Detection and verification of quantitative trait loci for resistance to Fusarium ear rot in maize

Chen, Jiafa; Ding, Junqiang; Li, Huimin; Li, Zhimin; Sun, Xiaodong; Li, Jingjing; Wang, Ruixia; Dai, Xiaodong; Dong, Hua-Fang; Song, Weibin; Chen, Wei; Zhang, Xia; Wu, Jianyu

doi:10.1007/s11032-012-9748-1

Cited by 59 publications

(50 citation statements)

References 26 publications

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“…The genetic mechanisms for F. verticillioides resistance had some reported (Yuan et al, 2013; Ridenour et al, 2016), however, the QTLs associated with F. verticillioides resistance usually have small effects and are not stable in different populations (Pérez-Brito et al, 2001; Robertson-Hoyt et al, 2006; Ding et al, 2008; Xiang et al, 2010; Li et al, 2011; Mesterházy et al, 2012). In our previous research, a stable QTL on chromosome 4 was detected by two populations in different environments and verified by near-isogenic lines (Chen et al, 2012). The common resistant parent (BT-1) of two populations showed strengthened resistance, close to immunity, to the ear rot and stalk rot caused by F. verticillioides , and thus is an ideal material for study of maize resistance against F. verticillioides .…”

Section: Introductionmentioning

confidence: 74%

“…Extensive studies of ear rot resistance in maize have identified many related QTLs as well as highly resistant materials (Pérez-Brito et al, 2001; Robertson-Hoyt et al, 2006; Zhang et al, 2006; Ding et al, 2008; Xiang et al, 2010; Li et al, 2011; Chen et al, 2012). To data, however, any of genes or QTLs related to ear rot resistance has not been cloned or verified in maize, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…The susceptible parental line N6 was an improved inbred line from huangzaosi which has high combining ability, good plant type, short growth period and high stress resistance with weak disease resistance. BT-1 and N6 have been identified as one of the best materials which showed the highest resistant and susceptible to Furiasum ear rot F. verticillioides , respectively (Chen et al, 2012; Yuan et al, 2013). The maize kernels were inoculated with F. verticillioides , and with water as the control, on day 15 after pollination.…”

Section: Methodsmentioning

confidence: 99%

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The Mechanisms of Maize Resistance to Fusarium verticillioides by Comprehensive Analysis of RNA-seq Data

et al. 2016

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Fusarium verticillioides is the most commonly reported fungal species responsible for ear rot of maize which substantially reduces grain yield. It also results in a substantial accumulation of mycotoxins that give rise to toxic response when ingested by animals and humans. For inefficient control by chemical and agronomic measures, it thus becomes more desirable to select more resistant varieties. However, the molecular mechanisms underlying the infection process remain poorly understood, which hampers the application of quantitative resistance in breeding programs. Here, we reveal the disease-resistance mechanism of the maize inbred line of BT-1 which displays high resistance to ear rot using RNA high throughput sequencing. By analyzing RNA-seq data from the BT-1 kernels before and after F. verticillioides inoculation, we found that transcript levels of genes associated with key pathways are dramatically changed compared with the control treatment. Differential gene expression in ear rot resistant and susceptible maize was confirmed by RNA microarray and qRT-PCR analyses. Further investigation suggests that the small heat shock protein family, some secondary metabolites, and the signaling pathways of abscisic acid, jasmonic acid, or salicylic acids (SA) may be involved in the pathogen-associated molecular pattern-triggered immunity against F. verticillioides. These data will not only provide new insights into the molecular resistant mechanisms against fungi invading, but may also result in the identification of key molecular factors associated with ear rot resistance in maize.

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Section: Introductionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The Mechanisms of Maize Resistance to Fusarium verticillioides by Comprehensive Analysis of RNA-seq Data

et al. 2016

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“…Surrounding regions, bin 2.08, also seem to be important in temperate populations growing in the Corn Belt of US (Robertson-Hoyt et al 2006b) and China, bins 2.01 and 2.02 (Zhang et al 2007). Quantitative trait loci for Fusarium ear rot resistance in chromosomes 3 and 5 were reported in bins 3.04, 3.08, 5.04 and 5.07 from sources of resistance growing in China (Zhang et al 2007;Ding et al 2008;Chen et al 2012), and in bins 3.06, 5.05 and 5.06 from the US (Robertson-Hoyt et al 2006b). For Gibberella ear rot resistance, QTLs were reported in bins 2.04, 2.06 and 2.08, 3.02, 3.04, 3.05, 3.06, 5.04, 5.05 and 5.06 in sources of resistance from Canada, Germany and Uganda (Liakat Ali et al 2005;Martin et al 2011Martin et al , 2012Tembo et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Quantitative trait loci for Fusarium and Gibberella ear rot resistance in Argentinian maize germplasm

Giomi

Kreff²,

Iglesias

et al. 2016

Euphytica

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Fusarium verticillioides and F. graminearum cause ear rots in maize (Zea mays L.) that reduce yield and contaminate the grain with mycotoxins produced by the fungi. To map QTLs for resistance to these ear rots, a F 5 mapping population, consisting of 298 recombinant inbreds obtained by randomly selfing of the cross between LP4637 (moderately resistant) and L4674 (susceptible), was genotyped with 250 single nucleotide polymorphism markers and phenotyped 2 years for disease severity after silk inoculation with conidial suspensions of F. verticillioides and F. graminearum. Four QTLs were mapped in chromosomes 2, 3 and 5, bins 2.03, 3.05, 3.07 and 5.07, explaining ranges of 11.2-11.8, 3.4-5.1, 6.2-7.6 and 3.8-5.0 of phenotypic variances (%), respectively, depending on year and fungus. Additive effects of each QTL ranged from 5.0 to 11.9 % of ear area covered by mold and no epistatic interactions were observed. The four QTLs were effective for both Fusarium species and environments indicating that LP4637 is a source of broad resistance to Fusarium stable across environments. These results are consistent with previous research reporting QTLs for ear rot resistance in the same chromosome regions from sources of resistance growing in North America, Africa, Europe and China.

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“…However, maize ear rot caused by various fungi can severely reduce the yield and quality of maize (Chen et al 2012;Yuan et al 2013). Among these fungi, Fusarium species are considered the major causal agents of maize ear rot (Goertz et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Fusariumspecies identification and fumonisin production in maize kernels from Shandong Province, China, from 2012 to 2014

Guo

Liu

Jiang

et al. 2016

Food Additives & Contaminants: Part B

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A total of 225 maize kernel samples were collected from Shandong Province in China from 2012 to 2014 and analysed for contamination with Fusarium spp. and fumonisins (FBs) using molecular methods and high-performance liquid chromatography with fluorescence detection. The results showed that the average incidences of Fusarium spp. in 2012, 2013 and 2014 were 23.3%, 37.1% and 36.5%, respectively, Fusarium verticillioides being the predominant species. In 2012, the average contamination level of FBs was 3071 ng g(-1), which was higher than that in 2014 (2913 ng g(-1)) and 2013 (2072 ng g(-1)). Of all samples, 13% and 19% had FB contamination levels higher than 2000 and 4000 ng g(-1), which are the maximum limits as set by the Food and Drug Administration of the United States and the European Commission, respectively. Therefore, efforts should be taken to minimise the potential risk of FBs to the health of humans and animals.

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Detection and verification of quantitative trait loci for resistance to Fusarium ear rot in maize

Cited by 59 publications

References 26 publications

The Mechanisms of Maize Resistance to Fusarium verticillioides by Comprehensive Analysis of RNA-seq Data

The Mechanisms of Maize Resistance to Fusarium verticillioides by Comprehensive Analysis of RNA-seq Data

Quantitative trait loci for Fusarium and Gibberella ear rot resistance in Argentinian maize germplasm

Fusariumspecies identification and fumonisin production in maize kernels from Shandong Province, China, from 2012 to 2014

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