Detection, characterization and identification of crucifer phytoalexins using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry

Phytoalexins and phytoanticipins from the wild crucifers Thellungiella halophila and Arabidopsis thaliana: Rapalexin A, wasalexins and camalexin

“…Â 200 mm), H 2 O-CH 3 CN, 75:25 to 100% CH 3 CN, in 35 min, linear gradient, and 1.0 ml/min flow rate; method B, Eclipse XSB C-18 column (5 lm particle size silica, 4.6 mm i.d., 150 mm), 0.2% formic acid in H 2 O and 0.2% formic acid in CH 3 CN (75% H 2 O to 75% CH 3 CN in 35 min, to 100% CH 3 CN in 5 min) and a flow rate of 1.0 ml/min. Other conditions are as previously reported (Pedras et al, 2006).…”

“…In the case of T. halophila ecotype Shandong, HPLC-DAD-MS analysis of the non-polar fraction led to the identification of several elicited metabolites by comparison of their UV spectra and retention times with those of our current phytoalexin library (Pedras et al, 2006). The elicited metabolites included the phytoalexins wasalexins A (1) and B (2), methoxybrassenin B (3), and rapalexin A (4) ( Table 1) and the phytoanticipins indolyl-3-acetonitrile (5), 4-methoxyindolyl-3-acetonitrile (6, i.e.…”

“…Column chromatography (silica gel) of the plant extracts first using a gradient of CH 2 Cl 2 -MeOH followed by preparative TLC led to identification of metabolites shown in Tables 1 and 2. Identification of each Table 3 Metabolites from Thellungiella halophila ecotypes Shandong and Yukon and Arabidopsis thaliana ecotype Columbia metabolite was carried out using NMR and HRMS spectroscopy, and HPLC-DAD-MS data, as well as direct comparison with authentic samples available in our metabolite library (Pedras et al, 2006).…”

Enantioseparation of Novel Amino Analogs of Indole Phytoalexins on Macrocyclic Glycopeptide-Based Chiral Stationary Phase

“…Recently, 23 known phytoalexins were examined to characterize their structure and construct a phytoalexins library by reversed-phase HPLC with DAD detector and electrospray ionization mass spectrometry (ESI/MS) using both negative and positive modes [7].…”

An approach on phytoalexins: function, characterization and biosynthesis in plants of the family Poaceae

“…The sakuranetin was detected by UV at a wavelength of 285nm. PEDRAS et al (2006) reported the identification of 23 cruciferous phytoalexins by HPLC analysis with diode array detection and mass spectrometry with electro spray ionization using positive and negative ion modes. According to the authors, the positive ion modes were more sensitive than the negative mode.…”