genus of Gram-negative anaerobes that inhabit the oral cavity, has been reported to be an unusual cause of chorioamnionitis and neonatal infection. We report five cases of Capnocytophaga spp. infections in preterm infants (one proven infection and four probable infections) and review 14 previously reported cases. We suggest that Capnocytophaga sp. may be responsible for some occult causes of chorioamnionitis or preterm birth, and that the prevalence of this infection may be higher than previously reported.

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Section: CMImentioning

confidence: 99%

Capnocytophaga speciesand preterm birth Case Series and Review of the Literature

López

Raymond

Patkaï

et al. 2009

Clinical Microbiology and Infection

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“…The study of the stability of pBY22 in different strains of E. coli K-12 established that this plasmid was stably maintained in E. coli K-12. Guiney and Davis (14) demonstrated that two plasmids from Bacteroides ochraceus were stable in E. coli after conjugation; however, this species of Bacteroides has recently been shown to be synonymous with the genus Capnocytophaga (24,37,41). Although pBY22 in E. coli transformants is a nonconjugative plasmid, it could be mobilized by conjugative plasmid .…”

Section: Resultsmentioning

confidence: 99%

Stability in Escherichia coli of an antibiotic resistance plasmid from Bacteroides fragilis

Rashtchian¹,

Booth²

1981

J Bacteriol

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A Bacteroides fragilis strain resistant to penicillin G, tetracycline, and clindamycin was screened for the presence of plasmid deoxyribonucleic acid (DNA). Agarose gel electrophoresis of ethanol-precipitated DNA from cleared lysates of this strain revealed two plasmid DNA bands. The molecular weights of the plasmids were estimated by their relative mobility in agarose gel and compared with standard plasmids with known molecular weights. The molecular weights were 3.40 ± 0.20 x 106 and 1.95 ± 0.05 x 106 for plasmids pBY1 and pBY2, respectively. Plasmid DNA purified by cesium chloride-ethidium bromide gradient centrifugation was used to transform a restrictionand modification-negative strain of Escherichia coli. Penicillin Gand tetracycline-resistant transformants were screened for the presence of plasmid DNA. A plasmid band corresponding to a molecular weight of 1.95 x 106 was present in all transformants tested. Curing experiments demonstrated that the plasmid, referred to as pBY22 when present in transformants, was responsible for penicillin G and tetracycline resistance. Plasmid pBY22 was mobilized and transferred to other E. coli strains by plasmid Rldrd-19. Stability of pBY22 was examined in different E. coli strains and was shown to be stably maintained in both restriction-negative and restriction-positive strains. Unexpectedly, pBY2 and pBY22 were resistant to digestion by 12 different restriction endonucleases.

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“…The recovery of Capnocytophaga sp. known as dysgonic fermenter-1, or DF-1, was strongly associated with ulcerations of the oral mucosa (218). Entrance of the microorganisms into the circulation is believed to proceed from this site.…”

Section: Capnocytophaga Sppmentioning

confidence: 99%

Recovery of uncommon bacteria from blood: association with neoplastic disease

Beebe

Koneman

1995

Clin Microbiol Rev

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Table 6 is a summary of the organisms discussed with a listing of the environmental source, the endogenous source, the predisposing factors including neoplasms, and the postulated mechanisms by which the organism can gain access to the circulation. The evidence considered indicates that the entrance of one of these microorganisms into the bloodstream of a human being depends on the presence of multiplicity of predisposing factors. In the majority of cases of bacteremia due to one of these unusual organisms, two or more predisposing factors are present. Certain predisposing factors, such as cancer chemotherapy or intravenous catheterization, often provide a barrier break, while others, such as liver disease, may render the host immune system less capable of clearing organisms from the circulation. For organisms such as Campy-lobacter, Listeria, and Salmonella spp., attributes that allow the invasion of a healthy host are present and seem to be enhanced by the simultaneous presence of a predisposing condition, such as liver disease, in the host. Although somewhat fragmentary, a number of individual case reports describe bacteremia due to one of these organisms occurring weeks to years after surgery and after other therapeutic measures had effected a supposed cure of a cancer. It may be speculated that cancer patients, even after a cure, are still susceptible to bloodstream invasion by one of the aforementioned organisms by virtue of the presence of one or more predisposing metabolic, physiologic, or immunologic factors, even though these factors may be cryptic. The predominance of hematologic malignancies among cases of bacteremia due to these unusual organisms is also apparent. Although, as pointed out by Keusch (169), the reduction in the performance of immune function in hematologic malignancies compared with solid tumors is likely to be responsible, other associations of certain organisms with specific neoplasms warrant further examination. The frequency of bloodstream infections of Salmonella typhimurium and Capno-cytophaga canimorsus in Hodgkin's disease patients seems likely due to a particular mechanism which infection by these species is favored. The specific nature of these mechanisms remains to be determined. The recovery of any unusual bacterium from blood should warrant a careful consideration of the possibility of underlying disease, especially cancer. Microbiologists should advise clinicians of the unusual nature of the identified organism and provide the counsel that certain neoplastic processes, often accompanied by neutropenia, render the human host susceptible to invasion by almost any bacterium. The recovery of such organisms as C. septicum or S. bovis should prompt the clinician to aggressively seek to identify an occult neoplasm if one has not yet been diagnosed.

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Detection, identification, and comparison of Capnocytophaga, Bacteroides ochraceus, and DF-1

Cited by 59 publications

References 11 publications

Capnocytophaga speciesand preterm birth Case Series and Review of the Literature

Capnocytophaga speciesand preterm birth Case Series and Review of the Literature

Stability in Escherichia coli of an antibiotic resistance plasmid from Bacteroides fragilis

Recovery of uncommon bacteria from blood: association with neoplastic disease

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