Detection and transfer of antimicrobial resistance gene integron in Salmonella Enteritidis derived from avian material

Abstract

The expansion of global poultry production has increased the need to reduce or control the agents responsible for economic losses, including Salmonella spp. These bacteria are also of public health concern due to their potential to cause food poisoning, and, more recently, due to the antimicrobial resistance presented by these bacteria. Molecular biology is an important tool currently used in the diagnosis and research studies of main poultry diseases. The present studied analyzed 100 samples of Salmonella Enteritidis (SE) isolated from avian material aiming at detecting the class 1 integron gene, Integroninvolved in antimicrobial resistance, by means of polymerase chain reaction (PCR), and comparing it with plate inhibition test. Subsequently, SE samples were evaluated for their capacity to horizontally transfer this gene. There was no direct relationship between the presence of the class 1 integron gene and SE resistance to the 14 antimicrobials tested, as 80% of the studied samples were resistant to up to three antimicrobials, and did not present the aforementioned gene. However, horizontal transfer of this gene was accomplished in vitro (from Escherichia coli to Salmonella Enteritidis), demonstrating that capacity class 1 integron gene can be disseminated among enterobacteria

Characteristics ofSalmonellaspp. Isolated from Wild Birds Confiscated in Illegal Trade Markets, Rio de Janeiro, Brazil

“…Panama strains mediates resistance to gentamicin. Despite the detection of phenotypic resistance to multiple antibiotics, most different serotypes isolated from clinical cases are resistant to various antimicrobials and carry the class 1 Integron gene, involved in antimicrobial multiresistance [ 36 ]. This gene was not observed in any samples analyzed by PCR.…”

Characterization of class I integrons among Salmonella enterica serovar Enteritidis isolated from humans and poultry

“…Similar results were observed by Okamoto et al . (), who demonstrated that 80% of S. Enteritidis isolates from avian sources in Brazil were MDR. Despite predominance of this serovar, the level of antimicrobial resistance in S. Enteritidis isolated from food animal or human sources in European countries is relatively low in comparison with levels in developing countries including Iran (Naghoni et al ., ).…”

Genetic Characterization of blaCTX–M–55 -Bearing Plasmids Harbored by Food-Borne Cephalosporin-Resistant Vibrio parahaemolyticus Strains in China

“…are known to be susceptible to most clinically used antibiotics (Shaw et al, 2014; Letchumanan et al, 2015a). It is noted that most of the antibiotic resistance determinants are located in the plasmid, which is the most important mediator that facilitate the transfer of antibiotic resistant genes (Okamoto et al, 2009; Letchumanan et al, 2015b). IncC group plasmids have been identified in various species, indicating that such plasmids have been disseminated to, and accommodated by a broad range of bacterial hosts since they were first described among multidrug resistant Aeromonas hydrophila causing disease in cultured fish in the 1970s (Fricke et al, 2009; Sekizuka et al, 2011; Harmer and Hall, 2015; Li et al, 2015a).…”