Detection by PCR of adhesins genes and slime production in clinical <i>Staphylococcus aureus</i>

Zmantar, Tarek; Chaieb, Kamel; Makni, Héla; Miladi, Hanene; Abdallah, Fethi Ben; Mahdouani, Kacem; Bakhrouf, Amina

doi:10.1002/jobm.200700289

Cited by 41 publications

(26 citation statements)

References 45 publications

(59 reference statements)

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“…They detected fnbAin all strains while the rate of fnbBwas 99%, 43%, and 0% in the 3 clones, respectively. In another study focusing on strains isolated from soft tissue infections, the rate of fnbAwas reported as 76.1% (Zmantar et al, 2008). These literature survey supports that as per the aim of the present study, the genes encoding the virulence factors play a role in the pathogeneses of community acquired musculoskeletal and skin/soft tissue infections.…”

Section: Antibacterial Activity Of Herbal-silver Nanocomposite Hs Nc supporting

confidence: 87%

Development of Herbal-Silver Nano Composite (HSNC): Antibacterial Evaluation and Investigation of fnb A and fnb B Genes Coding Fibronectin-binding Proteins in Staphylococcus aureus

Meenakshi¹,

Gandhi²

2016

Int.J.Curr.Microbiol.App.Sci

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Section: Antibacterial Activity Of Herbal-silver Nanocomposite Hs Nc supporting

confidence: 87%

Development of Herbal-Silver Nano Composite (HSNC): Antibacterial Evaluation and Investigation of fnb A and fnb B Genes Coding Fibronectin-binding Proteins in Staphylococcus aureus

Meenakshi¹,

Gandhi²

2016

Int.J.Curr.Microbiol.App.Sci

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“…Qualitative detection of slime formation was studied by culturing the isolated strains on Congo red agar plate (CRA) as previously described (Chaieb et al 2007;Zmantar et al 2008). After incubation, black colonies were considered as slime positive, whereas slime-negative strains formed pinkish red, smooth colonies with a darkening at the center.…”

Section: Biofilm Formationmentioning

confidence: 99%

Atomic force microscopy and hydrodynamic characterization of the adhesion of staphylococcus aureus to hydrophilic and hydrophobic substrata at different pH values

Zmantar

Bettaieb

Chaieb

et al. 2010

World J Microbiol Biotechnol

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Understanding the mechanism of the bacterial cell adhesion to solid surfaces is of great medical and industrial importance. Bacterial adhesion to inert surfaces, such as a catheter, and other indwelling devices can form biofilm, consequently cause severe morbidity and often fatal infections. Initial bacterial adhesion to the material surfaces is a complicated process that is affected by various physicochemical properties of both bacterial cells and substratum surfaces. The surface properties of the cells were characterized by the sessile drop technique. Moreover, the interfacial free energy of Staphylococcus aureus adhesion to the supporting materials was determined. The results showed that S. aureus examined at different pH levels could be considered hydrophilic. We noted hat the electron-donor character of S. aureus was important at intermediate pH (pH 5, pH 7, and pH 9) and it decreased at both limits acidic and basic conditions. In addition, the adhesion of Staphylococcus aureus ATCC 25923 to the hydrophilic glass and hydrophobic indium tin oxide (ITO)-coated glass surfaces at different pH values (2, 3, 5, 7, 9 and 11) was investigated using atomic force microscopy (AFM) and image analysis was assessed with the Mathlab Ò program. The data analysis showed that cells (number of adhering cells to glass and ITO-coated glass surface) adhered strongly at acidic pH and weakly at alkaline pH. Also, S. aureus has the ability to attach to both hydrophobic and hydrophilic surfaces, but the adhesion was higher on hydrophobic surface.

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“…Structurally, both PIA and PS/A are similar with a common backbone of β-1-6-linked polyglucosamine, but differ in the primary substitutions in their amino groups. The intercellular adhesion (ica) locus consisting of the genes icaADB and C encodes the proteins mediating the synthesis of PIA and PS/A in staphylococcal species (Cramton et al 1999;Zmantar et al 2008). Among the ica genes, icaA and icaD have been reported to a play a significant role in biofilm formation in S. aureus.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of intercellular adhesion gene in Staphylococcus aureus isolated from bovine mastitic milk

Chavhan

Nagdive

Purohit

et al. 2011

Trop Anim Health Prod

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Biofilm formation in Staphylococcus aureus is considered an important virulence factor in bovine mastitis. Intercellular adhesion gene A (icaA) is a significant genetic determinant that contributes in biofilm formation. The aim of the present study was to determine the presence of the icaA gene in S. aureus isolated from bovine mastitis from seven states of India. A total of 88 out of 150 Staphylococcus aureus strains were found to be positive for biofilm marker icaA gene by PCR. The icaA gene was confirmed by dot blot hybridization in 41 of 150 S. aureus strains tested. Results obtained with dot blot hybridization were comparable to those obtained with PCR. Partial sequences of the icaA gene of the two S. aureus isolates showed deletion of some bases in different positions that might reduce/stop transcription leading to no biofilm formation. PCR was found to be a rapid test but dot blot hybridization was more accurate than PCR for detection of icaA genes. This study showed that detection of biofilm marker the icaA gene in S. aureus would allow the detection of virulence factors present in mastitis and early application of corrective measures.

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Detection by PCR of adhesins genes and slime production in clinical Staphylococcus aureus

Cited by 41 publications

References 45 publications

Development of Herbal-Silver Nano Composite (HSNC): Antibacterial Evaluation and Investigation of fnb A and fnb B Genes Coding Fibronectin-binding Proteins in Staphylococcus aureus

Development of Herbal-Silver Nano Composite (HSNC): Antibacterial Evaluation and Investigation of fnb A and fnb B Genes Coding Fibronectin-binding Proteins in Staphylococcus aureus

Atomic force microscopy and hydrodynamic characterization of the adhesion of staphylococcus aureus to hydrophilic and hydrophobic substrata at different pH values

Molecular characterization of intercellular adhesion gene in Staphylococcus aureus isolated from bovine mastitic milk

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