Detection and tissue distribution of potato spindle tuberviroid in infected tomato plants by tissue print hybridization

Abstract

Potato spindle tuber viroid (PSTVd) was detected in two cultivars of tomato (Lycopersicon esculentum Mill.) by tissue print hybridization of cross-sections of stem and rhachis, using a 35S-labeled PSTVd RNA probe. PSTVd was detectable in the viroid-sensitive and symptom-developing cv "Rutgers" 2 weeks p.i., and in the viroid-tolerant and practically symptomless cv "Goldkugel" 3 weeks p.i. In both tomato cultivars, PSTVd accumulated in the upper parts of the plants newly grown after inoculation. It was predominantly found in association with the ring formed by the vascular tissue. The final accumulation of PSTVd as well as its spatial distribution were similar in the sensitive and in the tolerant tomato cultivar, as estimated from the tissue print autoradiographs. Thus, tissue print hybridization provides a rapid and sensitive means for viroid diagnosis and for the assessment of tissue-specific localization of the viroid RNA.

The R2R3-MYB Transcription Factors MYB14 and MYB15 Regulate Stilbene Biosynthesis in Vitis vinifera

“…The introduction of Vv-STS41 (formerly pVst1) into other plant species, such as tobacco, under the control of its own promoter, induced the production of stilbenes, suggesting there are common TFs present in these other plant species, which do not contain STS genes (Hain et al, 1990;Stark-Lorenzen et al, 1997;Thomzik et al, 1997;Zhu et al, 2005). A number of MYB factors orthologous to Vv-MYB14 and Vv-MYB15, which have been detected in other known stilbene-producing plants, such as sorghum and Picea glauca (see Supplemental Figure 2B, Supplemental Methods 1, and Supplemental References 1 online; i.e., SORBIDRAFT_06g022660; PgMYB12), would also be interesting candidates for studying the regulation of stilbene biosynthesis in these plants.…”

Viroid RNA systemic spread may depend on the interaction of a 71-nucleotide bulged hairpin with the host protein VirP1

“…The maize trancription factor KNOTTED1 transports the knotted1 mRNA through plasmodesmata in the developing mesophyll (Lucas et al 1995). VirP1 may additionally (or alternatively) contribute to the intercellular transport of PSTVd RNA, which moves through plasmodesmata (Ding et al 1997) and uses the phloem (Palukaitis 1987), where it can be detected (Stark-Lorenzen et al 1997) and replicated (Zhu et al 2001). Perhaps VirP1 allows access of the viroid to pathways that propagate systemically endogenous RNA signals.…”

Analysis of immunogenicity of minor envelope protein GP3 of porcine reproductive and respiratory syndrome virus in mice

“…Viroid detection mainly depends either on time-consuming biological assays or on molecular techniques aimed to detect the presence of their RNA genomes [3]. Commonly applied techniques are the direct visualization of viroid RNAs after gel electrophoresis [10,11], molecular hybridization [12][13][14][15][16][17][18][19] and PCR amplification [20][21][22][23][24][25][26][27][28]. The choice and applicability of these methods for different viroids depends on the diagnostic purposes, the availability of trained personnel and viroid titer in infected tissues.…”