Detection by ELISA of the humoral immune response in rabbits naturally infected with Trichophyton mentagrophytes

Zrimšek, Petra; Kos, Janko; Pinter, Ljiljana; Drobnič‐Košorok, M.

doi:10.1016/s0378-1135(99)00125-x

Cited by 13 publications

(7 citation statements)

References 17 publications

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“…When re‐sampled 2 weeks later, the three dogs still had culture‐positive lesions and their blots exhibited dominant bands. Given these results and previous reports showing nearly 100% sensitivity for serological tests of animals with chronic infection, 12,13,26 and a significant correlation between infection duration and IgG concentration, 12 the three dogs were likely sampled before seroconversion. This possibility can be confirmed by the fact that the nine dominant bands were found in all the blots from subjects with longer infection (more than 30 days as determined from the anamnesis, Fig.…”

Section: Discussionsupporting

confidence: 64%

Use of western blot to study Microsporum canis antigenic proteins in canine dermatophytosis

Peano

Min

Beccati³

et al. 2011

Mycoses

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Western blotting was used to describe the Microsporum canis proteins with antigenic activity in dogs with dermatophytosis. Electrophoretic separation of whole fungal strain extract cultured from a cat was performed under denaturing conditions. The proteins were blotted onto nitrocellulose and probed with sera collected from 22 dogs with dermatophytosis (18 M. canis, 3 M. gypseum, 1 Trichophyton mentagrophytes; group A), 20 dogs with skin diseases other than dermatophytosis, and 22 dogs with no clinical cutaneous signs (group B, n = 42). Nine principal IgG-binding proteins with apparent molecular weights of 180, 144, 130, 120, 102, 96, 80, 68, and 48 kD were visualised on group A blots. For these proteins, serological cross-reactivity with different strains of M. canis may be indirectly confirmed, whereas additional proteins were found to react with sera from individual dogs. The proteins visualised in this study may represent diagnostic markers of dermatophyte infection. The proteins should be further evaluated for their role in the cellular immune response of dogs with dermatophytosis.

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Section: Discussionsupporting

confidence: 64%

Use of western blot to study Microsporum canis antigenic proteins in canine dermatophytosis

Peano

Min

Beccati³

et al. 2011

Mycoses

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“…An ELISA test has presented the same sensitivity value in chronic dermatophytic cats. 9 In the rabbits described above, 11 ELISA sensitivity was 96% 11 weeks after infection. A window probably exists between onset of clinical signs and seroconversion during dermatophytosis in some dogs.…”

Section: Discussionmentioning

confidence: 99%

“…7 The production of specific antibodies has been demonstrated in humans, cats, guinea pigs, rabbits and dogs. [8][9][10][11][12] At the moment no commercial test is available for the serological diagnosis of dermatophytosis. The aim of this study was to develop an enzyme-linked immunosorbant assay (ELISA) for the serological diagnosis of dermatophytosis in dogs.…”

Section: Introductionmentioning

confidence: 99%

Development of an enzyme‐linked immunosorbant assay (ELISA) for the serodiagnosis of canine dermatophytosis caused byMicrosporum canis

Peano

Rambozzi

Gallo

2005

Veterinary Dermatology

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In dogs, dermatophytosis should be considered in any case of alopecic, papular or pustular lesion. The aim of this study was to develop an enzyme-linked immunosorbant assay (ELISA) as an aid in the diagnosis of canine dermatophytosis. The antigen used was a whole fungal extract obtained from an isolate of Microsporum canis cultured on a liquid medium from the parasitized hair of a cat with patches of alopecia. To assess the ELISA performances, sera from 18 dogs with dermatophytosis caused by M. canis (group A, n = 18), 20 dogs with skin diseases other than dermatophytosis and 22 healthy dogs (group B, n = 42) were tested. Four further animals were tested: three with dermatophytosis caused by M. gypseum and one by T. mentagrophytes. A significant difference (P < 0.01, Wilcoxon's test, w = 364) was found between IgG-specific levels of sera of recently M. canis-infected dogs (infection < 15 days) and controls (although three dogs had negative titres at this stage). A highly significant difference (P < 0.001, w = 462) was noted between controls and dogs with infection of longer duration (> 30 days). All dogs had positive titres at this stage. A highly significant correlation (P < 0.001, Spearman's test, rho = 0.86) between duration of infection and IgG concentration was noted. The test has good sensitivity (83.3%) and high specificity (95.2%) but some dogs retained positive titres after elimination of infection. The sensitivity is higher than that of direct microscopic hair examination and similar to that of fungal culture with DTM (dermatophyte test medium).

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“…Recently, an indirect ELISA was developed and its diagnostic potential evaluated for rabbits infected by T. mentagrophytes; this test may prove useful in earlier detection of the disease. 23 Treatment, which should include all in-contact mammals, includes topical enilconazole (0.2% solution twice weekly for 3 weeks; not available in the United States) or lime sulfur. Systemic griseofulvin (15 to 25 mg/kg PO for 4 weeks) is also very effective.…”

Section: Alopecia Dermatophytesmentioning

confidence: 99%

Dermatologic problems of rabbits

White

Bourdeau

Meredith

2002

Seminars in Avian and Exotic Pet Medicine

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Detection by ELISA of the humoral immune response in rabbits naturally infected with Trichophyton mentagrophytes

Cited by 13 publications

References 17 publications

Use of western blot to study Microsporum canis antigenic proteins in canine dermatophytosis

Use of western blot to study Microsporum canis antigenic proteins in canine dermatophytosis

Development of an enzyme‐linked immunosorbant assay (ELISA) for the serodiagnosis of canine dermatophytosis caused byMicrosporum canis

Dermatologic problems of rabbits

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