Detection and Typing of
            <i>Campylobacter jejuni</i>
            and
            <i>Campylobacter coli</i>
            and Analysis of Indicator Organisms in Three Waterborne Outbreaks in Finland

Hänninen, Marja‐Liisa; Haajanen, H.; Pummi, T.; Wermundsen, K.; Katila, Marja–Leena; Sarkkinen, Hannu; Miettinen, Ilkka T.; Rautelin, Hilpi

doi:10.1128/aem.69.3.1391-1396.2003

Cited by 151 publications

(137 citation statements)

References 21 publications

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“…Campylobacters, Escherichia coli, or other coliforms have rarely been detected in potential sources. Using a combination of Penner serotyping and pulsedfield gel electrophoresis (digestion with SmaI and KpnI), Hanninen et al [57] studied three waterborne outbreaks in Finland caused by C. jejuni and used sample volumes of 4 000 to 20 000 mL for analysis of Campylobacters and sample volumes of 1 to 5 000 mL for analysis of coliforms and E. coli, depending on the sampling site, confirming the likely reservoir of an outbreak. Poor water quality, sanitation and hygiene account for some 1.7 million deaths a year world-wide (3.1% of all deaths and 3.7% of all DALY's), mainly through infectious diarrhoea.…”

Section: Watermentioning

confidence: 99%

Campylobacter

et al. 2005

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-Species within the genus, Campylobacter, have emerged over the last three decades as significant clinical pathogens, particularly of human public health concern, where the majority of acute bacterial enteritis in the Western world is due to these organisms. Of particular concern are the species, C. jejuni and C. coli, which are responsible for most of these gastrointestinal-related infections. Although these organisms have already emerged as causative agents of zoonoses, several aspects of their epidemiology and pathophysiology are only beginning to emerge. Trends in increasing antibiotic resistance are beginning to emerge with oral antibiotics, which may be the drug of choice for when it is necessary to intervene chemotherapeutically. This review wishes to examine (i) emerging clinical aspects of the disease, such as Guillain Barré syndrome (GBS), (ii) the association between these organisms and poultry as a natural host, (iii) environmental aspects of Campylobacter epidemiology, (iv) the emergence of atypical campylobacters (v) emerging trends in antibiotic resistance, (vi) adoption of modern methods for the detection of campylobacters.

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Section: Watermentioning

confidence: 99%

Campylobacter

et al. 2005

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“…Vehicles including milk, fatty foods and water may facilitate the transfer of the bacterium through the gastric acid barrier and so allow some infections to occur at a relatively low infectious dose [2]. Continuous common-source outbreaks are usually associated with contaminated water supplies [3,4]. Only one foodborne continuous common-source outbreak of campylobacteriosis, due to the distribution of contaminated chicken from a single poultry producer, has been reported in the literature [5].…”

Section: Introductionmentioning

confidence: 99%

A continuous common-source outbreak of campylobacteriosis associated with changes to the preparation of chicken liver pâté

et al. 2008

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“…The bacterium itself is a largely zoonotic and does not usually reproduce in foods [4]. The host, in which it colonizes, ranges from wild birds to domestic animals [5,6]. In most cases, the bacterium multiplies in chicken and the animal become a reservoir of the pathogen [7].…”

Section: Introductionmentioning

confidence: 99%

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

2017

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Abstract:Campylobacteriosis is an internationally important foodborne disease caused by Campylobacter jejuni. The bacterium is prevalent in chicken meat and it is estimated that as much as 90% of chicken meat on the market may be contaminated with the bacterium. The current gold standard for the detection of C. jejuni is the culturing method, which takes at least 48 h to confirm the presence of the bacterium. Hence, the aim of this work was to investigate the development of a Surface Plasmon Resonance (SPR) sensor platform for C. jejuni detection. Bacterial strains were cultivated in-house and used in the development of the sensor. SPR sensor chips were first functionalized with polyclonal antibodies raised against C. jejuni using covalent attachment. The gold chips were then applied for the direct detection of C. jejuni. The assay conditions were then optimized and the sensor used for C. jejuni detection, achieving a detection limit of 8 × 10 6 CFU·mL −1 . The sensitivity of the assay was further enhanced to 4 × 10 4 CFU·mL −1 through the deployment of a sandwich assay format using the same polyclonal antibody. The LOD obtained in the sandwich assay was higher than that achieved using commercial enzyme-linked immunosorbent assay (ELISA) (10 6 -10 7 CFU·mL −1 ). This indicate that the SPR-based sandwich sensor method has an excellent potential to replace ELISA tests for C. jejuni detection. Specificity studies performed with Gram-positive and Gram-negative bacteria, demonstrated the high specific of the sensor for C. jejuni.

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Detection and Typing of Campylobacter jejuni and Campylobacter coli and Analysis of Indicator Organisms in Three Waterborne Outbreaks in Finland

Cited by 151 publications

References 21 publications

Campylobacter

Campylobacter

A continuous common-source outbreak of campylobacteriosis associated with changes to the preparation of chicken liver pâté

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

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